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. 1968 Feb;106(3):587–591. doi: 10.1042/bj1060587

The submicrosomal distribution of hepatic uridine diphosphate glucuronyltransferases in the rabbit

Theodore E Gram 1,*, Alfred R Hansen 1, James R Fouts 1
PMCID: PMC1198546  PMID: 5639914

Abstract

1. Rabbit liver microsomes were subfractionated into rough- and smooth-surfaced types, and glucuronyltransferase activity in each microsomal subfraction was determined with p-nitrophenol, o-aminophenol and phenolphthalein as substrates. The glucuronyltransferase activity measured with p-nitrophenol and o-aminophenol as substrates was localized predominantly in rough-surfaced microsomes. Glucuronyltransferase measured with phenolphthalein as substrate was equally present in rough- and smooth-surfaced microsomes. 2. Phenobarbital pretreatment of rabbits did not stimulate any of the glucuronyltransferase activities measured in either rough- or smooth-surfaced microsomes. 3. Preincubation of rabbit liver microsomes for 30–60min. at 37° under oxygen did not cause any loss of glucuronyltransferase activity. Such preincubation caused either no change or increased enzyme activity in both submicrosomal fractions. The relative distribution of transferase activity in rough- and smooth-surfaced microsomes was not affected by preincubation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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