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. 1987 Jun;387:1–17. doi: 10.1113/jphysiol.1987.sp016558

Vasoactive intestinal polypeptide regulation of rabbit renal adenylate cyclase activity in vitro.

N M Griffiths 1, N L Simmons 1
PMCID: PMC1192489  PMID: 3656172

Abstract

1. The effect of vasoactive intestinal polypeptide (VIP) upon adenylate cyclase activity was determined in purified cortical basolateral membranes and in glomeruli and tubular elements obtained from rabbit kidney. 2. In purified basolateral membranes prepared from cortex, 1 microM-VIP consistently stimulated adenylate cyclase activity above basal levels (1.55 +/- 0.09-fold (mean +/- S.E. of mean), n = 10 animals). Half-maximal stimulation was observed at 17 +/- 11 nM-VIP (S.D., n = 9). 3. Related peptides, e.g. secretin, glucagon, gastric inhibitory peptide, human pancreatic growth hormone releasing factor, and peptide having N-terminal histidine and C-terminal isoleucine amide (PHI), were without effect or gave lower stimulations of adenylate cyclase activity when tested at 1 microM. 4. Significant VIP degradation was observed under the assay conditions used but this did not substantially alter the response or selectivity to VIP. 5. In separate preparations of isolated glomeruli and proximal tubules addition of 1 microM-VIP resulted in a 3.3 +/- 1.1-fold (S.D., n = 3) and 2.2 +/- 1.0-fold (S.D., n = 3) stimulation (respectively) of adenylate cyclase activity. 6. In isolated medullary tubule suspensions, isolated by collagenase-hyaluronidase digestion of outer (red) medulla, and in thick ascending-limb-enriched preparations prepared by Percoll density gradient fractionation, 1 microM-VIP significantly increased adenylate cyclase activity by 2.4 +/- 0.6-fold (S.D., n = 3) and 2.1 +/- 0.7-fold (S.D., n = 3) respectively. 7. A possible role for VIP in the regulation of renal function in the rabbit is discussed in relation to the occurrence of VIP stimulation of adenylate cyclase activity in several renal cellular elements.

