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. 1967 Oct;105(1):251–259. doi: 10.1042/bj1050251

Effect of some polycyclic aromatic hydrocarbons on protein synthesis in vitro

J Hradec 1
PMCID: PMC1198296  PMID: 4168142

Abstract

1. The effect of the strongly carcinogenic polycyclic aromatic hydrocarbons benzo[a]pyrene, 3-methylcholanthrene and dibenz[a,h]anthracene and of the non-carcinogenic anthracene, pyrene and phenanthrene on protein synthesis was studied in vitro with subcellular systems from rat liver. 2. Both types of hydrocarbons affect amino acid activation and inhibit transfer of labelled amino acids from transfer RNA to ribosomes. 3. Only the carcinogenic compounds stimulate the incorporation of labelled algal-protein hydrolysate and of some individual amino acids into transfer RNA. The most active dose was 10mμg. under the conditions used. This effect is abolished by preincubation of pH5 enzymes with the carcinogens before the addition of radioactive amino acids. 4. The carcinogens stimulate the incorporation of some amino acids into ribosomal protein whereas the non-carcinogenic compounds have no such effects. 5. Polynucleotide-dependent stimulation of protein synthesis is greatly enhanced in the presence of the carcinogenic hydrocarbons when either free amino acids or transfer RNA charged with labelled amino acids are used. The non-carcinogenic compounds induce a partial inhibition of this process. 6. It is concluded, in agreement with other authors, that carcinogens may increase the number of active incorporation sites on both transfer and ribosomal RNA. Possible mechanisms of such an effect are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARRHENIUS E., HULTIN T. Effects of carcinogenic amines on amino acid incorporation by liver systems. I. Secondary increase in microsomal activity after aminofluorene treatment. Cancer Res. 1962 Aug;22:823–834. [PubMed] [Google Scholar]
  2. Bresnick E., Brand R., Knight J. A. Ribonucleic acid biosynthesis in methylcholanthrene-treated rats. Biochim Biophys Acta. 1966 Feb 21;114(2):227–233. doi: 10.1016/0005-2787(66)90304-2. [DOI] [PubMed] [Google Scholar]
  3. Bresnick E., Knight J. A., Madix J. C. Polyribosomal patterns in rat liver after the administration of 3-methylcholanthrene. Biochim Biophys Acta. 1966 Jun 22;119(3):624–626. doi: 10.1016/0005-2787(66)90138-9. [DOI] [PubMed] [Google Scholar]
  4. CHANG M., BOND T. J. EFFECT OF CERTAIN POLYCYCLIC HYDROCARBONS ON THE INCORPORATION OF AMINO-ACIDS INTO SOLUBLE RIBONUCLEIC ACIDS. Nature. 1964 Feb 8;201:623–623. doi: 10.1038/201623a0. [DOI] [PubMed] [Google Scholar]
  5. GELBOIN H. V., BLACKBURN N. R. THE STIMULATORY EFFECT OF 3-METHYLCHOLANTHRENE ON MICROSOMAL AMINO ACID INCORPORATION AND BENZPYRENE HYDROXYLASE ACTIVITY AND ITS INHIBITION BY ACTINOMYCIN D. Biochim Biophys Acta. 1963 Aug 20;72:657–660. [PubMed] [Google Scholar]
  6. GELBOIN H. V., SOKOLOFF L. Effects of 3-methylcholanthrene and phenobarbital on amino acid incorporation into protein. Science. 1961 Sep 1;134(3479):611–612. doi: 10.1126/science.134.3479.611. [DOI] [PubMed] [Google Scholar]
  7. GELBOIN H. V. STUDIES ON THE MECHANISM OF METHYLCHOLANTHRENE INDUCTION OF ENZYME ACTIVITIES. II. STIMULATION OF MICROSOMAL AND RIBOSOMAL AMINO ACID INCORPORATION: THE EFFECTS OF POLYURIDYLIC ACID AND ACTINOMYCIN-D. Biochim Biophys Acta. 1964 Sep 11;91:130–144. [PubMed] [Google Scholar]
  8. HAWTREY A. O., SCHIRREN V. The effect of the carcinogen 3'-methyl-4-dimethylaminoazobenzene on protein synthesis by rat-liver microsomes. Biochim Biophys Acta. 1962 May 14;55:785–786. doi: 10.1016/0006-3002(62)90860-0. [DOI] [PubMed] [Google Scholar]
  9. HOAGLAND M. B., STEPHENSON M. L., SCOTT J. F., HECHT L. I., ZAMECNIK P. C. A soluble ribonucleic acid intermediate in protein synthesis. J Biol Chem. 1958 Mar;231(1):241–257. [PubMed] [Google Scholar]
  10. HRADEC J. Effect of carcinogens and related compounds on the growth of Ehrlich ascites carcinoma and its possible mechanism. Br J Cancer. 1959 Jun;13:336–347. doi: 10.1038/bjc.1959.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HRADEC J. Effect of carcinolipin on protein synthesis in cell-free systems. Biochim Biophys Acta. 1961 Feb 12;47:149–157. doi: 10.1016/0006-3002(61)90840-x. [DOI] [PubMed] [Google Scholar]
  12. HULTIN T., ARRHENIUS E. EFFECTS OF CARCINOGENIC AMINES ON AMINO ACID INCORPORATION BY LIVER SYSTEMS. 3. INHIBITION BY AMINOFLUORENE TREATMENT AND ITS DEPENDENCE ON VITAMIN E. Cancer Res. 1965 Feb;25:124–131. [PubMed] [Google Scholar]
  13. Jondorf W. R., Spector A., Chaiken S. J. The effect of two carcinostatic agents on the chemically induced stimulation of amino acid incorporation in a mammalian system. Biochem Biophys Res Commun. 1965 Sep 22;20(6):787–792. doi: 10.1016/0006-291x(65)90087-2. [DOI] [PubMed] [Google Scholar]
  14. KELLER E. B., LITTLEFIELD J. W. Incorporation of C14-amino acids into ribonucleoprotein particles from the Ehrlich mouse ascites tumor. J Biol Chem. 1957 Jan;224(1):13–30. [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Libby P. R., Dao T. L. Rat Mammary Gland RNA: Incorporation of C14-Formate and Effect of Hormones and 7,12-Dimethylbenz[alanthracene. Science. 1966 Jul 15;153(3733):303–305. doi: 10.1126/science.153.3733.303. [DOI] [PubMed] [Google Scholar]
  17. MOLDAVE K. The labeling in vitro of intermediates in amino acid incorporation. J Biol Chem. 1960 Aug;235:2365–2369. [PubMed] [Google Scholar]
  18. WEBSTER L. T., Jr, DAVIE E. W. Purification and properties of serine-activating enzyme from beef pancreas. J Biol Chem. 1961 Feb;236:479–484. [PubMed] [Google Scholar]

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