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. 1968 Apr;107(4):507–518. doi: 10.1042/bj1070507

Determination of bilirubin glucuronide and assay of glucuronyltransferase with bilirubin as acceptor

F P Van Roy 1,*, K P M Heirwegh 1
PMCID: PMC1198694  PMID: 5660631

Abstract

1. Conjugated bilirubin is conveniently determined by coupling with the diazonium salt of ethyl anthranilate. 2. This method has been used in the development of assays for UDP-glucuronyltransferase (EC 2.4.1.17), with bilirubin as substrate, in rat liver homogenates, microsomal preparations and partly purified fractions. 3. Chromatographic analysis suggests that bilirubin monoglucuronide is the product of the enzyme systems studied.

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Selected References

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