Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1968 Feb;106(3):749–757. doi: 10.1042/bj1060749

The purification and amino acid composition of human uterus collagens, rheumatoid-arthritis-nodule collagen and ox tendon collagen

J J Harding 1,*, J M Wesley 1
PMCID: PMC1198568  PMID: 5639930

Abstract

Collagens and gelatins were isolated from human post-menopausal uterus, puerperal (post-partum) uterus, rheumatoid-arthritis-nodule and ox tendon. Different means of purifying collagen were studied and a method was devised that enables highly purified collagen to be obtained, even from the uterus. This method involves the use of a number of aqueous and organic extractants as well as digestion with elastase to eliminate elastin. The purity of the collagen preparations was assessed and they were used to study the amino acid composition of collagen. The amino acid compositions of all the collagens studied were similar to those of human bone and tendon collagen, but certain small differences were noted and are discussed. The soluble collagen extracted from some of the tissues was also studied.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anwar R. A. Comparison of elastins from various sources. Can J Biochem. 1966 Jun;44(6):725–734. doi: 10.1139/o66-090. [DOI] [PubMed] [Google Scholar]
  2. BROWN P. C., CONSDEN R., GLYNN L. E. Observations on the shrink temperature of collagen and its variations with age and disease. Ann Rheum Dis. 1958 Jun;17(2):196–208. doi: 10.1136/ard.17.2.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CONSDEN R., GLYNN L. E., STANIER W. M. A chemical examination of connective tissue in rheumatic fever. Biochem J. 1953 Sep;55(2):248–253. doi: 10.1042/bj0550248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chibnall A. C., Rees M. W., Williams E. F. The total nitrogen content of egg albumin and other proteins. Biochem J. 1943 Sep;37(3):354–359. doi: 10.1042/bj0370354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Consden R., Kirrane J. A. Action of ultra-violet light on soluble collagens. Nature. 1967 Jul 8;215(5097):165–166. doi: 10.1038/215165a0. [DOI] [PubMed] [Google Scholar]
  6. EASTOE J. E., EASTOE B. The organic constituents of mammalian compact bone. Biochem J. 1954 Jul;57(3):453–459. doi: 10.1042/bj0570453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. EASTOE J. E. The amino acid composition of mammalian collagen and gelatin. Biochem J. 1955 Dec;61(4):589–600. doi: 10.1042/bj0610589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FRANCOIS C., MARSHALL R. D., NEUBERGER A. Carbohydrates in protein. 4. The determination of mannose in hen's-egg albumin by radioisotope dilution. Biochem J. 1962 May;83:335–341. doi: 10.1042/bj0830335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HAMOIR G., KONOSU S. CARP MYOGENS OF WHITE AND RED MUSCLES. GENERAL COMPOSITION AND ISOLATION OF LOW-MOLECULAR-WEIGHT COMPONENTS OF ABNORMAL AMINO ACID COMPOSITION. Biochem J. 1965 Jul;96:85–97. doi: 10.1042/bj0960085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HARDING J. J. The amino acid composition of human collagens from adult dura mater and post-menopausal uterus. Biochem J. 1963 Mar;86:574–576. doi: 10.1042/bj0860574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HARKNESS M. L., HARKNESS R. D. The collagen content of the reproductive tract of the rat during pregnancy and lactation. J Physiol. 1954 Mar 29;123(3):492–500. doi: 10.1113/jphysiol.1954.sp005066. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HARKNESS R. D., MORALEE B. E. The time-course and route of loss of collagen from the rat's uterus during post-partum involution. J Physiol. 1956 Jun 28;132(3):502–508. doi: 10.1113/jphysiol.1956.sp005543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HARTSHORNE D. J., PERRY S. V. A chromatographic and electrophoretic study of sarcoplasm from adult--and foetal-rabbit muscles. Biochem J. 1962 Oct;85:171–177. doi: 10.1042/bj0850171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Harding J. J. The unusual links and cross-links of collagen. Adv Protein Chem. 1965;20:109–190. doi: 10.1016/s0065-3233(08)60389-7. [DOI] [PubMed] [Google Scholar]
