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. 1984 Nov;356:375–390. doi: 10.1113/jphysiol.1984.sp015471

Analysis of 'off' tails of intramembrane charge movements in skeletal muscle of Rana temporaria.

C L Huang
PMCID: PMC1193170  PMID: 6335176

Abstract

Components of non-linear charge were isolated in transients obtained in response to hyperpolarizing ('off') voltage-clamp steps in frog muscle fibres. 'Off' currents of the q gamma charge were isolated by comparing records obtained from long depolarizing steps with those resulting from short steps which intercepted the 'on' currents of the q gamma charge. The 'off' transients of the q gamma component so deduced were rapid decays lasting 10-15 ms, in contrast with their prolonged time course in the preceding 'on' steps. 'Off' responses were rapid even at voltages when the 'on' current, obtained from imposed 10 mV steps made at a series of closely incremented conditioning voltages, was delayed and prolonged. Such slow transfers of charge could not be demonstrated in 'off' tails. Large depolarizing steps resulted in rapid q gamma transients in 'on' currents not distinguishable from the rest of the charge movement. Nevertheless, by separating the q gamma component through its inactivation by prolonged depolarization, it was possible to show that q gamma currents in the 'off' tails were still rapid decays. It is concluded that in contrast to the varied pattern shown by q gamma in 'on' transients, its 'off' responses are everywhere relatively fast decays. These features can be predicted by a simple two-state model in which the forward and backward rate constants depend upon the amount of charge moved, as well as the membrane voltage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adrian R. H., Almers W. Membrane capacity measurements on frog skeletal muscle in media of low ion content. J Physiol. 1974 Mar;237(3):573–605. doi: 10.1113/jphysiol.1974.sp010499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adrian R. H. Charge movement in the membrane of striated muscle. Annu Rev Biophys Bioeng. 1978;7:85–112. doi: 10.1146/annurev.bb.07.060178.000505. [DOI] [PubMed] [Google Scholar]
  3. Adrian R. H., Huang C. L. Charge movements near the mechanical threshold in skeletal muscle of Rana temporaria. J Physiol. 1984 Apr;349:483–500. doi: 10.1113/jphysiol.1984.sp015169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Adrian R. H., Huang C. L. Experimental analysis of the relationship between charge movement components in skeletal muscle of Rana temporaria. J Physiol. 1984 Aug;353:419–434. doi: 10.1113/jphysiol.1984.sp015344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Adrian R. H., Peachey L. D. Reconstruction of the action potential of frog sartorius muscle. J Physiol. 1973 Nov;235(1):103–131. doi: 10.1113/jphysiol.1973.sp010380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Adrian R. H., Peres A. R. A gating signal for the potassium channel? Nature. 1977 Jun 30;267(5614):800–804. doi: 10.1038/267800a0. [DOI] [PubMed] [Google Scholar]
  7. Adrian R. H., Peres A. Charge movement and membrane capacity in frog muscle. J Physiol. 1979 Apr;289:83–97. doi: 10.1113/jphysiol.1979.sp012726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Adrian R. H., Rakowski R. F. Reactivation of membrane charge movement and delayed potassium conductance in skeletal muscle fibres. J Physiol. 1978 May;278:533–557. doi: 10.1113/jphysiol.1978.sp012323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Anderson W. P., Korner P. I. The importance of renal vascular tone in determining the severity of renal artery stenosis in dogs. J Physiol. 1980 Aug;305:31–41. doi: 10.1113/jphysiol.1980.sp013347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chandler W. K., Rakowski R. F., Schneider M. F. A non-linear voltage dependent charge movement in frog skeletal muscle. J Physiol. 1976 Jan;254(2):245–283. doi: 10.1113/jphysiol.1976.sp011232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Duane S., Huang C. L. A quantitative description of the voltage-dependent capacitance in frog skeletal muscle in terms of equilibrium statistical mechanics. Proc R Soc Lond B Biol Sci. 1982 Apr 22;215(1198):75–94. doi: 10.1098/rspb.1982.0029. [DOI] [PubMed] [Google Scholar]
  12. HODGKIN A. L., HUXLEY A. F. A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol. 1952 Aug;117(4):500–544. doi: 10.1113/jphysiol.1952.sp004764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hodgkin A. L., Nakajima S. The effect of diameter on the electrical constants of frog skeletal muscle fibres. J Physiol. 1972 Feb;221(1):105–120. doi: 10.1113/jphysiol.1972.sp009742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Horowicz P., Schneider M. F. Membrane charge movement in contracting and non-contracting skeletal muscle fibres. J Physiol. 1981 May;314:565–593. doi: 10.1113/jphysiol.1981.sp013725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Huang C. L. Dielectric components of charge movements in skeletal muscle. J Physiol. 1981;313:187–205. doi: 10.1113/jphysiol.1981.sp013658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Huang C. L. Effects of local anaesthetics on the relationship between charge movements and contractile thresholds in frog skeletal muscle. J Physiol. 1981 Nov;320:381–391. doi: 10.1113/jphysiol.1981.sp013956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Huang C. L. Experimental analysis of alternative models of charge movement in frog skeletal muscle. J Physiol. 1983 Mar;336:527–543. doi: 10.1113/jphysiol.1983.sp014596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Huang C. L. Pharmacological separation of charge movement components in frog skeletal muscle. J Physiol. 1982 Mar;324:375–387. doi: 10.1113/jphysiol.1982.sp014118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Huang C. L. Time domain spectroscopy of the membrane capacitance in frog skeletal muscle. J Physiol. 1983 Aug;341:1–24. doi: 10.1113/jphysiol.1983.sp014789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hui C. S. Pharmacological studies of charge movement in frog skeletal muscle. J Physiol. 1983 Apr;337:509–529. doi: 10.1113/jphysiol.1983.sp014639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schneider M. F., Chandler W. K. Voltage dependent charge movement of skeletal muscle: a possible step in excitation-contraction coupling. Nature. 1973 Mar 23;242(5395):244–246. doi: 10.1038/242244a0. [DOI] [PubMed] [Google Scholar]

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