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Selected References

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  1. Bailly C., Imbert-Teboul M., Chabardès D., Hus-Citharel A., Montégut M., Clique A., Morel F. The distal nephron of rat kidney: a target site for glucagon. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3422–3424. doi: 10.1073/pnas.77.6.3422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barajas L., Sokolski K. N., Lechago J. Vasoactive intestinal polypeptide-immunoreactive nerves in the kidney. Neurosci Lett. 1983 Dec 30;43(2-3):263–269. doi: 10.1016/0304-3940(83)90199-4. [DOI] [PubMed] [Google Scholar]
  3. Bataille D., Gespach C., Laburthe M., Amiranoff B., Tatemoto K., Vauclin N., Mutt V., Rosselin G. Porcine peptide having N-terminal histidine and C-terminal isoleucine amide (PHI): vasoactive intestinal peptide (VIP) and secretin-like effects in different tissues from the rat. FEBS Lett. 1980 Jun 2;114(2):240–242. doi: 10.1016/0014-5793(80)81124-0. [DOI] [PubMed] [Google Scholar]
  4. Boumendil-Podevin E. F., Podevin R. A. Isolation of basolateral and brush-border membranes from the rabbit kidney cortex. Vesicle integrity and membrane sidedness of the basolateral fraction. Biochim Biophys Acta. 1983 Oct 26;735(1):86–94. doi: 10.1016/0005-2736(83)90263-8. [DOI] [PubMed] [Google Scholar]
  5. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  6. Brown B. L., Albano J. D., Ekins R. P., Sgherzi A. M. A simple and sensitive saturation assay method for the measurement of adenosine 3':5'-cyclic monophosphate. Biochem J. 1971 Feb;121(3):561–562. doi: 10.1042/bj1210561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. COOK W. F., PICKERING G. W. A rapid method for separating glomeruli from rabbit kidney. Nature. 1958 Oct 18;182(4642):1103–1104. doi: 10.1038/1821103a0. [DOI] [PubMed] [Google Scholar]
  8. Calam J., Dimaline R., Peart W. S., Singh J., Unwin R. J. Effects of vasoactive intestinal polypeptide on renal function in man. J Physiol. 1983 Dec;345:469–475. doi: 10.1113/jphysiol.1983.sp014989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chamberlin M. E., LeFurgey A., Mandel L. J. Suspension of medullary thick ascending limb tubules from the rabbit kidney. Am J Physiol. 1984 Dec;247(6 Pt 2):F955–F964. doi: 10.1152/ajprenal.1984.247.6.F955. [DOI] [PubMed] [Google Scholar]
  10. Chung S. D., Alavi N., Livingston D., Hiller S., Taub M. Characterization of primary rabbit kidney cultures that express proximal tubule functions in a hormonally defined medium. J Cell Biol. 1982 Oct;95(1):118–126. doi: 10.1083/jcb.95.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Couvineau A., Laburthe M. The rat liver vasoactive intestinal peptide binding site. Molecular characterization by covalent cross-linking and evidence for differences from the intestinal receptor. Biochem J. 1985 Jan 15;225(2):473–479. doi: 10.1042/bj2250473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dharmsathaphorn K., Harms V., Yamashiro D. J., Hughes R. J., Binder H. J., Wright E. M. Preferential binding of vasoactive intestinal polypeptide to basolateral membrane of rat and rabbit enterocytes. J Clin Invest. 1983 Jan;71(1):27–35. doi: 10.1172/JCI110748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. DiBona G. F. Neural regulation of renal tubular sodium reabsorption and renin secretion. Fed Proc. 1985 Oct;44(13):2816–2822. [PubMed] [Google Scholar]
  14. Dimaline R., Peart W. S., Unwin R. J. Effects of vasoactive intestinal polypeptide (VIP) on renal function and plasma renin activity in the conscious rabbit. J Physiol. 1983 Nov;344:379–388. doi: 10.1113/jphysiol.1983.sp014946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Dousa T. P., Shah S. V., Abboud H. E. Potential role of cyclic nucleotides in glomerular pathophysiology. Adv Cyclic Nucleotide Res. 1980;12:285–299. [PubMed] [Google Scholar]
  16. Greger R. Ion transport mechanisms in thick ascending limb of Henle's loop of mammalian nephron. Physiol Rev. 1985 Jul;65(3):760–797. doi: 10.1152/physrev.1985.65.3.760. [DOI] [PubMed] [Google Scholar]
  17. Keltz T. N., Straus E., Yalow R. S. Degradation of vasoactive intestinal polypeptide by tissue homogenates. Biochem Biophys Res Commun. 1980 Jan 29;92(2):669–674. doi: 10.1016/0006-291x(80)90385-x. [DOI] [PubMed] [Google Scholar]
  18. Kopp U. C. Renorenal reflexes: neural and functional responses. Fed Proc. 1985 Oct;44(13):2834–2839. [PubMed] [Google Scholar]
  19. Pandol S. J., Dharmsathaphorn K., Schoeffield M. S., Vale W., Rivier J. Vasoactive intestinal peptide receptor antagonist [4Cl-D-Phe6, Leu17] VIP. Am J Physiol. 1986 Apr;250(4 Pt 1):G553–G557. doi: 10.1152/ajpgi.1986.250.4.G553. [DOI] [PubMed] [Google Scholar]
  20. Porter J. P., Said S. I., Ganong W. F. Vasoactive intestinal peptide stimulates renin secretion in vitro: evidence for a direct action of the peptide on the renal juxtaglomerular cells. Neuroendocrinology. 1983 May;36(5):404–408. doi: 10.1159/000123488. [DOI] [PubMed] [Google Scholar]
  21. Porter J. P., Thrasher T. N., Said S. I., Ganong W. F. Vasoactive intestinal peptide in the regulation of renin secretion. Am J Physiol. 1985 Jul;249(1 Pt 2):F84–F89. doi: 10.1152/ajprenal.1985.249.1.F84. [DOI] [PubMed] [Google Scholar]
  22. Rosa R. M., Silva P., Stoff J. S., Epstein F. H. Effect of vasoactive intestinal peptide on isolated perfused rat kidney. Am J Physiol. 1985 Nov;249(5 Pt 1):E494–E497. doi: 10.1152/ajpendo.1985.249.5.E494. [DOI] [PubMed] [Google Scholar]
  23. Rugg E. L., Simmons N. L. Control of cultured epithelial (MDCK) cell transport function: identification of a beta-adrenoceptor coupled to adenylate cyclase. Q J Exp Physiol. 1984 Apr;69(2):339–353. doi: 10.1113/expphysiol.1984.sp002810. [DOI] [PubMed] [Google Scholar]
  24. Schwartz C. J., Kimberg D. V., Sheerin H. E., Field M., Said S. I. Vasoactive intestinal peptide stimulation of adenylate cyclase and active electrolyte secretion in intestinal mucosa. J Clin Invest. 1974 Sep;54(3):536–544. doi: 10.1172/JCI107790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Simmons N. L., Brown C. D., Rugg E. L. The action of epinephrine on Madin-Darby canine kidney cells. Fed Proc. 1984 May 15;43(8):2225–2229. [PubMed] [Google Scholar]
  26. Sueiras-Diaz J., Lance V. A., Murphy W. A., Coy D. H. Structure-activity studies on the N-terminal region of glucagon. J Med Chem. 1984 Mar;27(3):310–315. doi: 10.1021/jm00369a014. [DOI] [PubMed] [Google Scholar]
  27. Waelbroeck M., Robberecht P., Coy D. H., Camus J. C., De Neef P., Christophe J. Interaction of growth hormone-releasing factor (GRF) and 14 GRF analogs with vasoactive intestinal peptide (VIP) receptors of rat pancreas. Discovery of (N-Ac-Tyr1,D-Phe2)-GRF(1-29)-NH2 as a VIP antagonist. Endocrinology. 1985 Jun;116(6):2643–2649. doi: 10.1210/endo-116-6-2643. [DOI] [PubMed] [Google Scholar]

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