Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1983 Dec;345:525–532. doi: 10.1113/jphysiol.1983.sp014994

Energy metabolism and contraction force of human skeletal muscle in situ during electrical stimulation.

E Hultman, H Sjöholm
PMCID: PMC1193813  PMID: 6663511

Abstract

The quadriceps femoris muscles of nine volunteers were stimulated with intramuscular electrodes for 50 s. The stimulation frequency was 20 Hz and the voltage adjusted to produce an initial tension of 50-75% of the maximum voluntary contraction force. The force decreased after 25-30 s of contraction to reach a mean of 78% of the initial value after 50 s. During the stimulation up to four muscle biopsy samples were taken from each leg. The ATP turnover rate was initially 5.6 mmol kg-1 dry wt. s-1 and decreased during the later stages of contraction to 4.0 mmol kg-1 dry wt. s-1. The force declined at approximately the same rate as the ATP turnover during the contraction. The phosphocreatine (PCr) store decreased exponentially during contraction and was practically depleted at 50 s. Glycolysis began within 5 s after initiation of contraction. The rate of glycolysis measured as lactate accumulation increased with time throughout the entire contraction period.

Full text

PDF
525

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergström J., Guarnieri G., Hultman E. Carbohydrate metabolism and electrolyte changes in human muscle tissue during heavy work. J Appl Physiol. 1971 Jan;30(1):122–125. doi: 10.1152/jappl.1971.30.1.122. [DOI] [PubMed] [Google Scholar]
  2. Chance B., Eleff S., Bank W., Leigh J. S., Jr, Warnell R. 31P NMR studies of control of mitochondrial function in phosphofructokinase-deficient human skeletal muscle. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7714–7718. doi: 10.1073/pnas.79.24.7714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dawson M. J., Gadian D. G., Wilkie D. R. Muscular fatigue investigated by phosphorus nuclear magnetic resonance. Nature. 1978 Aug 31;274(5674):861–866. doi: 10.1038/274861a0. [DOI] [PubMed] [Google Scholar]
  4. Edwards R. H., Dawson M. J., Wilkie D. R., Gordon R. E., Shaw D. Clinical use of nuclear magnetic resonance in the investigation of myopathy. Lancet. 1982 Mar 27;1(8274):725–731. doi: 10.1016/s0140-6736(82)92635-6. [DOI] [PubMed] [Google Scholar]
  5. Edwards R. H. Human muscle function and fatigue. Ciba Found Symp. 1981;82:1–18. doi: 10.1002/9780470715420.ch1. [DOI] [PubMed] [Google Scholar]
  6. Edwards R. H., Young A., Hosking G. P., Jones D. A. Human skeletal muscle function: description of tests and normal values. Clin Sci Mol Med. 1977 Mar;52(3):283–290. doi: 10.1042/cs0520283. [DOI] [PubMed] [Google Scholar]
  7. Harris R. C., Essén B., Hultman E. Glycogen phosphorylase activity in biopsy samples and single muscle fibres of musculus quadriceps femoris of man at rest. Scand J Clin Lab Invest. 1976 Oct;36(6):521–526. doi: 10.1080/00365517609054473. [DOI] [PubMed] [Google Scholar]
  8. Harris R. C., Hultman E., Kaijser L., Nordesjö L. O. The effect of circulatory occlusion on isometric exercise capacity and energy metabolism of the quadriceps muscle in man. Scand J Clin Lab Invest. 1975 Jan;35(1):87–95. [PubMed] [Google Scholar]
  9. Harris R. C., Hultman E., Nordesjö L. O. Glycogen, glycolytic intermediates and high-energy phosphates determined in biopsy samples of musculus quadriceps femoris of man at rest. Methods and variance of values. Scand J Clin Lab Invest. 1974 Apr;33(2):109–120. [PubMed] [Google Scholar]
  10. Harris R. C., Hultman E., Sahlin K. Glycolytic intermediates in human muscle after isometric contraction. Pflugers Arch. 1981 Mar;389(3):277–282. doi: 10.1007/BF00584790. [DOI] [PubMed] [Google Scholar]
  11. Hultman E., Sjöholm H., Sahlin K., Edström L. Glycolytic and oxidative energy metabolism and contraction characteristics of intact human muscle. Ciba Found Symp. 1981;82:19–40. [PubMed] [Google Scholar]
  12. MARGARIA R., CERRETELLI P., MANGILI F. BALANCE AND KINETICS OF ANAEROBIC ENERGY RELEASE DURING STRENUOUS EXERCISE IN MAN. J Appl Physiol. 1964 Jul;19:623–628. doi: 10.1152/jappl.1964.19.4.623. [DOI] [PubMed] [Google Scholar]
  13. Margaria R., Aghemo P., Rovelli E. Measurement of muscular power (anaerobic) in man. J Appl Physiol. 1966 Sep;21(5):1662–1664. doi: 10.1152/jappl.1966.21.5.1662. [DOI] [PubMed] [Google Scholar]
  14. Trivedi B., Danforth W. H. Effect of pH on the kinetics of frog muscle phosphofructokinase. J Biol Chem. 1966 Sep 10;241(17):4110–4112. [PubMed] [Google Scholar]
  15. Ui M. A role of phosphofructokinase in pH-dependent regulation of glycolysis. Biochim Biophys Acta. 1966 Aug 24;124(2):310–322. doi: 10.1016/0304-4165(66)90194-2. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES