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. 1987 Oct;391:407–418. doi: 10.1113/jphysiol.1987.sp016745

Renal haemodynamic actions of pressor doses of lysine vasopressin in the rat.

C J Lote 1, A J McVicar 1, A Thewles 1
PMCID: PMC1192221  PMID: 3443952

Abstract

1. Dose-response effects of lysine vasopressin on renal haemodynamics were studied in conscious rats infused with 2.5% (w/v) dextrose solution at 5.8 ml/h. 2. Lysine vasopressin was maximally antidiuretic in the absence of a significant pressor effect at a dose of 2.5 pmol h-1 100 g body weight-1. Doses of vasopressin greater than this induced a dose-dependent increase in arterial blood pressure. 3. The clearance of p-aminohippurate (PAH) was not significantly changed by vasopressin, even at pressor doses. Rats pre-treated with indomethacin to inhibit prostaglandin synthesis showed a decrease in PAH clearance during the infusion of vasopressin at a dose of 30 pmol h-1 100 g body weight-1, and this suggests that the renal vasoconstrictor actions of vasopressin are attenuated by dilator prostaglandins. 4. Inulin clearance was unchanged by non-pressor doses of vasopressin but was decreased in a dose-dependent manner by pressor doses. A maximal effect was induced by a dose of 30 pmol h-1 100 g body weight-1 which decreased inulin clearance from 3.23 +/- 0.76 (mean +/- S.E. of mean) to 1.60 +/- 0.37 ml/min (P less than 0.02). A change in inulin clearance (from 3.42 +/- 0.46 to 2.17 +/- 0.33 ml/min, P less than 0.01) was also observed in rats pre-treated with indomethacin and infused with vasopressin at the same dose. The magnitude of the change was not significantly different from that observed in rats which were not treated with indomethacin. 5. Control rats infused with dextrose showed a slight but significant increase in sodium excretion during the course of the experiment. A similar natriuresis was observed in rats infused with non-pressor doses of vasopressin but was considerably enhanced in rats infused with pressor doses of the peptide. The antidiuresis induced by vasopressin remained maximal in rats infused with pressor doses. 6. Potassium and osmolal outputs were unchanged by non-pressor doses of vasopressin but significantly increased during administration of pressor doses. 7. It is concluded that pressor doses of lysine vasopressin do not alter total renal perfusion in conscious rats when the prostaglandin system is intact. Glomerular filtration is, however, decreased in a dose-dependent manner by these amounts but the mechanism is unclear.

