Fig. 2 Knockout of OsRFI2 enhances salt tolerance of rice plants. (A) Phenotypes of WT, and Osrfi2, OE-OsRFI2 transgenic lines under control and salt treatment. The 21-d-old seedlings of WT, Osrfi2, and OE-OsRFI2 were hydroponically cultivated in Yoshida’s nutrient solution with or without 120 mM NaCl and treated for 7 days. The assays are performed by more than three independent experiments. Bar = 1 cm. (B-G) Measurements of the leaf withered rate (B), relative fresh weight (C), total chlorophyll content (D), H₂O₂ content (E), O₂⁻ content (F), and MDA content (G) of WT, Osrfi2, and OE-OsRFI2 lines. Mean ± S.D. values are calculated from more than 10 seedlings each of three independent experiments, and the different letters on the columns indicate statistically significant differences (Duncan’s test, P < 0.05)