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. 1983 Aug;341:641–654. doi: 10.1113/jphysiol.1983.sp014830

Vasoactive intestinal polypeptide: a putative transmitter in the canine gastric muscularis mucosa.

F Angel, V L Go, P F Schmalz, J H Szurszewski
PMCID: PMC1195355  PMID: 6194289

Abstract

The nature of the inhibitory transmitter in the canine gastric muscularis mucosae was studied in vitro using superfusion techniques. The inhibitory effect of nerve stimulation (10 V, 200 mus, 10 Hz) was not altered by adrenergic, cholinergic or serotonergic antagonists. Adenosine triphosphate had no effect on spontaneous mechanical activity. Nucleotide pyrophosphatase and apamin had no effect on the response to nerve stimulation. Alpha-chymotrypsin abolished the inhibitory effect of nerve stimulation. Radioimmunoassay of the muscle indicated the presence of gastrin/cholecystokinin-substance P- and vasoactive intestinal polypeptide (VIP)-like immunoreactivity. Of the three peptides present, only VIP produced a concentration-dependent relaxation. A substance with VIP-like immunoreactivity was released during nerve-induced relaxation of the muscle, and its release was blocked by tetrodotoxin and calcium-depleted solution. The inhibitory effect of nerve stimulation was abolished by VIP antiserum. These data strongly support the hypothesis that VIP or a closely related peptide is an inhibitory neurotransmitter in the canine gastric muscularis mucosae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angel F., Schmalz P. F., Morgan K. G., Go V. L., Szurszewski J. H. Innervation of the muscularis mucosa in the canine stomach and colon. Scand J Gastroenterol Suppl. 1982;71:71–75. [PubMed] [Google Scholar]
  2. Bitar K. N., Makhlouf G. M. Relaxation of isolated gastric smooth muscle cells by vasoactive intestinal peptide. Science. 1982 Apr 30;216(4545):531–533. doi: 10.1126/science.6176025. [DOI] [PubMed] [Google Scholar]
  3. Burnstock G. Review lecture. Neurotransmitters and trophic factors in the autonomic nervous system. J Physiol. 1981;313:1–35. doi: 10.1113/jphysiol.1981.sp013648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burnstock G. Studies of autonomic nerves in the gut-past, present and future. Scand J Gastroenterol Suppl. 1982;71:135–138. [PubMed] [Google Scholar]
  5. Costa M., Furness J. B., Buffa R., Said S. I. Distribution of enteric nerve cell bodies and axons showing immunoreactivity for vasoactive intestinal polypeptide in the guinea-pig intestine. Neuroscience. 1980;5(3):587–596. doi: 10.1016/0306-4522(80)90056-1. [DOI] [PubMed] [Google Scholar]
  6. Edin R., Lundberg J. M., Ahlman H., Dahlström A., Fahrenkrug J., Hökfelt T., Kewenter J. On the VIP-ergic innervation of the feline pylorus. Acta Physiol Scand. 1979 Oct;107(2):185–187. doi: 10.1111/j.1748-1716.1979.tb06461.x. [DOI] [PubMed] [Google Scholar]
  7. Furness J. B., Costa M., Murphy R., Beardsley A. M., Oliver J. R., Llewellyn-Smith I. J., Eskay R. L., Shulkes A. A., Moody T. W., Meyer D. K. Detection and characterisation of neurotransmitters, particularly peptides, in the gastrointestinal tract. Scand J Gastroenterol Suppl. 1982;71:61–70. [PubMed] [Google Scholar]
  8. Jessen K. R., Polak J. M., Van Noorden S., Bloom S. R., Burnstock G. Peptide-containing neurones connect the two ganglionated plexuses of the enteric nervous system. Nature. 1980 Jan 24;283(5745):391–393. doi: 10.1038/283391a0. [DOI] [PubMed] [Google Scholar]
  9. Mackenzie I., Burnstock G. Evidence against vasoactive intestinal polypeptide being the non-adrenergic, non-cholinergic inhibitory transmitter released from nerves supplying the smooth muscle of the guinea-pig taenia coli. Eur J Pharmacol. 1980 Oct 17;67(2-3):255–264. doi: 10.1016/0014-2999(80)90506-3. [DOI] [PubMed] [Google Scholar]
  10. Reinecke M., Schlüter P., Yanaihara N., Forssmann W. G. VIP immunoreactivity in enteric nerves and endocrine cells of the vertebrate gut. Peptides. 1981;2 (Suppl 2):149–156. doi: 10.1016/0196-9781(81)90025-5. [DOI] [PubMed] [Google Scholar]
  11. Saffrey M. J., Polak J. M., Burnstock G. Distribution of vasoactive intestinal polypeptide-, substance P-, enkephalin and neurotensin-like immunoreactive nerves in the chicken gut during development. Neuroscience. 1982 Jan;7(1):279–293. doi: 10.1016/0306-4522(82)90166-x. [DOI] [PubMed] [Google Scholar]
  12. Satchell D. G. Nucleotide pyrophosphatase antagonizes responses to adenosine 5'-triphosphate and non-adrenergic, non-cholinergic inhibitory nerve stimulation in the guinea-pig isolated taenia coli. Br J Pharmacol. 1981 Oct;74(2):319–321. doi: 10.1111/j.1476-5381.1981.tb09973.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schaffalitzky de Muckadell O. B., Fahrenkrug J., Holst J. J. Release of vasoactive intestinal polypeptide (VIP) by electric stimulation of the vagal nerves. Gastroenterology. 1977 Feb;72(2):373–375. [PubMed] [Google Scholar]
  14. Schultzberg M., Hökfelt T., Nilsson G., Terenius L., Rehfeld J. F., Brown M., Elde R., Goldstein M., Said S. Distribution of peptide- and catecholamine-containing neurons in the gastro-intestinal tract of rat and guinea-pig: immunohistochemical studies with antisera to substance P, vasoactive intestinal polypeptide, enkephalins, somatostatin, gastrin/cholecystokinin, neurotensin and dopamine beta-hydroxylase. Neuroscience. 1980;5(4):689–744. doi: 10.1016/0306-4522(80)90166-9. [DOI] [PubMed] [Google Scholar]
  15. Service F. J., Nelson R. L., Rubenstein A. H., Go V. L. Direct effect of insulin on secretion of insulin, glucagon, gastric inhibitory polypeptide, and gastrin during maintenance of normoglycemia. J Clin Endocrinol Metab. 1978 Sep;47(3):488–493. doi: 10.1210/jcem-47-3-488. [DOI] [PubMed] [Google Scholar]
  16. Yaksh T. L., Abay E. O., 2nd, Go V. L. Studies on the location and release of cholecystokinin and vasoactive intestinal peptide in rat and cat spinal cord. Brain Res. 1982 Jun 24;242(2):279–290. doi: 10.1016/0006-8993(82)90311-0. [DOI] [PubMed] [Google Scholar]
  17. Yaksh T. L., Schmauss C., Micevych P. E., Abay E. O., Go V. L. Pharmacological studies on the application, disposition, and release of neurotensin in the spinal cord. Ann N Y Acad Sci. 1982;400:228–243. doi: 10.1111/j.1749-6632.1982.tb31572.x. [DOI] [PubMed] [Google Scholar]

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