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. 1983 Sep;342:499–515. doi: 10.1113/jphysiol.1983.sp014865

Electrical responses of smooth muscle cells of the mouse uterus to adenosine triphosphate.

J G Ninomiya, H Suzuki
PMCID: PMC1193973  PMID: 6631746

Abstract

Electrical responses of the smooth muscle cells to ATP were recorded in the longitudinal muscle of mouse myometrium, using intracellular micro-electrodes. ATP (greater than 10(-7) M) dose-dependently produced a biphasic change in the membrane potential, an initial hyperpolarization (20-30 sec) and then a depolarization. This effect of ATP was observed in all stages of gestation. The initial hyperpolarization was more quickly desensitized than the depolarization. Application of ATP for a short period (10 sec) produced only the initial hyperpolarization; the amplitude was dose-dependently increased. During the ATP-induced hyperpolarization and the depolarization, generation of spike potentials was suppressed and enhanced, respectively. Strong outward current restored the spike generation during hyperpolarization. During the ATP-induced hyperpolarization, the membrane resistance was decreased. The amplitude of the hyperpolarization was increased in low [K]0 solution and decreased in high [K]0 solutions. Pre-treatment with TEA (1 mM), procaine (1 mM), 4-aminopyridine (0.5 mM) or apamin (2 X 10(-7) M) did not, but TEA (5-10 mM) did suppress the ATP-induced hyperpolarization. Involvement of endogenous catecholamines, cyclic AMP, prostaglandins or acetylcholine in the ATP responses was ruled out. During the ATP-induced depolarization, the membrane resistance was reduced. In low [Na]0 solutions, the muscle membrane was depolarized and the amplitude of ATP-induced depolarization was reduced. In sodium-free solution, ATP produced only the initial hyperpolarization. It was concluded that the electrical responses of the smooth muscle cells of mouse myometrium to ATP consist of two components: an initial hyperpolarization with increase in the potassium conductance and a depolarization with increase in the sodium conductance.

