Skip to main content
Genitourinary Medicine logoLink to Genitourinary Medicine
. 1990 Apr;66(2):87–90. doi: 10.1136/sti.66.2.87

Antibiotic resistance, plasmid profile, auxotypes and serovars of Neisseria gonorrhoeae strains isolated in Sevilla (Spain).

J C Palomares 1, M C Lozano 1, E J Perea 1
PMCID: PMC1194467  PMID: 2111276

Abstract

The antibiotics resistance pattern, the plasmid profile, the auxotypes and serotypes of 116 Neisseria gonorrhoeae clinical isolates obtained in one year were examined. The incidence of penicillinase producing (PPNG) strains was 12% (14 strains). The most frequent plasmid pattern was the combination of 4.5, 2.6 and 24.5 MDa plasmids. The conjugative plasmid of 24.5 MDa showed a high prevalence (32% of the total strains), and almost all the PPNG strains harboured this plasmid. The strains with the 4.5 MDa plasmid belonged to the auxotypes Pro-, Zero and Pro-Hyx-Ura-, whereas that with the 3.2 MDa plasmid was of auxotype Pro-Hyx-His-. The serotypes Aedih/Arst (WI serogroup) and Bak/Bropt, Back/Bropyt and Bak/Bropyt (WII/III serogroup) were predominant.

Full text

PDF
87

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ansink-Schipper M. C., Bygdeman S. M., van Klingeren B., Sandström E. G. Serovars, auxotypes, and plasmid profiles of PPNG strains with Asian type plasmid isolated in Amsterdam. Genitourin Med. 1988 Jun;64(3):152–155. doi: 10.1136/sti.64.3.152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ansink-Schipper M. C., Huikeshoven M. H., Woudstra R. K., van Klingeren B., de Koning G. A., Tio D., Schoonhoven F. J., Coutinho R. A. Epidemiology of PPNG infections in Amsterdam: analysis by auxanographic typing and plasmid characterisation. Br J Vener Dis. 1984 Feb;60(1):23–28. doi: 10.1136/sti.60.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ansink-Schipper M. C., van Klingeren B., Huikeshoven M. H., Woudstra R. K., Dessens-Kroon M., van Wijngaarden L. J. Epidemiology of PPNG infections in the Netherlands: analysis by auxanographic typing and plasmid identification. Br J Vener Dis. 1984 Jun;60(3):141–146. doi: 10.1136/sti.60.3.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bae B. H., Ledesma G., Korzis J. Analysis of Neisseria gonorrhoeae for in situ beta-lactamase production by reagent-impregnated filter paper replica methods. J Clin Microbiol. 1983 Mar;17(3):545–547. doi: 10.1128/jcm.17.3.545-547.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eckhardt T. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid. 1978 Sep;1(4):584–588. doi: 10.1016/0147-619x(78)90016-1. [DOI] [PubMed] [Google Scholar]
  6. Hall W. H., Schierl E. A., Maccani J. E. Comparative susceptibility of penicillinase-positive and -negative Neisseria gonorrhoeae to 30 antibiotics. Antimicrob Agents Chemother. 1979 Apr;15(4):562–567. doi: 10.1128/aac.15.4.562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Knapp J. S., Zenilman J. M., Biddle J. W., Perkins G. H., DeWitt W. E., Thomas M. L., Johnson S. R., Morse S. A. Frequency and distribution in the United States of strains of Neisseria gonorrhoeae with plasmid-mediated, high-level resistance to tetracycline. J Infect Dis. 1987 Apr;155(4):819–822. doi: 10.1093/infdis/155.4.819. [DOI] [PubMed] [Google Scholar]
  8. Korting H. C., Neubert U. Susceptibility of Neisseria gonorrhoeae to ceftizoxime in vitro and in vivo. Chemotherapy. 1984;30(5):322–327. doi: 10.1159/000238287. [DOI] [PubMed] [Google Scholar]
  9. Mavrommati L., Tzelepi E., Sima A., Tzanakaki G., Kokla A., Flemetakis A. Antibiotic resistance, plasmid content and auxotypes of Neisseria gonorrhoeae in Greece. J Antimicrob Chemother. 1988 May;21(5):571–579. doi: 10.1093/jac/21.5.571. [DOI] [PubMed] [Google Scholar]
  10. Mee B. J., Turbett G., Rich G. The epidemiology of Neisseria gonorrhoeae isolates from Western Australia based on antibiotic resistance and plasmid profiles. J Antimicrob Chemother. 1988 Jan;21(1):117–124. doi: 10.1093/jac/21.1.117. [DOI] [PubMed] [Google Scholar]
  11. Miller M. A., Anderson P., Parker J. W., Rohrer H. H. Inhibition of Neisseria gonorrhoeae isolates by Martin-Lewis medium. Epidemiology, susceptibility profile, and plasma analysis. Br J Vener Dis. 1982 Apr;58(2):96–100. doi: 10.1136/sti.58.2.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Roberts M., Piot P., Falkow S. The ecology of gonococcal plasmids. J Gen Microbiol. 1979 Oct;114(2):491–494. doi: 10.1099/00221287-114-2-491. [DOI] [PubMed] [Google Scholar]
  13. Zenilman J. M., Nims L. J., Menegus M. A., Nolte F., Knapp J. S. Spectinomycin-resistant gonococcal infections in the United States, 1985-1986. J Infect Dis. 1987 Dec;156(6):1002–1004. doi: 10.1093/infdis/156.6.1002. [DOI] [PubMed] [Google Scholar]

Articles from Genitourinary Medicine are provided here courtesy of BMJ Publishing Group

RESOURCES