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Selected References

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  1. Atherton J. C., Green R., Thomas S. Influence of lysine-vasopressin dosage on the time course of changes in renal tissue and urinary composition in the conscious rat. J Physiol. 1971 Mar;213(2):291–309. doi: 10.1113/jphysiol.1971.sp009383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atherton J. C., Hai M. A., Thomas S. Effects of water diuresis and osmotic (mannitol) diuresis on urinary solute excretion by the conscious rat. J Physiol. 1968 Jul;197(2):395–410. doi: 10.1113/jphysiol.1968.sp008566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Azzawi S. A., Shirley D. G. The effect of vasopressin on renal blood flow and its distribution in the rat. J Physiol. 1983 Aug;341:233–244. doi: 10.1113/jphysiol.1983.sp014803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baer P. G., McGiff J. C. Comparison of effects of prostaglandins E2 and I2 on rat renal vascular resistance. Eur J Pharmacol. 1979 Mar 15;54(4):359–363. doi: 10.1016/0014-2999(79)90065-7. [DOI] [PubMed] [Google Scholar]
  5. Balment R. J., Brimble M. J., Forsling M. L., Musabayane C. T. Natriuretic response of the rat to plasma concentrations of arginine vasopressin within the physiological range. J Physiol. 1984 Jul;352:517–526. doi: 10.1113/jphysiol.1984.sp015307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Beal A. M., Harrison F. A. Renal function in sheep during infusion of alkali metal ions into the renal artery. J Physiol. 1975 Feb;245(1):137–162. doi: 10.1113/jphysiol.1975.sp010838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cowley A. W., Jr, Barber B. J. Vasopressin vascular and reflex effects--a theoretical analysis. Prog Brain Res. 1983;60:415–424. doi: 10.1016/S0079-6123(08)64408-8. [DOI] [PubMed] [Google Scholar]
  8. Dolphin A. C., Forda S. R., Scott R. H. Calcium-dependent currents in cultured rat dorsal root ganglion neurones are inhibited by an adenosine analogue. J Physiol. 1986 Apr;373:47–61. doi: 10.1113/jphysiol.1986.sp016034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fisher R. D., Grünfeld J. P., Barger A. C. Intrarenal distribution of blood flow in diabetes insipidus: role of ADH. Am J Physiol. 1970 Nov;219(5):1348–1358. doi: 10.1152/ajplegacy.1970.219.5.1348. [DOI] [PubMed] [Google Scholar]
  10. Gerber J. G., Nies A. S. The hemodynamic effects of prostaglandins in the rat. Evidence for important species variation in renovascular responses. Circ Res. 1979 Mar;44(3):406–410. doi: 10.1161/01.res.44.3.406. [DOI] [PubMed] [Google Scholar]
  11. Gitelman H. J., Klapper D. G., Alderman F. R., Blythe W. B. Ala-Gly- and Val-Asp-[Arg8]-vasopressin: bovine storage forms of arginine vasopressin with natriuretic activity. Science. 1980 Feb 22;207(4433):893–896. doi: 10.1126/science.7355269. [DOI] [PubMed] [Google Scholar]
  12. Haylor J., Lote C. J. Renal function in conscious rats after indomethacin. Evidence for a tubular action of endogenous prostaglandins. J Physiol. 1980 Jan;298:371–381. doi: 10.1113/jphysiol.1980.sp013087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Haylor J., Towers J. Renal vasodilator activity of prostaglandin E2 in the rat anaesthetized with pentobarbitone. Br J Pharmacol. 1982 May;76(1):131–137. doi: 10.1111/j.1476-5381.1982.tb09198.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Heyndrickx G. R., Boettcher D. H., Vatner S. F. Effects of angiotensin, vasopressin, and methoxamine on cardiac function and blood flow distribution in conscious dogs. Am J Physiol. 1976 Nov;231(5 Pt 1):1579–1587. doi: 10.1152/ajplegacy.1976.231.5.1579. [DOI] [PubMed] [Google Scholar]
  15. Hof R. P. Modification of vasopressin- and angiotensin II- induced changes by calcium antagonists in the peripheral circulation of anaesthetized rabbits. Br J Pharmacol. 1985 May;85(1):75–87. doi: 10.1111/j.1476-5381.1985.tb08833.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Humphreys M. H., Friedler R. M., Earley L. E. Natrriuresis produced by vasopressin or hemorrhage during water diuresis in the dog. Am J Physiol. 1970 Sep;219(3):658–665. doi: 10.1152/ajplegacy.1970.219.3.658. [DOI] [PubMed] [Google Scholar]