  15. KRONMAN M. J., WEINBERGER L. E., WINTERBOTTOM R. J. The water-soluble proteins of bovine skeletal muscle. Arch Biochem Biophys. 1960 Feb;86:238–250. doi: 10.1016/0003-9861(60)90412-4. [DOI] [PubMed] [Google Scholar]
  16. LASZT L., HAMOIR G. [Study by electrophoresis and ultracentrifugation of the protein composition of the muscular coat of bovine carotid arteries]. Biochim Biophys Acta. 1961 Jul 8;50:430–449. doi: 10.1016/0006-3002(61)90002-6. [DOI] [PubMed] [Google Scholar]
  17. LEACH A. A. The amino acid composition of amphibian, reptile and avian gelatins. Biochem J. 1957 Sep;67(1):83–87. doi: 10.1042/bj0670083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MONTFORT I., PEREZ-TAMAYO R. Studies on uterine collagen during pregnancy and puerperium. Lab Invest. 1961 Nov-Dec;10:1240–1258. [PubMed] [Google Scholar]
  19. MORRIONE T. G., SEIFTER S. Alteration in the collagen content of the human uterus during pregnancy and post partum involution. J Exp Med. 1962 Feb 1;115:357–365. doi: 10.1084/jem.115.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PIEZ K. A., GROSS J. The amino acid composition of some fish collagens: the relation between composition and structure. J Biol Chem. 1960 Apr;235:995–998. [PubMed] [Google Scholar]
  21. PIEZ K. A., MORRIS L. A modified procedure for the automatic analysis of amino acids. Anal Biochem. 1960 Nov;1:187–201. doi: 10.1016/0003-2697(60)90045-2. [DOI] [PubMed] [Google Scholar]
  22. RUBIN A. L., PFAHL D., SPEAKMAN P. T., DAVISON P. F., SCHMITT F. O. Tropocollagen: significance of protease-induced alterations. Science. 1963 Jan 4;139(3549):37–39. doi: 10.1126/science.139.3549.37. [DOI] [PubMed] [Google Scholar]
  23. SCHAUB M. C. DEGRADATION OF YOUNG AND OLD COLLAGEN BY EXTRACTS OF VARIOUS ORGANS. Gerontologia. 1964;49:52–60. doi: 10.1159/000211236. [DOI] [PubMed] [Google Scholar]
  24. SCHAUB M. C. EIGENSCHAFTEN UND INTRACELLULAERE VERTEILUNG EINES KOLLAGENABBAUENDEN KATHEPSINS. Helv Physiol Pharmacol Acta. 1964;22:271–284. [PubMed] [Google Scholar]
  25. SKEGGS L. T., Jr An automatic method for colorimetric analysis. Am J Clin Pathol. 1957 Sep;28(3):311–322. doi: 10.1093/ajcp/28.3_ts.311. [DOI] [PubMed] [Google Scholar]
  26. Schaub M. C. Die Wirkung von Kathepsin auf Kollagen und Casein im graviden und nicht-graviden Uterus der Ratte. Hoppe Seylers Z Physiol Chem. 1965;343(1):130–134. [PubMed] [Google Scholar]
  27. Steven F. S., Jackson D. S. Purification and amino acid composition of monomeric and polymeric collagens. Biochem J. 1967 Aug;104(2):534–536. doi: 10.1042/bj1040534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. WINDRUM G. M., KENT P. W., EASTOE J. E. The constitution of human renal reticulin. Br J Exp Pathol. 1955 Feb;36(1):49–59. [PMC free article] [PubMed] [Google Scholar]
  29. WINZLER R. J. Determination of serum glycoproteins. Methods Biochem Anal. 1955;2:279–311. doi: 10.1002/9780470110188.ch10. [DOI] [PubMed] [Google Scholar]
  30. WOESSNER J. F., BREWER T. H. FORMATION AND BREAKDOWN OF COLLAGEN AND ELASTIN IN THE HUMAN UTERUS DURING PREGNANCY AND POST-PARTUM INVOLUTION. Biochem J. 1963 Oct;89:75–82. doi: 10.1042/bj0890075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. WOESSNER J. F., Jr Catabolism of collagen and non-collagen protein in the rat uterus during post-partum involution. Biochem J. 1962 May;83:304–314. doi: 10.1042/bj0830304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Woessner J. F., Jr Acid hydrolases of the rat uterus in relation to pregnancy, post-partum involution and collagen breakdown. Biochem J. 1965 Dec;97(3):855–866. doi: 10.1042/bj0970855. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES