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. 1968 Jan;106(2):361–366. doi: 10.1042/bj1060361

C-phycocyanin and allophycocyanin in two species of blue-green algae

I W Craig 1, N G Carr 1
PMCID: PMC1198511  PMID: 5637347

Abstract

1. The biliproteins C-phycocyanin and allophycocyanin were purified from the blue–green alga Anabaena variabilis by ammonium sulphate fractionation and gel filtration. 2. An assay procedure that enabled the proportion of the two pigments, present as a mixture, to be determined was devised by using the data provided by spectrophotometric analysis of the purified biliproteins. 3. The degree of association and relative proportions of the two pigments were analysed by the application of this procedure to the components separated by thin-layer gel filtration. 4. The C-phycocyanin/allophycocyanin ratio remained essentially constant in algal extracts prepared at various stages throughout the growth cycle or after growth under conditions of reduced illumination. 5. The behaviour of the C-phycocyanin aggregate species from Anacystis nidulans suggested that they were of appreciably lower molecular weight than those observed in extracts of Anabaena variabilis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNS D. S., SCOTT E., O'REILLY K. T. C-PHYCOCYANIN: MINIMUM MOLECULAR WEIGHT. Science. 1964 Sep 4;145(3636):1054–1056. doi: 10.1126/science.145.3636.1054. [DOI] [PubMed] [Google Scholar]
  2. Berns D. S., Edwards M. R. Electron micrographic investigations of C-phycocyanin. Arch Biochem Biophys. 1965 Jun;110(3):511–516. doi: 10.1016/0003-9861(65)90444-3. [DOI] [PubMed] [Google Scholar]
  3. Berns D. S., Morgenstern A. Ultracentrifuge investigation of protein aggregation in dilute solutions of C-phycocyanin. Biochemistry. 1966 Sep;5(9):2985–2990. doi: 10.1021/bi00873a030. [DOI] [PubMed] [Google Scholar]
  4. Bucke C., Leech R. M., Hallaway M., Morton R. A. The taxonomic distribution of plastoquinone and tocopherolquinone and their intracellular distribution in leaves of Vicia faba L. Biochim Biophys Acta. 1966 Jan 4;112(1):19–34. doi: 10.1016/s0926-6585(96)90004-7. [DOI] [PubMed] [Google Scholar]
  5. Carr N. G., Hallaway M. Reduction of phenolindo-2,6-dichlorophenol in dark and light by the blue-green alga, Anabaena variabilis. J Gen Microbiol. 1965 Jun;39(3):335–344. doi: 10.1099/00221287-39-3-335. [DOI] [PubMed] [Google Scholar]
  6. Forrest H. S., VAN Baalen C., Myers J. Occurrence of Pteridines in a Blue-Green Alga. Science. 1957 Apr 12;125(3250):699–700. doi: 10.1126/science.125.3250.699. [DOI] [PubMed] [Google Scholar]
  7. Fujimori E., Pecci J. Dissociation and association of phycocyanin. Biochemistry. 1966 Nov;5(11):3500–3508. doi: 10.1021/bi00875a016. [DOI] [PubMed] [Google Scholar]
  8. HAXO F., O'HEOCHA C., NORRIS P. Comparative studies of chromatographically separated phycoerythrins and phycocyanins. Arch Biochem Biophys. 1955 Jan;54(1):162–173. doi: 10.1016/0003-9861(55)90019-9. [DOI] [PubMed] [Google Scholar]
  9. Hattori A., Crespi H. L., Katz J. J. Effect of side-chain deuteration on protein stability. Biochemistry. 1965 Jul;4(7):1213–1225. doi: 10.1021/bi00883a002. [DOI] [PubMed] [Google Scholar]
  10. SWINGLE S. M., TISELIUS A. Tricalcium phosphate as an adsorbent in the chromatography of proteins. Biochem J. 1951 Feb;48(2):171–174. doi: 10.1042/bj0480171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Scott E., Berns D. S. Protein-protein interaction. The phycocyanin system. Biochemistry. 1965 Dec;4(12):2597–2606. doi: 10.1021/bi00888a008. [DOI] [PubMed] [Google Scholar]

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