Abstract
1. A method is described for measuring the concentration of periodate over the range 0·2–20μm by adding 1,2-di-(p-dimethylaminophenyl)ethane-1,2-diol to a sample solution. Periodate cleaves this compound to from two molecules of p-dimethylaminobenzaldehyde, the extinction of which is then read at 352mμ. 2. The method has been used to follow the course of periodate oxidations of serine methyl ester, ribonuclease A and ribonuclease S-protein. Addition of the reagent stops further periodate reaction by reducing the remaining periodate to iodate. 3. The presence of protein does not interfere with the assay.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Doscher M. S., Hirs C. H. The heterogeneity of bovine pancreatic ribonuclease S. Biochemistry. 1967 Jan;6(1):304–312. doi: 10.1021/bi00853a047. [DOI] [PubMed] [Google Scholar]
- RICHARDS F. M., VITHAYATHIL P. J. The preparation of subtilisn-modified ribonuclease and the separation of the peptide and protein components. J Biol Chem. 1959 Jun;234(6):1459–1465. [PubMed] [Google Scholar]