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. 1967 Oct;105(1):35–38. doi: 10.1042/bj1050035

Purification of an acid α-glucosidase by dextran-gel filtration

F Auricchio 1, C B Bruni 1
PMCID: PMC1198270  PMID: 6056633

Abstract

1. The behaviour of rat liver α-glucosidases on dextran gel (Sephadex G-100) columns was studied. A `retardation' of an acid α-glucosidase activity was observed. This activity was identified as lysosome α-(1→4)-glucosidase. A single gel-filtration step resulted in a 700-fold purification of the enzyme. The same technique was also used to purify the acid α-glucosidase of human kidney. 2. The acid α-glucosidases of both tissues show very similar pH optima when tested with maltose or glycogen as substrate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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