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. 1968 Jul;108(4):611–618. doi: 10.1042/bj1080611

The preparation and properties of purified 125I-labelled antibodies to insulin

L E M Miles 1, C N Hales 1
PMCID: PMC1198858  PMID: 5667273

Abstract

1. A procedure is described for the preparation of an insulin-immunoadsorbent containing 105–185mg. of insulin/g. of matrix, and with an antibody-binding capacity of 660mg./g. of insulin-immunoadsorbent. 2. Purified non-precipitating 125I-labelled antibodies were prepared by iodination of the antibodies while they were isolated on the insulin-immunoadsorbent in order to protect at least one antigen-binding site. 3. The radioactive antibody was simply and rapidly separated from other radioactive material and damaging reagents, and found to be up to 94% pure as judged by its reaction with insulin-immunoadsorbent and unfixed insulin. 4. The 125I-labelled antibody preparations had iodine contents of 0·5–64atoms/mol. of protein and specific radioactivities of 0·3–125mc/mg. of protein. 5. It is suggested that this is a simple method of producing a useful reagent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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