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Selected References

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  1. Abe Y., Tomita T. Cable properties of smooth muscle. J Physiol. 1968 May;196(1):87–100. doi: 10.1113/jphysiol.1968.sp008496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson G. F., Kawarabayashi T., Marshall J. M. Effect of indomethacin and aspirin on uterine activity in pregnant rats: comparison of circular and longitudinal muscle. Biol Reprod. 1981 Mar;24(2):359–372. doi: 10.1095/biolreprod24.2.359. [DOI] [PubMed] [Google Scholar]
  3. Burnstock G., Cocks T., Paddle B., Staszewska-Barczak J. Evidence that prostaglandin is responsible for the 'rebound contraction' following stimulation of non-adrenergic, non-cholinergic ('purinergic') inhibitory nerves. Eur J Pharmacol. 1975 Apr;31(2):360–362. doi: 10.1016/0014-2999(75)90060-6. [DOI] [PubMed] [Google Scholar]
  4. Burnstock G. Purinergic nerves. Pharmacol Rev. 1972 Sep;24(3):509–581. [PubMed] [Google Scholar]
  5. Burnstock G. Purinergic receptors. J Theor Biol. 1976 Oct 21;62(2):491–503. doi: 10.1016/0022-5193(76)90133-8. [DOI] [PubMed] [Google Scholar]
  6. Burnstock G. Review lecture. Neurotransmitters and trophic factors in the autonomic nervous system. J Physiol. 1981;313:1–35. doi: 10.1113/jphysiol.1981.sp013648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bülbring E., Casteels R., Kuriyama H. Membrane potential and ion content in cat and guinea-pig myometrium and the response to adrenaline and noradrenaline. Br J Pharmacol. 1968 Oct;34(2):388–407. doi: 10.1111/j.1476-5381.1968.tb07060.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. CASTEELS R., KURIYAMA H. MEMBRANE POTENTIAL AND IONIC CONTENT IN PREGNANT AND NON-PREGNANT RAT MYOMETRIUM. J Physiol. 1965 Mar;177:263–287. doi: 10.1113/jphysiol.1965.sp007591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Casteels R., Kitamura K., Kuriyama H., Suzuki H. The membrane properties of the smooth muscle cells of the rabbit main pulmonary artery. J Physiol. 1977 Sep;271(1):41–61. doi: 10.1113/jphysiol.1977.sp011989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dimpfel W., Pierau F. K., Haider S. G. Electrophysiological studies on the effects of the neurotoxin apamin on cultured neurons. Adv Cytopharmacol. 1979;3:395–399. [PubMed] [Google Scholar]
  11. Flower R. J. Drugs which inhibit prostaglandin biosynthesis. Pharmacol Rev. 1974 Mar;26(1):33–67. [PubMed] [Google Scholar]
  12. Hara Y., Kitamura K., Kuriyama H. Actions of 4-aminopyridine on vascular smooth muscle tissues of the guinea-pig. Br J Pharmacol. 1980 Jan;68(1):99–106. doi: 10.1111/j.1476-5381.1980.tb10704.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kamikawa Y., Serizawa K., Shimo Y. Some possibilities for prostaglandin mediation in the contractile response to ATP of the guinea-pig digestive tract. Eur J Pharmacol. 1977 Sep 15;45(2):199–203. doi: 10.1016/0014-2999(77)90090-5. [DOI] [PubMed] [Google Scholar]
  14. Kawarabayashi T., Osa T. Comparative investigations of alpha- and beta-effects on the longitudinal and circular muscles of the pregnant rat myometrium. Jpn J Physiol. 1976;26(4):403–416. doi: 10.2170/jjphysiol.26.403. [DOI] [PubMed] [Google Scholar]
  15. Kishikawa T. Alterations in the properties of the rat myometrium during gestation and post partum. Jpn J Physiol. 1981;31(4):515–536. doi: 10.2170/jjphysiol.31.515. [DOI] [PubMed] [Google Scholar]
  16. Kroeger E. A., Marshall J. M. Beta-adrenergic effects on rat myometrium: mechanisms of membrane hyperpolarization. Am J Physiol. 1973 Dec;225(6):1339–1345. doi: 10.1152/ajplegacy.1973.225.6.1339. [DOI] [PubMed] [Google Scholar]
  17. Kuriyama H., Suzuki H. Changes in electrical properties of rat myometrium during gestation and following hormonal treatments. J Physiol. 1976 Sep;260(2):315–333. doi: 10.1113/jphysiol.1976.sp011517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kuriyama H., Suzuki H. Effects of prostaglandin E2 and oxytocin on the electrical activity of hormone-treated and pregnant rat myometria. J Physiol. 1976 Sep;260(2):335–349. doi: 10.1113/jphysiol.1976.sp011518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Magaribuchi T., Osa T. Effects of catecholamines on electrical and mechanical activities of the pregnant mouse myometrium. Jpn J Physiol. 1971 Dec;21(6):627–643. doi: 10.2170/jjphysiol.21.627. [DOI] [PubMed] [Google Scholar]
  20. Narahashi T. Chemicals as tools in the study of excitable membranes. Physiol Rev. 1974 Oct;54(4):813–889. doi: 10.1152/physrev.1974.54.4.813. [DOI] [PubMed] [Google Scholar]
  21. Needleman P., Minkes M. S., Douglas J. R., Jr Stimulation of prostaglandin biosynthesis by adenine nucleotides. Profile of prostaglandin release by perfused organs. Circ Res. 1974 Apr;34(4):455–460. doi: 10.1161/01.res.34.4.455. [DOI] [PubMed] [Google Scholar]
  22. Osa T. Effect of removing the external sodium on the electrical and mechanical activities of the pregnant mouse myometrium. Jpn J Physiol. 1971 Dec;21(6):607–625. doi: 10.2170/jjphysiol.21.607. [DOI] [PubMed] [Google Scholar]
  23. Osa T., Suzuki H., Katase T., Kuriyama H. Excitatory action of synthetic prostaglandin E2 on the electrical activity of pregnant mouse myometrium in relation to temperature changes and external sodium and calcium concentrations. Jpn J Physiol. 1974 Apr;24(2):233–248. doi: 10.2170/jjphysiol.24.233. [DOI] [PubMed] [Google Scholar]
  24. Suzuki H., Kuriyama H. Comparison between prostaglandin E2 and oxytocin actions on pregnant mouse myometrium. Jpn J Physiol. 1975;25(3):345–356. doi: 10.2170/jjphysiol.25.345. [DOI] [PubMed] [Google Scholar]
  25. Suzuki H., Kuriyama H. Effects of prostaglandin E2 on the electrical property of the pregnant mouse myometrium. Jpn J Physiol. 1975;25(2):201–215. doi: 10.2170/jjphysiol.25.201. [DOI] [PubMed] [Google Scholar]
  26. Tomita T., Watanabe H. A comparison of the effects of adenosine triphosphate with noradrenaline and with the inhibitory potential of the guinea-pig taenia coli. J Physiol. 1973 May;231(1):167–177. doi: 10.1113/jphysiol.1973.sp010226. [DOI] [PMC free article] [PubMed] [Google Scholar]

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