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. 1987 Aug;389:147–161. doi: 10.1113/jphysiol.1987.sp016651

The role of myogenic relaxation, adenosine and prostaglandins in human forearm reactive hyperaemia.

I Carlsson 1, A Sollevi 1, A Wennmalm 1
PMCID: PMC1192075  PMID: 3681724

Abstract

1. Forearm blood flow was measured bilaterally in healthy young male and female volunteers, in the basal state and after upper-arm occlusion of arterial or venous blood flow for 1-20 min. The investigations were repeated after pre-treatment with drugs affecting vascular prostaglandins and/or adenosine. 2. Simultaneous arterial occlusion in one arm and venous occlusion in the contralateral arm for up to 20 min elicited a considerable reactive hyperaemia in the arm subjected to arterial occlusion, but completely failed to elevate the post-occlusive flow in the arm subjected to venous occlusion above the pre-occlusive level. 3. When the arterial occlusion was increased from 1 to 20 min there was a progressive increase in the subsequent reactive hyperaemia, up to 30 ml 100 ml tissue-1. The time dependence following 1-3 min of arterial occlusion was based on a facilitation of the peak post-occlusive flow, while prolongation of the arterial occlusion from 3 to 20 min augmented the reactive hyperaemia mainly by increasing its duration. 4. Inhibition of prostaglandin synthesis with ibuprofen reduced the total reactive hyperaemia following 3-5 min of arterial occlusion by up to 70%. This attenuation was due both to a reduction of peak post-occlusive flow and to a shortening of the duration of the post-occlusive hyperaemia. 5. The adenosine receptor antagonist theophylline reduced the reactive hyperaemia following 5 min of arterial occlusion by about 35%. Combined treatment with ibuprofen and theophylline did not reduce the reactive hyperaemia more than either drug alone. 6. Infusion of dipyridamole, a drug which inhibits the elimination of adenosine, reinforced the reactive hyperaemia by about 45%. This effect of dipyridamole was completely inhibited by administration of theophylline, and also by ibuprofen. 7. Plasma levels of adenosine, hypoxanthine and uric acid were maintained during the reactive hyperaemia, indicating increased production of purines during or immediately after the ischaemia. 8. It is concluded that the adequate stimulus for vascular relaxation in response to interruption of blood flow is omission of vessel wall distension. Local metabolic factors like endogenously formed prostaglandins and adenosine may act synergistically to this myogenic response but seem to be inactive alone. The lack of additive effects of ibuprofen and theophylline suggests a link between vascular relaxation induced by prostaglandins and by adenosine.

