Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1967 Dec;105(3):943–946. doi: 10.1042/bj1050943

The activity of uridine diphosphate glucose–d-fructose 6-phosphate 2-glucosyltransferase in leaves

J S Hawker 1,*
PMCID: PMC1198411  PMID: 16742569

Abstract

1. By using EDTA in reaction mixtures it was possible to determine the activity of sucrose phosphate synthetase in freshly prepared leaf extracts without the complications caused by sucrose phosphatase. 2. EDTA was found also to increase the activity of sucrose phosphate synthetase by as much as 100%. 3. High sucrose phosphate synthetase activities were found in leaf preparations in which sucrose phosphatase was inhibited by EDTA. By contrast with previous reports, the activities were sufficient to allow sucrose synthesis in leaves during photosynthesis to occur via sucrose phosphate. 4. Sugar-cane plants having different rates of photosynthesis also had different activities of sucrose phosphate synthetase in their leaves. 5. It is suggested that the activity of sucrose phosphate synthetase in leaves may play a role in the control of the rate of photosynthesis.

Full text

PDF
943

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIRD I. F., PORTER H. K., STOCKING C. R. INTRACELLULAR LOCALISATION OF ENZYMES ASSOCIATED WITH SUCROSE SYNTHESIS IN LEAVES. Biochim Biophys Acta. 1965 May 4;100:366–375. doi: 10.1016/0304-4165(65)90005-x. [DOI] [PubMed] [Google Scholar]
  2. Ghosh H. P., Preiss J. Adenosine diphosphate glucose pyrophosphorylase. A regulatory enzyme in the biosynthesis of starch in spinach leaf chloroplasts. J Biol Chem. 1966 Oct 10;241(19):4491–4504. [PubMed] [Google Scholar]
  3. Haq S., Hassid W. Z. Biosynthesis of sucrose phosphate with sugar cane leaf chloroplasts. Plant Physiol. 1965 Jul;40(4):591–594. doi: 10.1104/pp.40.4.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hatch M. D., Sacher J. A., Glasziou K. T. Sugar Accumulation Cycle in Sugar Cane. I. Studies on Enzymes of the Cycle. Plant Physiol. 1963 May;38(3):338–343. doi: 10.1104/pp.38.3.338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hatch M. D. Sugar accumulation by sugar-cane storage tissue: the role of sucrose phosphate. Biochem J. 1964 Dec;93(3):521–526. doi: 10.1042/bj0930521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hawker J. S., Hatch M. D. A specific sucrose phosphatase from plant tissues. Biochem J. 1966 Apr;99(1):102–107. doi: 10.1042/bj0990102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hawker J. S. Inhibition of sucrose phosphatase by sucrose. Biochem J. 1967 Feb;102(2):401–406. doi: 10.1042/bj1020401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LELOIR L. F., CARDINI C. E. The biosynthesis of sucrose phosphate. J Biol Chem. 1955 May;214(1):157–165. [PubMed] [Google Scholar]
  9. MENDICINO J. Sucrose phosphate synthesis in wheat germ and green leaves. J Biol Chem. 1960 Dec;235:3347–3352. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES