Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1967 Nov;105(2):605–609. doi: 10.1042/bj1050605

Studies on the biosynthesis of protein and lipid components of rat liver mitochondria

C B Taylor 1, E Bailey 1, W Bartley 1
PMCID: PMC1198351  PMID: 5584003

Abstract

1. Male rats were injected intraperitoneally with l-[35S]methionine, [32P]-phosphate and [2-14C]acetate. The animals were killed at various times up to 72hr. after injection, and liver mitochondria were prepared and fractionated into soluble protein, insoluble protein and lipid for assay of the radioactivity of each fraction. 2. The maximal specific radioactivity of total mitochondrial phospholipid with respect to both 32P and 14C was attained after approx. 6hr. 3. 32P was incorporated most rapidly into phosphatidylethanolamine, maximal incorporation being attained after approx. 6hr.; maximal incorporation into lecithin occurred after 6–12hr. The specific radioactivity of cardiolipin was still slowly increasing at the end of the experiment (72hr.). 4. There were no major differences between the rates of incorporation of 14C into the lecithin, phosphatidylethanolamine and cardiolipin fractions of mitochondrial phospholipid, maximal incorporation in each case occurring after approx. 6hr. 5. Maximal incorporation of 35S into both soluble and insoluble protein fractions was attained less than 12hr. after injection, the maximal specific radioactivity of soluble protein being higher than that of insoluble protein.

Full text

PDF
605

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey E., Taylor C. B., Bartley W. Turnover of mitochondrial components of normal and essential fatty acid-deficient rats. Biochem J. 1967 Sep;104(3):1026–1032. doi: 10.1042/bj1041026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beattie D. S., Basford R. E., Koritz S. B. Studies on the biosynthesis of mitochondrial protein components. Biochemistry. 1966 Mar;5(3):926–930. doi: 10.1021/bi00867a018. [DOI] [PubMed] [Google Scholar]
  3. FLETCHER M. J., SANADI D. R. Turnover of rat-liver mitochondria. Biochim Biophys Acta. 1961 Aug 5;51:356–360. doi: 10.1016/0006-3002(61)90177-9. [DOI] [PubMed] [Google Scholar]
  4. Gurr M. I., Prottey C., Hawthorne J. N. The phospholipids of liver-cell fractions. II. Incorporation of [32P]orthophosphate in vivo in normal and regenerating rat liver. Biochim Biophys Acta. 1965 Oct 4;106(2):357–370. [PubMed] [Google Scholar]
  5. Haldar D., Freeman K., Work T. S. Biogenesis ommitochondria. Nature. 1966 Jul 2;211(5044):9–12. doi: 10.1038/211009a0. [DOI] [PubMed] [Google Scholar]
  6. King E. J. The colorimetric determination of phosphorus. Biochem J. 1932;26(2):292–297. doi: 10.1042/bj0260292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. PASCAUD M. LES PHOSPHOLIPIDES DE LA CELLULE H'EPATIQUE. INTERPR'ERATION FONCTIONNELLE DE LEUR RENOUVELLEMENT. II. RENOUVELLEMENT DES ACIDES GRAS DES PHOSPHOGLYCERIDES. Biochim Biophys Acta. 1964 Oct 2;84:528–537. [PubMed] [Google Scholar]
  8. WILGRAM G. F., KENNEDY E. P. INTRACELLULAR DISTRIBUTION OF SOME ENZYMES CATALYZING REACTIONS IN THE BIOSYNTHESIS OF COMPLEX LIPIDS. J Biol Chem. 1963 Aug;238:2615–2619. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES