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. 1968 Jul;108(3):437–444. doi: 10.1042/bj1080437

Correlated changes of some enzyme activities and cofactor and substrate contents of pea cotyledon tissue during germination

A P Brown 1, J L Wray 1,*
PMCID: PMC1198829  PMID: 4385939

Abstract

1. The activities of six enzymes (hexokinase, phosphoglucose isomerase, phosphofructokinase, aldolase, glucose 6-phosphate dehydrogenase and amylase) in extracts of pea cotyledons were determined. The activities during the first 10 days after germination showed individual and characteristic changes that indicate a specific control of both synthesis and destruction of enzymes. 2. Tissue contents of glucose, inorganic phosphate, glucose 6-phosphate, fructose 6-phosphate, ATP, ADP, AMP, NAD and NADP were also determined, and a correlation is reported between the substrate concentrations at day 1 and the subsequent enzymic activity. 3. The initial NAD+/NADH ratio value of 1 changed to about 3 by day 4; the NADP content was lower and changes in the oxidation state were less striking. The ratio of ATP to ADP and AMP remained virtually constant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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