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. 1982;333:69–79. doi: 10.1113/jphysiol.1982.sp014439

Catecholamine synthesis in rabbit carotid body in vitro

S Fidone 1, C Gonzalez 1
PMCID: PMC1197234  PMID: 6820664

Abstract

1. Catecholamine synthesis in rabbit carotid body was studied in vitro using [3H]DOPA and [3H]tyrosine as precursors. The effects of sympathectomy and transection of the carotid sinus nerve on [3H]dopamine ([3H]DA) and [3H]noradrenaline ([3H]NA) synthesis were investigated in chronically denervated carotid bodies.

2. When [3H]DOPA was used as precursor, the synthesis of [3H]DA was linear for more than 6 hr. The carotid body synthesized larger amounts of [3H]catecholamines than when [3H]tyrosine was used as precursor, but most of this excess was liberated into the incubation media. Using 10 μM-[3H]DOPA as precursor, the synthesis rates were 6·76 and 1·51 n-mole/g per hr for [3H]DA and [3H]NA, respectively; with 40 μM-[3H]DOPA, these values increased to 19·22 and 3·23 n-mole/g per hr, respectively.

3. The relationship between [3H]DOPA concentration and [3H]DA synthesis was linear throughout the range 5-40 μM-[3H]DOPA.

4. Sympathectomy reduced the synthesis of [3H]NA by 90% and [3H]DA by 37% when [3H]DOPA was used as precursor.

5. When [3H]tyrosine (40 μM) was used as precursor, synthesis of [3H]catecholamines was linear for at least 4 hr, with rates of 12·10 and 0·85 n-mole/g per hr for [3H]DA and [3H]NA, respectively.

6. [3H]DA and [3H]NA synthesis from [3H]tyrosine exhibited the characteristics of saturable processes, with Km values of 16·8 and 17·6 μM, respectively.

7. 6-methyltetrahydropterine (6-MPH4, 100 μM), a synthetic analogue of the natural co-factor for tyrosine hydroxylase, increased [3H]DA and [3H]NA synthesis from [3 H]tyrosine in both the carotid body and superior cervical ganglion, with the greatest effect seen in the carotid body.

8. When [3H]tyrosine was used as precursor, sympathectomy of the carotid body reduced [3H]NA synthesis by 80%, but did not alter [3H]DA or [3H]tyrosine levels in the tissue. Transection of the carotid sinus nerve had no effect on [3H]catecholamine synthesis in the carotid body.

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Selected References

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