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Selected References

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  1. Bayliss W. M. On the local reactions of the arterial wall to changes of internal pressure. J Physiol. 1902 May 28;28(3):220–231. doi: 10.1113/jphysiol.1902.sp000911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carlsson I., Wennmalm A. Effect of different prostaglandin synthesis inhibitors on post-occlusive blood flow in human forearm. Prostaglandins. 1983 Aug;26(2):241–252. doi: 10.1016/0090-6980(83)90092-8. [DOI] [PubMed] [Google Scholar]
  3. Ciabattoni G., Wennmalm A. Adenosine-induced coronary release of prostacyclin at normal and low pH in isolated heart of rabbit. Br J Pharmacol. 1985 Jun;85(2):557–563. doi: 10.1111/j.1476-5381.1985.tb08893.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Degenring F. H., Curnish R. R., Rubio R., Berne R. M. Effect of dipyridamole on myocardial adenosine metabolism and coronary flow in hypoxia and reactive hyperemia in the isolated perfused guinea pig heart. J Mol Cell Cardiol. 1976 Nov;8(11):877–888. doi: 10.1016/0022-2828(76)90070-5. [DOI] [PubMed] [Google Scholar]
  5. Dobson J. G., Jr, Rubio R., Berne R. M. Role of adenine nucleotides, adenosine, and inorganic phosphate in the regulation of skeletal muscle blood flow. Circ Res. 1971 Oct;29(4):375–384. doi: 10.1161/01.res.29.4.375. [DOI] [PubMed] [Google Scholar]
  6. Fredholm B. B., Sollevi A. The release of adenosine and inosine from canine subcutaneous adipose tissue by nerve stimulation and noradrenaline. J Physiol. 1981;313:351–367. doi: 10.1113/jphysiol.1981.sp013670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HILTON S. M. Experiments on the post contraction hyperaemia of skeletal muscle. J Physiol. 1953 Apr 28;120(1-2):230–245. doi: 10.1113/jphysiol.1953.sp004888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Herbaczyńska-Cedro K., Staszewska-Barczak J., Janczewska H. The release of prostaglandin-like substances during reactive and functional hyperemia in the hind leg of the dog. Pol J Pharmacol Pharm. 1974 Jan-Apr;26(1):167–170. [PubMed] [Google Scholar]
  9. Hester R. L., Guyton A. C., Barber B. J. Reactive and exercise hyperemia during high levels of adenosine infusion. Am J Physiol. 1982 Aug;243(2):H181–H186. doi: 10.1152/ajpheart.1982.243.2.H181. [DOI] [PubMed] [Google Scholar]
  10. Kilbom A., Wennmalm A. Endogenous prostaglandins as local regulators of blood flow in man: effect of indomethacin on reactive and functional hyperaemia. J Physiol. 1976 May;257(1):109–121. doi: 10.1113/jphysiol.1976.sp011358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Messina E. J., Weiner R., Kaley G. Arteriolar reactive hyperemia: modification by inhibitors of prostaglandin synthesis. Am J Physiol. 1977 Jun;232(6):H571–H575. doi: 10.1152/ajpheart.1977.232.6.H571. [DOI] [PubMed] [Google Scholar]
  12. Nowak J., Wennmalm A. A study on the role of endogenous prostaglandins in the development of exercise-induced and post-occlusive hyperemia in human limbs. Acta Physiol Scand. 1979 Jul;106(3):365–369. doi: 10.1111/j.1748-1716.1979.tb06411.x. [DOI] [PubMed] [Google Scholar]
  13. PATTERSON G. C., WHELAN R. F. Reactive hyperaemia in the human forearm. Clin Sci. 1955 May;14(2):197–211. [PubMed] [Google Scholar]
  14. Pedersen A. K. Specific determination of dipyridamole in serum by high-performance liquid chromatography. J Chromatogr. 1979 Jan 1;162(1):98–103. doi: 10.1016/s0378-4347(00)82069-1. [DOI] [PubMed] [Google Scholar]
  15. Rubio R., Berne R. M. Release of adenosine by the normal myocardium in dogs and its relationship to the regulation of coronary resistance. Circ Res. 1969 Oct;25(4):407–415. doi: 10.1161/01.res.25.4.407. [DOI] [PubMed] [Google Scholar]
  16. Sollevi A., Fredholm B. B. Role of adenosine in adipose tissue circulation. Acta Physiol Scand. 1981 Jul;112(3):293–298. doi: 10.1111/j.1748-1716.1981.tb06819.x. [DOI] [PubMed] [Google Scholar]
  17. Sollevi A., Ostergren J., Fagrell B., Hjemdahl P. Theophylline antagonizes cardiovascular responses to dipyridamole in man without affecting increases in plasma adenosine. Acta Physiol Scand. 1984 Jun;121(2):165–171. doi: 10.1111/j.1748-1716.1984.tb07443.x. [DOI] [PubMed] [Google Scholar]
  18. Sparks H. V., Jr, Belloni F. L. The peripheral circulation: local regulation. Annu Rev Physiol. 1978;40:67–92. doi: 10.1146/annurev.ph.40.030178.000435. [DOI] [PubMed] [Google Scholar]
  19. Tabaie H. M., Scott J. B., Haddy F. J. Reduction of exercise dilation by theophylline. Proc Soc Exp Biol Med. 1977 Jan;154(1):93–97. doi: 10.3181/00379727-154-39611. [DOI] [PubMed] [Google Scholar]

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