Abstract
A DNA probe combined with a non-radioactive stain was used to detect inclusions of Chlamydia trachomatis in cell culture. Of 39 positive cultures detected by monoclonal antibodies in combination with direct immunofluorescence, 35 were positive by the DNA hybridisation method, the sensitivity being 89.7%. Staining with iodine showed a sensitivity of 87.2%, corresponding to 34 positive cultures. The specificity of DNA hybridisation method was 100%, as all 162 cultures that were negative by the immunofluorescence method were also negative when assessed by the DNA hybridisation method.
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Selected References
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- Ambrosino D. M., Landesman S. H., Gorham C. C., Siber G. R. Passive immunization against disease due to Haemophilus influenzae type b: concentrations of antibody to capsular polysaccharide in high-risk children. J Infect Dis. 1986 Jan;153(1):1–7. doi: 10.1093/infdis/153.1.1. [DOI] [PubMed] [Google Scholar]
- Chernesky M. A., Mahony J. B., Castriciano S., Mores M., Stewart I. O., Landis S. J., Seidelman W., Sargeant E. J., Leman C. Detection of Chlamydia trachomatis antigens by enzyme immunoassay and immunofluorescence in genital specimens from symptomatic and asymptomatic men and women. J Infect Dis. 1986 Jul;154(1):141–148. doi: 10.1093/infdis/154.1.141. [DOI] [PubMed] [Google Scholar]
- Hambling M. H., Kurtz J. B. Preliminary evaluation of an enzyme immunoassay test for the detection of Chlamydia trachomatis. Lancet. 1985 Jan 5;1(8419):53–53. doi: 10.1016/s0140-6736(85)91007-4. [DOI] [PubMed] [Google Scholar]
- Hyypiä T., Jalava A., Larsen S. H., Terho P., Hukkanen V. Detection of Chlamydia trachomatis in clinical specimens by nucleic acid spot hybridization. J Gen Microbiol. 1985 Apr;131(4):975–978. doi: 10.1099/00221287-131-4-975. [DOI] [PubMed] [Google Scholar]
- Nowinski R. C., Tam M. R., Goldstein L. C., Stong L., Kuo C. C., Corey L., Stamm W. E., Handsfield H. H., Knapp J. S., Holmes K. K. Monoclonal antibodies for diagnosis of infectious diseases in humans. Science. 1983 Feb 11;219(4585):637–644. doi: 10.1126/science.6297006. [DOI] [PubMed] [Google Scholar]
- Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C. C., Holmes K. K., Ditzenberger K., Krieger M., Nowinski R. C. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. doi: 10.1056/NEJM198405033101803. [DOI] [PubMed] [Google Scholar]