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. 1986 Feb;371:201–217. doi: 10.1113/jphysiol.1986.sp015969

Changes by acetylcholine of membrane currents in rabbit cardiac Purkinje fibres.

E Carmeliet, K Mubagwa
PMCID: PMC1192718  PMID: 2422347

Abstract

The ionic mechanisms underlying the effects of acetylcholine (ACh) on electrophysiological properties of rabbit cardiac Purkinje fibres have been analysed using the two-micro-electrode voltage-clamp technique on short preparations. In normal Tyrode solution, ACh shifted the membrane currents in the outward direction at potentials positive to the K+ equilibrium potential, EK, and in the inward direction at potentials negative to EK. When ACh effects were studied in various [K+]o the reversal potential for the ACh-induced current was changed in a way expected for a pure K+ electrode. The results indicate an increase of an inward-rectifying K+ conductance by ACh. ACh also decreased the magnitude of the late outward current (ix). The activation curve for this current was not modified, but the fully activated current was decreased. The effect of ACh on ix was more pronounced in the presence of catecholamines. The slow inward current (isi) was not changed by ACh in control conditions. However, the increase produced by beta-adrenergic stimulation in this current was suppressed by ACh in a concentration-dependent way.

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Selected References

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  1. Argibay J. A., Dutey P., Ildefonse M., Ojeda C., Rougier O., Tourneur Y. Block by Cs of K current iK1 and of carbachol induced K current iCch in frog atrium. Pflugers Arch. 1983 Jun 1;397(4):295–299. doi: 10.1007/BF00580264. [DOI] [PubMed] [Google Scholar]
  2. Aronson R. S., Gelles J. M., Hoffman B. F. A new method for producing short cardiac Purkinje fibers suitable for voltage clamp. J Appl Physiol. 1973 Apr;34(4):527–530. doi: 10.1152/jappl.1973.34.4.527. [DOI] [PubMed] [Google Scholar]
  3. Attwell D., Cohen I., Eisner D., Ohba M., Ojeda C. The steady state TTX-sensitive ("window") sodium current in cardiac Purkinje fibres. Pflugers Arch. 1979 Mar 16;379(2):137–142. doi: 10.1007/BF00586939. [DOI] [PubMed] [Google Scholar]
  4. Bailey J. C., Watanabe A. M., Besch H. R., Jr, Lathrop D. A. Acetylcholine antagonism of the electrophysiological effects of isoproterenol on canine cardiac Purkinje fibers. Circ Res. 1979 Mar;44(3):378–383. doi: 10.1161/01.res.44.3.378. [DOI] [PubMed] [Google Scholar]
  5. Biegon R. L., Pappano A. J. Dual mechanism for inhibition of calcium-dependent action potentials by acetylcholine in avian ventricular muscle. Relationship to cyclic AMP. Circ Res. 1980 Mar;46(3):353–362. doi: 10.1161/01.res.46.3.353. [DOI] [PubMed] [Google Scholar]
  6. Carmeliet E., Mubagwa K. Characterization of the acetylcholine-induced potassium current in rabbit cardiac Purkinje fibres. J Physiol. 1986 Feb;371:219–237. doi: 10.1113/jphysiol.1986.sp015970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carmeliet E., Mubagwa K. Desensitization of the acetylcholine-induced increase of potassium conductance in rabbit cardiac Purkinje fibres. J Physiol. 1986 Feb;371:239–255. doi: 10.1113/jphysiol.1986.sp015971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Carmeliet E., Ramon J. Effect of acetylcholine on time-independent currents in sheep cardiac Purkinje fibers. Pflugers Arch. 1980 Sep;387(3):207–216. doi: 10.1007/BF00580972. [DOI] [PubMed] [Google Scholar]
