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. 2025 Apr 11;18:26. doi: 10.1186/s12284-025-00780-w

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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graphic file with name 12284_2025_780_Fig3_HTML.jpg — Fig. 3 OsRFI2 interacts with OsAPX8. (A) Interaction between OsRFI2 and OsAPX8 verified by Y2H assay. OsRFI2 and OsAPX8 were cloned into pGADT7 and pGBKT7, respectively. Yeast cells were grown on SD/-Leu/-Trp and SD/-His/-Leu/-Trp + AbA synthetic dropout medium, respectively. BD, bait vector pGBKT7; AD, prey vector pGADT7. pGBKT7-p53 + pGADT7-T was used as a positive control. (B) GST pull-down assay for interaction between OsRFI2 and OsAPX8. OsRFI2-GST was used as the bait to pull down OsAPX8-His. The GST alone was used as control. An equal amount of OsAPX8-His was used in pulldown assays and as the input control. (C) BiFC visualization of the interaction between OsRFI2 and OsAPX8 in N. benthamiana leaves. The images were taken under a confocal microscope after transfection for 60 h. The combinations of empty vector cGFP with nGFP-OsRFI2 and empty vector nGFP with OsAPX8-cGFP served as negative controls, respectively. Bar = 1 mm