  17. Ichikawa I., Brenner B. M. Evidence for glomerular actions of ADH and dibutyryl cyclic AMP in the rat. Am J Physiol. 1977 Aug;233(2):F102–F117. doi: 10.1152/ajprenal.1977.233.2.F102. [DOI] [PubMed] [Google Scholar]
  18. Jamison R. L., Maffly R. H. The urinary concentrating mechanism. N Engl J Med. 1976 Nov 4;295(19):1059–1067. doi: 10.1056/NEJM197611042951908. [DOI] [PubMed] [Google Scholar]
  19. Johnston C. I., Newman M., Woods R. Role of vasopressin in cardiovascular homeostasis and hypertension. Clin Sci (Lond) 1981 Dec;61 (Suppl 7):129s–139s. doi: 10.1042/cs061129s. [DOI] [PubMed] [Google Scholar]
  20. Kramp R. A., MacDowell M., Gottschalk C. W., Oliver J. R. A study by microdissection and micropuncture of the structure and the function of the kidneys and the nephrons of rats with chronic renal damage. Kidney Int. 1974 Feb;5(2):147–176. doi: 10.1038/ki.1974.19. [DOI] [PubMed] [Google Scholar]
  21. Lote C. J., McVicar A. J., Smyth D. G. Effects of vasopressin-glycine and vasopressin-glycine-lysine-arginine on renal function in the rat. J Endocrinol. 1986 Feb;108(2):255–260. doi: 10.1677/joe.0.1080255. [DOI] [PubMed] [Google Scholar]
  22. Lote C. J., McVicar A. J., Yardley C. P. Renal extraction and clearance of p-aminohippurate during saline and dextrose infusion in the rat. J Physiol. 1985 Jun;363:303–313. doi: 10.1113/jphysiol.1985.sp015711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lote C. J. Renal prostaglandins and sodium excretion. Q J Exp Physiol. 1982 Jul;67(3):377–385. doi: 10.1113/expphysiol.1982.sp002652. [DOI] [PubMed] [Google Scholar]
  24. Lote C. J., Snape B. M. Collecting duct dlow rate as a determinant of equilibration between urine and renal papilla in the rat in the presence of a maximal antidiuretic hormone concentration. J Physiol. 1977 Sep;270(2):533–544. doi: 10.1113/jphysiol.1977.sp011967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Montani J. P., Liard J. F., Schoun J., Möhring J. Hemodynamic effects of exogenous and endogenous vasopressin at low plasma concentrations in conscious dogs. Circ Res. 1980 Sep;47(3):346–355. doi: 10.1161/01.res.47.3.346. [DOI] [PubMed] [Google Scholar]
  26. Morgan T., Berliner R. W. A study by continuous microperfusion of water and electrolyte movements in the loop of Henle and distal tubule of the rat. Nephron. 1969;6(3):388–405. doi: 10.1159/000179741. [DOI] [PubMed] [Google Scholar]
  27. Oliver J. A., Sciacca R. R., Le Cren G., Cannon P. J. Modulation by prostaglandins of the renal vascular action of arginine vasopressin. Prostaglandins. 1982 Nov;24(5):641–656. doi: 10.1016/0090-6980(82)90034-x. [DOI] [PubMed] [Google Scholar]
  28. Schmid P. G., Abboud F. M., Wendling M. G., Ramberg E. S., Mark A. L., Heistad D. D., Eckstein J. W. Regional vascular effects of vasopressin: plasma levels and circulatory responses. Am J Physiol. 1974 Nov;227(5):998–1004. doi: 10.1152/ajplegacy.1974.227.5.998. [DOI] [PubMed] [Google Scholar]
  29. Smith H. W., Finkelstein N., Aliminosa L., Crawford B., Graber M. THE RENAL CLEARANCES OF SUBSTITUTED HIPPURIC ACID DERIVATIVES AND OTHER AROMATIC ACIDS IN DOG AND MAN. J Clin Invest. 1945 May;24(3):388–404. doi: 10.1172/JCI101618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Terragno N. A., Terragno D. A., McGiff J. C. Contribution of prostaglandins to the renal circulation in conscious, anesthetized, and laparotomized dogs. Circ Res. 1977 Jun;40(6):590–595. doi: 10.1161/01.res.40.6.590. [DOI] [PubMed] [Google Scholar]
  31. VAN DYKE H. B., ENGEL S. L., ADAMSONS K., Jr Comparison of pharmacological effects of lysine and arginine vasopressins. Proc Soc Exp Biol Med. 1956 Mar;91(3):484–486. doi: 10.3181/00379727-91-22300. [DOI] [PubMed] [Google Scholar]

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