  9. Carmeliet E., Ramon J. Effects of acetylcholine on time-dependent currents in sheep cardiac Purkinje fibers. Pflugers Arch. 1980 Sep;387(3):217–223. doi: 10.1007/BF00580973. [DOI] [PubMed] [Google Scholar]
  10. Carmeliet E., Ramon J. Electrophysiological effects of acetylcholine in sheep cardiac Purkinje fibers. Pflugers Arch. 1980 Sep;387(3):197–205. doi: 10.1007/BF00580971. [DOI] [PubMed] [Google Scholar]
  11. Carmeliet E., Saikawa T. Shortening of the action potential and reduction of pacemaker activity by lidocaine, quinidine, and procainamide in sheep cardiac purkinje fibers. An effect on Na or K currents? Circ Res. 1982 Feb;50(2):257–272. doi: 10.1161/01.res.50.2.257. [DOI] [PubMed] [Google Scholar]
  12. Colatsky T. J., Tsien R. W. Electrical properties associated with wide intercellular clefts in rabbit Purkinje fibres. J Physiol. 1979 May;290(2):227–252. doi: 10.1113/jphysiol.1979.sp012769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Coraboeuf E., Deroubaix E., Coulombe A. Effect of tetrodotoxin on action potentials of the conducting system in the dog heart. Am J Physiol. 1979 Apr;236(4):H561–H567. doi: 10.1152/ajpheart.1979.236.4.H561. [DOI] [PubMed] [Google Scholar]
  14. DiFrancesco D. A new interpretation of the pace-maker current in calf Purkinje fibres. J Physiol. 1981 May;314:359–376. doi: 10.1113/jphysiol.1981.sp013713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gadsby D. C., Wit A. L., Cranefield P. F. The effects of acetylcholine on the electrical activity of canine cardiac Purkinje fibers. Circ Res. 1978 Jul;43(1):29–35. doi: 10.1161/01.res.43.1.29. [DOI] [PubMed] [Google Scholar]
  16. Garnier D., Nargeot J., Ojeda C., Rougier O. The action of acetylcholine on background conductance in frog atrial trabeculae. J Physiol. 1978 Jan;274:381–396. doi: 10.1113/jphysiol.1978.sp012154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Giles W., Noble S. J. Changes in membrane currents in bullfrog atrium produced by acetylcholine. J Physiol. 1976 Sep;261(1):103–123. doi: 10.1113/jphysiol.1976.sp011550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hauswirth O., Noble D., Tsien R. W. Separation of the pace-maker and plateau components of delayed rectification in cardiac Purkinje fibres. J Physiol. 1972 Aug;225(1):211–235. doi: 10.1113/jphysiol.1972.sp009934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Higgins C. B., Vatner S. F., Braunwald E. Parasympathetic control of the heart. Pharmacol Rev. 1973 Mar;25(1):119–155. [PubMed] [Google Scholar]
  20. Hill-Smith I., Purves R. D. Synaptic delay in the heart: an ionophoretic study. J Physiol. 1978 Jun;279:31–54. doi: 10.1113/jphysiol.1978.sp012329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Hino N., Ochi R. Effect of acetylcholine on membrane currents in guinea-pig papillary muscle. J Physiol. 1980 Oct;307:183–197. doi: 10.1113/jphysiol.1980.sp013430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ikemoto Y., Goto M. Effects of ACh on slow inward current and tension components of the bullfrog atrium. J Mol Cell Cardiol. 1977 Apr;9(4):313–326. doi: 10.1016/s0022-2828(77)80037-0. [DOI] [PubMed] [Google Scholar]
  23. Josephson I., Sperelakis N. On the ionic mechanism underlying adrenergic-cholinergic antagonism in ventricular muscle. J Gen Physiol. 1982 Jan;79(1):69–86. doi: 10.1085/jgp.79.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Kass R. S., Tsien R. W. Multiple effects of calcium antagonists on plateau currents in cardiac Purkinje fibers. J Gen Physiol. 1975 Aug;66(2):169–192. doi: 10.1085/jgp.66.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Lee C. O., Fozzard H. A. Activities of potassium and sodium ions in rabbit heart muscle. J Gen Physiol. 1975 Jun;65(6):695–708. doi: 10.1085/jgp.65.6.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Lipsius S. L., Gibbons W. R. Acetylcholine lengthens action potentials of sheep cardiac Purkinje fibers. Am J Physiol. 1980 Feb;238(2):H237–H243. doi: 10.1152/ajpheart.1980.238.2.H237. [DOI] [PubMed] [Google Scholar]
