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. 1982 Oct;331:547–555. doi: 10.1113/jphysiol.1982.sp014390

Role of cyclic adenosine monophosphate in amylase release from dissociated rat pancreatic acini

Manjit Singh 1,2
PMCID: PMC1197767  PMID: 6185668

Abstract

1. The effect of octapeptide of cholecystokinin-pancreozymin (CCK8), bethanechol, cholera toxin, glucagon and vasoactive intestinal polypeptide (VIP) on amylase secretion and lactic dehydrogenase (LDH) release from isolated rat pancreatic acini was studied.

2. In isolated rat pancreatic acini, in the absence of theophylline in the medium, amylase secretion was increased by 65-78% with 10-7 and 10-6 M-cholera toxin. In the presence of theophylline, amylase secretion was increased by 43-56% with 10-7 and 10-6 M-cholera toxin following a 90 min incubation. No effect was observed in the presence of theophylline at 30 and 60 min. The effect of cholera toxin was potentiated by CCK8 at 60 and 90 min.

3. In the absence of theophylline in the medium, amylase secretion was increased by 81-118% with 10-5 and 10-4 M-glucagon and 86% with 10-6 M-VIP at 60 min. In the presence of theophylline in the medium, amylase secretion was increased by 53-246% with 10-9 to 10-6 M-glucagon and 111-158% with 10-7 and 10-6 M-VIP respectively. The effect of glucagon and VIP was potentiated by CCK8.

4. Potentiation of the rate of amylase release due to glucagon (10-5 M) and VIP (10-6 M) occurred during the first 15 min of incubation.

5. Release of LDH was not increased by any of these agents.

6. It is concluded that cyclic AMP rise (due to cholera toxin, glucagon and VIP effect) increased amylase secretion from rat pancreatic acinar cells. This effect is less marked than in the guinea-pig pancreas and is potentiated by agents mobilizing cellular Ca2+ (CCK8 and bethanechol).

