Correction: E3 Ubiquitin Ligase OsRFI2 Regulates Salinity Tolerance by Targeting Ascorbate Peroxidase OsAPX8 for its Degradation in Rice

Correction: Rice (2025) 18:12 10.1186/s12284-025-00763-x

In this article, Figs. 2 and 3 appeared incorrectly and have now been corrected in the original publication. For completeness and transparency, the incorrect and correct versions of Figs. 2 and 3 are displayed below.

Fig. 2 Knockout of OsRFI2 enhances salt tolerance of rice plants. (A) Phenotypes of WT, and Osrfi2, OE-OsRFI2 transgenic lines under control and salt treatment. The 21-d-old seedlings of WT, Osrfi2, and OE-OsRFI2 were hydroponically cultivated in Yoshida’s nutrient solution with or without 120 mM NaCl and treated for 7 days. The assays are performed by more than three independent experiments. Bar = 1 cm. (B-G) Measurements of the leaf withered rate (B), relative fresh weight (C), total chlorophyll content (D), H₂O₂ content (E), O₂⁻ content (F), and MDA content (G) of WT, Osrfi2, and OE-OsRFI2 lines. Mean ± S.D. values are calculated from more than 10 seedlings each of three independent experiments, and the different letters on the columns indicate statistically significant differences (Duncan’s test, P < 0.05)

Fig. 3 OsRFI2 interacts with OsAPX8. (A) Interaction between OsRFI2 and OsAPX8 verified by Y2H assay. OsRFI2 and OsAPX8 were cloned into pGADT7 and pGBKT7, respectively. Yeast cells were grown on SD/-Leu/-Trp and SD/-His/-Leu/-Trp + AbA synthetic dropout medium, respectively. BD, bait vector pGBKT7; AD, prey vector pGADT7. pGBKT7-p53 + pGADT7-T was used as a positive control. (B) GST pull-down assay for interaction between OsRFI2 and OsAPX8. OsRFI2-GST was used as the bait to pull down OsAPX8-His. The GST alone was used as control. An equal amount of OsAPX8-His was used in pulldown assays and as the input control. (C) BiFC visualization of the interaction between OsRFI2 and OsAPX8 in N. benthamiana leaves. The images were taken under a confocal microscope after transfection for 60 h. The combinations of empty vector cGFP with nGFP-OsRFI2 and empty vector nGFP with OsAPX8-cGFP served as negative controls, respectively. Bar = 1 mm

Incorrect Fig. 2

Fig. 2 Knockout of OsRFI2 enhances salt tolerance of rice plants. (A) Phenotypes of WT, and Osrfi2, OE-OsRFI2 transgenic lines under control and salt treatment. The 21-d-old seedlings of WT, Osrfi2, and OE-OsRFI2 were hydroponically cultivated in Yoshida’s nutrient solution with or without 120 mM NaCl and treated for 7 days. The assays are performed by more than three independent experiments. Bar = 1 cm. (B-G) Measurements of the leaf withered rate (B), relative fresh weight (C), total chlorophyll content (D), H₂O₂ content (E), O₂⁻ content (F), and MDA content (G) of WT, Osrfi2, and OE-OsRFI2 lines. Mean ± S.D. values are calculated from more than 10 seedlings each of three independent experiments, and the different letters on the columns indicate statistically significant differences (Duncan’s test, P < 0.05)

Correct Fig. 2

Incorrect Fig. 3

Fig. 3 OsRFI2 interacts with OsAPX8. (A) Interaction between OsRFI2 and OsAPX8 verified by Y2H assay. OsRFI2 and OsAPX8 were cloned into pGADT7 and pGBKT7, respectively. Yeast cells were grown on SD/-Leu/-Trp and SD/-His/-Leu/-Trp + AbA synthetic dropout medium, respectively. BD, bait vector pGBKT7; AD, prey vector pGADT7. pGBKT7-p53 + pGADT7-T was used as a positive control. (B) GST pull-down assay for interaction between OsRFI2 and OsAPX8. OsRFI2-GST was used as the bait to pull down OsAPX8-His. The GST alone was used as control. An equal amount of OsAPX8-His was used in pulldown assays and as the input control. (C) BiFC visualization of the interaction between OsRFI2 and OsAPX8 in N. benthamiana leaves. The images were taken under a confocal microscope after transfection for 60 h. The combinations of empty vector cGFP with nGFP-OsRFI2 and empty vector nGFP with OsAPX8-cGFP served as negative controls, respectively. Bar = 1 mm

Correct Fig. 3

The original article has been corrected.