  27. Miura D. S., Hoffman B. F., Rosen M. R. The effect of extracellular potassium on the intracellular potassium ion activity and transmembrane potentials of beating canine cardiac Purkinje fibers. J Gen Physiol. 1977 Apr;69(4):463–474. doi: 10.1085/jgp.69.4.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Mubagwa K., Carmeliet E. Effects of acetylcholine on electrophysiological properties of rabbit cardiac Purkinje fibers. Circ Res. 1983 Dec;53(6):740–751. doi: 10.1161/01.res.53.6.740. [DOI] [PubMed] [Google Scholar]
  29. Noble D., Tsien R. W. Outward membrane currents activated in the plateau range of potentials in cardiac Purkinje fibres. J Physiol. 1969 Jan;200(1):205–231. doi: 10.1113/jphysiol.1969.sp008689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Noma A., Trautwein W. Relaxation of the ACh-induced potassium current in the rabbit sinoatrial node cell. Pflugers Arch. 1978 Nov 30;377(3):193–200. doi: 10.1007/BF00584272. [DOI] [PubMed] [Google Scholar]
  31. Ojeda C., Rougier O., Tourneur Y. Effects of Cs on acetylcholine induced current. Is ik1 increased by acetylcholine in frog atrium? Pflugers Arch. 1981 Jul;391(1):57–59. doi: 10.1007/BF00580695. [DOI] [PubMed] [Google Scholar]
  32. Osterrieder W., Yang Q. F., Trautwein W. The time course of the muscarinic response to ionophoretic acetylcholine application to the S-A node of the rabbit heart. Pflugers Arch. 1981 Mar;389(3):283–291. doi: 10.1007/BF00584791. [DOI] [PubMed] [Google Scholar]
  33. Sakmann B., Noma A., Trautwein W. Acetylcholine activation of single muscarinic K+ channels in isolated pacemaker cells of the mammalian heart. Nature. 1983 May 19;303(5914):250–253. doi: 10.1038/303250a0. [DOI] [PubMed] [Google Scholar]
  34. Sheu S. S., Korth M., Lathrop D. A., Fozzard H. A. Intra- and extracellular K+ and Na+ activities and resting membrane potential in sheep cardiac purkinje strands. Circ Res. 1980 Nov;47(5):692–700. doi: 10.1161/01.res.47.5.692. [DOI] [PubMed] [Google Scholar]
  35. Soejima M., Noma A. Mode of regulation of the ACh-sensitive K-channel by the muscarinic receptor in rabbit atrial cells. Pflugers Arch. 1984 Apr;400(4):424–431. doi: 10.1007/BF00587544. [DOI] [PubMed] [Google Scholar]
  36. Sommer J. R., Johnson E. A. Cardiac muscle. A comparative study of Purkinje fibers and ventricular fibers. J Cell Biol. 1968 Mar;36(3):497–526. doi: 10.1083/jcb.36.3.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. TRAUTWEIN W., DUDEL J. Zum Mechanismus der Membranwirkung des Acetylcholin an der Herzmuskelfaser. Pflugers Arch. 1958;266(3):324–334. doi: 10.1007/BF00416781. [DOI] [PubMed] [Google Scholar]
  38. Ten Eick R., Nawrath H., McDonald T. F., Trautwein W. On the mechanism of the negative inotropic effect of acetylcholine. Pflugers Arch. 1976 Feb 24;361(3):207–213. doi: 10.1007/BF00587284. [DOI] [PubMed] [Google Scholar]
  39. Watanabe A. M., Besch H. R., Jr Interaction between cyclic adenosine monophosphate and cyclic gunaosine monophosphate in guinea pig ventricular myocardium. Circ Res. 1975 Sep;37(3):309–317. doi: 10.1161/01.res.37.3.309. [DOI] [PubMed] [Google Scholar]

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