Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1968 Jan;106(1):185–192. doi: 10.1042/bj1060185

The effect of adenosine triphosphate on porphyrin excretion and on glycine metabolism in Rhodopseudomonas spheroides

A Gajdos 1, M Gajdos-Török 1, A Gorchein 1, A Neuberger 1, G H Tait 1
PMCID: PMC1198485  PMID: 5721455

Abstract

1. ATP, GTP, CTP and UTP at concentrations of 1mm markedly decrease the amount of coproporphyrin excreted by Rhodopseudomonas spheroides illuminated in a medium containing glycine, succinate and fumarate. 2. The effect of ATP is decreased if ethionine is also added to the medium. 3. Evidence is presented showing that ATP is taken up by the organisms from the medium. 4. ATP is shown to have a marked effect on the utilization of glycine. In the presence of ATP the incorporation of the methylene carbon atom of glycine into the fatty acid moieties of the phospholipids is greatly increased, and more of the carboxyl carbon atom is lost, probably as carbon dioxide. 5. ATP has little effect on the utilization of succinate or fumarate. 6. The possible significance of these results with regard to the control by ATP of porphyrin synthesis and excretion and glycine metabolism is discussed.

Full text

PDF
185

Images in this article

191Fig. 3.
on p.191

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. BENSON A. A., MARUO B. Piant phospholipids. I. Identification of the phosphatidyl glycerols. Biochim Biophys Acta. 1958 Jan;27(1):189–195. doi: 10.1016/0006-3002(58)90308-1. [DOI] [PubMed] [Google Scholar]
  3. BREMER J., GREENBERG D. M. Mono- and dimethylethanolamine isolated from rat-liver phospholipids. Biochim Biophys Acta. 1959 Sep;35:287–288. doi: 10.1016/0006-3002(59)90375-0. [DOI] [PubMed] [Google Scholar]
  4. GAJDOS A., GAJDOS-TOEROEK M. INHIBITORY ACTION OF ADENINE, ADENINENUCLEOSIDES AND NUCLEOTIDES ON THE BIOSYNTHESIS OF PORPHYRINS BY RHODOPSEUDOMONASSPHEROIDES. Nature. 1963 Sep 14;199:1093–1094. doi: 10.1038/1991093a0. [DOI] [PubMed] [Google Scholar]
  5. GIBSON K. D., MATTHEW M., NEUBERGER A., TAIT G. H. Biosynthesis of porphyrins and chlorophylls. Nature. 1961 Oct 21;192:204–208. doi: 10.1038/192204a0. [DOI] [PubMed] [Google Scholar]
  6. GIBSON K. D., NEUBERGER A., TAIT G. H. Studies on the biosynthesis of porphyrin and bacteriochlorophyll by Rhodopseudomonas spheroides. 1. The effect of growth conditions. Biochem J. 1962 Jun;83:539–549. doi: 10.1042/bj0830539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GIBSON K. D., NEUBERGER A., TAIT G. H. Studies on the biosynthesis of porphyrin and bacteriochlorophyll by Rhodopseudomonas spheroides. 2. The effects of ethionine and threonine. Biochem J. 1962 Jun;83:550–559. doi: 10.1042/bj0830550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gajdos A., Gajdos-Török M., Palma-Carlos A., Palma-Carlos L. Corrélation négative entre le taux hépatique de l'ATP et celui des porphyrines chez le rat blanc intoxiqué par le sédormid. C R Seances Soc Biol Fil. 1966;160(5):953–957. [PubMed] [Google Scholar]
  9. HANDS A. R., BARTLEY W. The fatty acids of Rhodopseudomonas particles. Biochem J. 1962 Aug;84:238–239. doi: 10.1042/bj0840238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KIKUCHI G., KUMAR A., TALMAGE P., SHEMIN D. The enzymatic synthesis of delta-aminolevulinic acid. J Biol Chem. 1958 Nov;233(5):1214–1219. [PubMed] [Google Scholar]
  11. LASCELLES J. The synthesis of enzymes concerned in bacteriochlorophyll formation in growing cultures of Rhodopseudomonas spheroides. J Gen Microbiol. 1960 Dec;23:487–498. doi: 10.1099/00221287-23-3-487. [DOI] [PubMed] [Google Scholar]
  12. LASCELLES J. The synthesis of porphyrins and bacteriochlorophyll by cell suspensions of Rhodopseudomonas spheroides. Biochem J. 1956 Jan;62(1):78–93. doi: 10.1042/bj0620078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LESSIE T. G., SISTROM W. R. CONTROL OF PORPHYRIN SYNTHESIS IN RHODOPSEUMOMONAS SPHEROIDES. Biochim Biophys Acta. 1964 May 11;86:250–259. doi: 10.1016/0304-4165(64)90050-9. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Nandi D. L., Waygood E. R. Biosynthesis of porphyrins in wheat leaves. II. 5-aminolaevulinate hydro-lyase. Can J Biochem. 1967 Feb;45(2):327–336. doi: 10.1139/o67-036. [DOI] [PubMed] [Google Scholar]
  16. SISTROM W. R. Observations on the relationship between the formation of photopigments and the synthesis of protein in Rhodopseudomonas spheroides. J Gen Microbiol. 1962 Sep;28:599–605. doi: 10.1099/00221287-28-4-599. [DOI] [PubMed] [Google Scholar]
  17. STREHLER B. L., TOTTER J. R. Determination of ATP and related compounds: firefly luminescence and other methods. Methods Biochem Anal. 1954;1:341–356. doi: 10.1002/9780470110171.ch13. [DOI] [PubMed] [Google Scholar]
  18. Shull K. H., McConomy J., Vogt M., Castillo A., Farber E. On the mechanism of induction of hepatic adenosine triphosphate deficiency by ethionine. J Biol Chem. 1966 Nov 10;241(21):5060–5070. [PubMed] [Google Scholar]
  19. TSUIKI S., KIKUCHI G. Catabolism of glycine in Rhodopseudomonas spheroides. Biochim Biophys Acta. 1962 Nov 5;64:514–525. doi: 10.1016/0006-3002(62)90310-4. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES