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. 1967 Dec;105(3):1019–1028. doi: 10.1042/bj1051019

The C-terminal sequences of the heavy chains of human immunoglobulin G myeloma proteins of differing isotopes and allotypes

J W Prahl 1,*
PMCID: PMC1198421  PMID: 16742526

Abstract

The sequences of the C-terminal octadecapeptides obtained by cyanogen bromide cleavage of the γ-chains of myeloma proteins of the four subclasses, and a urinary heavy-chain-disease protein, have been determined. Although the sequences were markedly homologous, unique replacements were identified that distinguished between the γ2b, γ2c and γ2d subclasses. The data are in accord with the postulated existence of four genetic loci or cistrons, these having arisen by the process of gene duplication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALLIEUX R. E., BERNIER G. M., TOMINAGA K., PUTNAM F. W. GAMMA GLOBULIN ANTIGENIC TYPES DEFINED BY HEAVY CHAIN DETERMINANTS. Science. 1964 Jul 10;145(3628):168–170. doi: 10.1126/science.145.3628.168. [DOI] [PubMed] [Google Scholar]
  2. BRADBURY J. H. The kinetics of hydrazinolysis of simple peptides in anhydrous hydrazine. Biochem J. 1958 Mar;68(3):475–482. doi: 10.1042/bj0680475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLELAND W. W. DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS. Biochemistry. 1964 Apr;3:480–482. doi: 10.1021/bi00892a002. [DOI] [PubMed] [Google Scholar]
  4. CRUMPTON M. J., WILKINSON J. M. AMINO ACID COMPOSITIONS OF HUMAN AND RABBIT GAMMA-GLOBULINS AND OF THE FRAGMENTS PRODUCED BY REDUCTION. Biochem J. 1963 Aug;88:228–234. doi: 10.1042/bj0880228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crumpton M. J., Wilkinson J. M. The immunological activity of some of the chymotryptic peptides of sperm-whale myoglobin. Biochem J. 1965 Mar;94(3):545–556. doi: 10.1042/bj0940545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Easley C. W. Combinations of specific color reactions useful in the peptide mapping technique. Biochim Biophys Acta. 1965 Sep 13;107(2):386–388. doi: 10.1016/0304-4165(65)90147-9. [DOI] [PubMed] [Google Scholar]
  7. FLEISCHMAN J. B., PAIN R. H., PORTER R. R. Reduction of gamma-globulins. Arch Biochem Biophys. 1962 Sep;Suppl 1:174–180. [PubMed] [Google Scholar]
  8. FRANKLIN E. C., KUNKEL H. G., MULLER-EBERHARD H. J. Two types of gamma-globulin differing in carbohydrate content. Proc Soc Exp Biol Med. 1956 Oct;93(1):146–150. doi: 10.3181/00379727-93-22690. [DOI] [PubMed] [Google Scholar]
  9. Frangione B., Franklin E. C., Fudenberg H. H., Koshland M. E. Structural studies of human gamma-G-myeloma proteins of different antigenic subgroups and genetic specificities. J Exp Med. 1966 Oct 1;124(4):715–732. doi: 10.1084/jem.124.4.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GREY H. M., KUNKEL H. G. H CHAIN SUBGROUPS OF MYELOMA PROTEINS AND NORMAL 7S GAMMA-GLOBULIN. J Exp Med. 1964 Aug 1;120:253–266. doi: 10.1084/jem.120.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GRUBB R. Agglutination of erythrocytes coated with incomplete anti-Rh by certain rheumatoid arthritic sera and some other sera; the existence of human serum groups. Acta Pathol Microbiol Scand. 1956;39(3):195–197. [PubMed] [Google Scholar]
  12. GRUBB R., LAURELL A. B. Hereditary serological human serum groups. Acta Pathol Microbiol Scand. 1956;39(6):390–398. doi: 10.1111/j.1699-0463.1956.tb05067.x. [DOI] [PubMed] [Google Scholar]
  13. GUNDLACH H. G., STEIN W. H., MOORE S. The nature of the amino acid residues involved in the inactivation of ribonuclease by iodoacetate. J Biol Chem. 1959 Jul;234(7):1754–1760. [PubMed] [Google Scholar]
  14. Gold E. R., Mandy W. J., Fudenberg H. H. Relation between Gm(f) and the structure of the gamma-globulin molecule. Nature. 1965 Sep 4;207(5001):1099–1101. doi: 10.1038/2071099a0. [DOI] [PubMed] [Google Scholar]
  15. HARBOE M., OSTERLAND C. K., KUNKEL H. G. Localization of two genetic factors to different areas of gamma-globulin molecules. Science. 1962 Jun 15;136(3520):979–980. doi: 10.1126/science.136.3520.979. [DOI] [PubMed] [Google Scholar]
  16. KONIGSBERG W., HILL R. J. The structure of human hemoglobin. III. The sequence of amino acids in the tryptic peptides of the alpha chain. J Biol Chem. 1962 Aug;237:2547–2561. [PubMed] [Google Scholar]