7. These data indicate species-specific variation in the action of cyclic AMP in the pancreas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauduin H., Stock C., Launay J. F., Vincent D., Potvliege P., Grenier J. F. On the secretagogue effect of dibutyryl cyclic AMP in the rat exocrine pancreas. Pflugers Arch. 1977 Nov 25;372(1):69–76. doi: 10.1007/BF00582208. [DOI] [PubMed] [Google Scholar]
  2. Bommelaer G., Rozental G., Bernier C., Vaysse N., Ribet A. Action of secretagogues on amylase release from dog pancreatic acini. Digestion. 1981;21(5):248–254. doi: 10.1159/000198570. [DOI] [PubMed] [Google Scholar]
  3. Case R. M. Synthesis, intracellular transport and discharge of exportable proteins in the pancreatic acinar cell and other cells. Biol Rev Camb Philos Soc. 1978 May;53(2):211–354. doi: 10.1111/j.1469-185x.1978.tb01437.x. [DOI] [PubMed] [Google Scholar]
  4. Deschodt-Lanckman M., Robberecht P., De Neef P., Labrie F., Christophe J. In vitro interactions of gastrointestinal hormones on cyclic adenosine 3':5'-monophosphate levels and amylase output in the rat pancreas. Gastroenterology. 1975 Feb;68(2):318–325. [PubMed] [Google Scholar]
  5. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  6. Gardner J. D., Conlon T. P., Adams T. D. Cyclic AMP in pancreatic acinar cells: effects of gastrointestinal hormones. Gastroenterology. 1976 Jan;70(1):29–35. [PubMed] [Google Scholar]
  7. Gardner J. D., Jackson M. J. Regulation of amylase release from dispersed pancreatic acinar cells. J Physiol. 1977 Sep;270(2):439–454. doi: 10.1113/jphysiol.1977.sp011961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gardner J. D., Rottman A. J. Action of cholera toxin on dispersed acini from guinea pig pancreas. Biochim Biophys Acta. 1979 Jun 12;585(2):250–265. doi: 10.1016/0304-4165(79)90025-4. [DOI] [PubMed] [Google Scholar]
  9. Heisler S., Grondin G., Forget G. The effect of various secretagogues on accumulation of cyclic AMP and secretion of alpha-amylase from rat exocrine pancreas. Life Sci. 1974 Feb 16;14(4):631–639. doi: 10.1016/0024-3205(74)90398-1. [DOI] [PubMed] [Google Scholar]
  10. Kempen H. J., de Pont J. J., Bonting S. L. Rat pancreas adenylate cyclase V. Its presence in isolated rat pancreatic acinar cells. Biochim Biophys Acta. 1977 Feb 28;496(2):521–531. doi: 10.1016/0304-4165(77)90333-6. [DOI] [PubMed] [Google Scholar]
  11. Kempen H. J., de Pont J. J., Bonting S. L. Rat pancreas adenylate cyclase. III. Its role in pancreatic secretion assessed by means of choleara toxin. Biochim Biophys Acta. 1975 Jun 12;392(2):276–287. doi: 10.1016/0304-4165(75)90009-4. [DOI] [PubMed] [Google Scholar]
  12. Mekalanos J. J., Collier R. J., Romig W. R. Purification of cholera toxin and its subunits: new methods of preparation and the use of hypertoxinogenic mutants. Infect Immun. 1978 May;20(2):552–558. doi: 10.1128/iai.20.2.552-558.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morisset J. A., Webster P. D. In vitro and in vivo effects of pancreozymin, urecholine, and cyclic AMP on rat pancreas. Am J Physiol. 1971 Jan;220(1):202–208. doi: 10.1152/ajplegacy.1971.220.1.202. [DOI] [PubMed] [Google Scholar]
  14. Robberecht P., Conlon T. P., Gardner J. D. Interaction of porcine vasoactive intestinal peptide with dispersed pancreatic acinar cells from the guinea pig. Structural requirements for effects of vasoactive intestinal peptide and secretin on cellular adenosine 3':5'-monophosphate. J Biol Chem. 1976 Aug 10;251(15):4635–4639. [PubMed] [Google Scholar]
  15. Robberecht P., Deschodt-Lanckman M., De Neef P., Borgeat P., Christophe J. In vivo effects of pancreozymin, secretin, vasoactive intestinal polypeptide and pilocarpine on the levels of cyclic AMP and cyclic GMP in the rat pancreas. FEBS Lett. 1974 Jul 15;43(2):139–143. doi: 10.1016/0014-5793(74)80986-5. [DOI] [PubMed] [Google Scholar]
  16. Robberecht P., Deschodt-Lanckman M., Lammens M., De Neff P., Christophe J. "In vitro" effects of secretin and vasoactive intestinal polypeptide on hydrolase secretion and cylic AMP levels in the pancreas of five animal species. A comparison with caerulein. Gastroenterol Clin Biol. 1977 Jun-Jul;1(6-7):519–525. [PubMed] [Google Scholar]
  17. Schafer D. E., Lust W. D., Sircar B., Goldberg N. D. Elevated concentration of adenosine 3':5'-cyclic monophosphate in intestinal mucosa after treatment with cholera toxin. Proc Natl Acad Sci U S A. 1970 Oct;67(2):851–856. doi: 10.1073/pnas.67.2.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Singh M. Amylase release from dissociated mouse pancreatic acinar cells stimulated by glucagon: effect of membrane stabilizers. J Physiol. 1980 Dec;309:81–91. doi: 10.1113/jphysiol.1980.sp013495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Singh M. Calcium and cyclic nucleotide interaction in secretion of amylase from rat pancreas in vitro. J Physiol. 1979 Nov;296:159–176. doi: 10.1113/jphysiol.1979.sp012997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Singh M. Effect of glucagon on digestive enzyme synthesis, transport and secretion in mouse pancreatic acinar cells. J Physiol. 1980 Sep;306:307–322. doi: 10.1113/jphysiol.1980.sp013398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Singh M. Effect of vitamin B6 deficiency on pancreatic acpinar cell function. Life Sci. 1980 Mar 3;26(9):715–724. doi: 10.1016/0024-3205(80)90261-1. [DOI] [PubMed] [Google Scholar]
  22. Smith P. A., Case R. M. Effects of cholera toxin on cyclic adenosine 3',5'-monophosphate concentration and secretory processes in the exocrine pancreas. Biochim Biophys Acta. 1975 Aug 13;399(2):277–290. doi: 10.1016/0304-4165(75)90258-5. [DOI] [PubMed] [Google Scholar]
  23. Williams J. A., Korc M., Dormer R. L. Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. Am J Physiol. 1978 Nov;235(5):517–524. doi: 10.1152/ajpendo.1978.235.5.E517. [DOI] [PubMed] [Google Scholar]

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