  17. KOSTKA V., CARPENTER F. H. INHIBITION OF CHYMOTRYPSIN ACTIVITY IN CRYSTALLINE TRYPSIN PREPARATIONS. J Biol Chem. 1964 Jun;239:1799–1803. [PubMed] [Google Scholar]
  18. KUNKEL H. G., ALLEN J. C., GREY H. M., MARTENSSON L., GRUBB R. A RELATIONSHIP BETWEEN THE H CHAIN GROUPS OF 7S GAMMA-GLOBULIN AND THE GM SYSTEM. Nature. 1964 Jul 25;203:413–414. doi: 10.1038/203413a0. [DOI] [PubMed] [Google Scholar]
  19. Kekwick R. A. The serum proteins in multiple myelomatosis. Biochem J. 1940 Sep;34(8-9):1248–1257. doi: 10.1042/bj0341248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kunkel H. G., Yount W. J., Litwin S. D. Genetically determined antigen of the Ne subgroup of gamma-globulin: detection by precipitin analysis. Science. 1966 Nov 25;154(3752):1041–1043. doi: 10.1126/science.154.3752.1041. [DOI] [PubMed] [Google Scholar]
  21. LAWLER S. D., COHEN S. DISTRIBUTION OF ALLOTYPIC SPECIFICITIES ON THE PEPTIDE CHAINS OF HUMAN GAMMA-GLOBULIN. Immunology. 1965 Feb;8:206–212. [PMC free article] [PubMed] [Google Scholar]
  22. Morgan A. R., Wells R. D., Khorana H. G. Studies on polynucleotides, lix. Further codon assignments from amino Acid incorporations directed by ribopolynucleotides containing repeating trinucleotide sequences. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1899–1906. doi: 10.1073/pnas.56.6.1899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mårtensson L. Genes and immunoglobulins. Vox Sang. 1966 Sep-Oct;11(5):521–545. doi: 10.1111/j.1423-0410.1966.tb04251.x. [DOI] [PubMed] [Google Scholar]
  24. Natvig J. B. Gm(g)--a "new" gamma-globulin factor. Nature. 1966 Jul 16;211(5046):318–319. doi: 10.1038/211318a0. [DOI] [PubMed] [Google Scholar]
  25. OSSERMAN E. F., TAKATSUKI K. CLINICAL AND IMMUNOCHEMICAL STUDIES OF FOUR CASES OF HEAVY (H-GAMMA-2) CHAIN DISEASE. Am J Med. 1964 Sep;37:351–373. doi: 10.1016/0002-9343(64)90192-5. [DOI] [PubMed] [Google Scholar]
  26. PAIN R. H. THE MOLECULAR WEIGHTS OF THE PEPTIDE CHAINS OF GAMMA-GLOBULIN. Biochem J. 1963 Aug;88:234–239. doi: 10.1042/bj0880234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. POTTS J. T., Jr, YOUNG D. M., ANFINSEN C. B., SANDOVAL A. STUDIES ON RIBONUCLEASE S. I. LIMITED CARBOXYPEPTIDASE DEGRADATION OF RIBONUCLEASE S-PROTEIN AND RIBONUCLEASE S-PEPTIDE: EFFECTS OF CHANGES IN PRIMARY STRUCTURE ON ENZYMIC ACTIVITY. J Biol Chem. 1964 Nov;239:3781–3786. [PubMed] [Google Scholar]
  28. Press E. M., Piggot P. J., Porter R. R. The N- and c-terminal amino acid sequences of the heavy chain from a pathological human immunoglobulin IgG. Biochem J. 1966 May;99(2):356–366. doi: 10.1042/bj0990356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. ROBERT B., DENES Y., CREPIN Y. MISE EN 'EVIDENCE DE PEPTIDES DIALYSABLES ACCOMPAGNANT LES GAMMA-GLOBULINES DANS LEUR PR'EPARATION. C R Hebd Seances Acad Sci. 1965 Jan 11;260:734–737. [PubMed] [Google Scholar]
  30. Spiegelberg H. L., Weigle W. O. Studies on the catabolism of gamma- G subunits and chains. J Immunol. 1965 Dec;95(6):1034–1040. [PubMed] [Google Scholar]
  31. TERRY W. D., FAHEY J. L. SUBCLASSES OF HUMAN GAMMA-2-GLOBULIN BASED ON DIFFERENCES IN THE HEAVY POLYPEPTIDE CHAINS. Science. 1964 Oct 16;146(3642):400–401. doi: 10.1126/science.146.3642.400. [DOI] [PubMed] [Google Scholar]
  32. Terry W. D., Fahley J. L., Steinberg A. G. GM and INV factors in subclasses of human IgG. J Exp Med. 1965 Dec 1;122(6):1087–1102. doi: 10.1084/jem.122.6.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Terry W. D. Skin-sensitizing activity related to gamma- polypeptide chain characteristics of human IgG. J Immunol. 1965 Dec;95(6):1041–1047. [PubMed] [Google Scholar]
  34. Thorpe N. O., Deutsch H. F. Studies on papain produced subunits of human gamma-G-globulins. II. Structures of peptides related to the genetic Gm activity of gamma-G-globulin Fc-fragments. Immunochemistry. 1966 Jul;3(4):329–337. [PubMed] [Google Scholar]
  35. Weir R. C., Porter R. R., Givol D. Comparison of the C-terminal amino-acid sequence of two horse immunoglobulins IgG and IgG(T). Nature. 1966 Oct 8;212(5058):205–206. doi: 10.1038/212205a0. [DOI] [PubMed] [Google Scholar]

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