Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1983 Jun;339:663–678. doi: 10.1113/jphysiol.1983.sp014741

The charge carried by single-channel currents of rat cultured muscle cells in the presence of local anaesthetics.

E Neher
PMCID: PMC1199186  PMID: 6310093

Abstract

Acetylcholine-induced single-channel currents were measured in the presence of the lignocaine derivative QX222. Unit responses appeared as bursts of short current pulses as a result of the fast blocking action of the drug (QX222). The amplitude of the individual current pulses was not changed by the presence of the drug up to a concentration of 250 microM. The time integral of current during a burst, which for a sequential blocking model should be independent of drug concentration, decreased at concentrations of QX222 higher than 40 microM. The distribution of gap times within a burst could not be fitted by a single exponential for high concentrations of QX222. It is concluded that the simple sequential model of channel blockade does not apply for concentrations of QX222 higher than 40 microM.

Full text

PDF
663

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams P. R. Drug blockade of open end-plate channels. J Physiol. 1976 Sep;260(3):531–552. doi: 10.1113/jphysiol.1976.sp011530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adams P. R., Feltz A. End-plate channel opening and the kinetics of quinacrine (mepacrine) block. J Physiol. 1980 Sep;306:283–306. doi: 10.1113/jphysiol.1980.sp013397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adams P. R. Voltage jump analysis of procaine action at frog end-plate. J Physiol. 1977 Jun;268(2):291–318. doi: 10.1113/jphysiol.1977.sp011858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ascher P., Marty A., Neild T. O. The mode of action of antagonists of the excitatory response to acetylcholine in Aplysia neurones. J Physiol. 1978 May;278:207–235. doi: 10.1113/jphysiol.1978.sp012300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beam K. G. A quantitative description of end-plate currents in the presence of two lidocaine derivatives. J Physiol. 1976 Jun;258(2):301–322. doi: 10.1113/jphysiol.1976.sp011421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Colquhoun D., Hawkes A. G. On the stochastic properties of bursts of single ion channel openings and of clusters of bursts. Philos Trans R Soc Lond B Biol Sci. 1982 Dec 24;300(1098):1–59. doi: 10.1098/rstb.1982.0156. [DOI] [PubMed] [Google Scholar]
  7. Colquhoun D., Hawkes A. G. Relaxation and fluctuations of membrane currents that flow through drug-operated channels. Proc R Soc Lond B Biol Sci. 1977 Nov 14;199(1135):231–262. doi: 10.1098/rspb.1977.0137. [DOI] [PubMed] [Google Scholar]
  8. Colquhoun D., Sakmann B. Fluctuations in the microsecond time range of the current through single acetylcholine receptor ion channels. Nature. 1981 Dec 3;294(5840):464–466. doi: 10.1038/294464a0. [DOI] [PubMed] [Google Scholar]
  9. Colquhoun D., Sheridan R. E. The modes of action of gallamine. Proc R Soc Lond B Biol Sci. 1981 Mar 6;211(1183):181–203. doi: 10.1098/rspb.1981.0002. [DOI] [PubMed] [Google Scholar]
  10. Farley J. M., Yeh J. Z., Watanabe S., Narahashi T. Endplate channel block by guanidine derivatives. J Gen Physiol. 1981 Mar;77(3):273–293. doi: 10.1085/jgp.77.3.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gage P. W., Hamill O. P. Lifetime and conductance of acetylcholine-activated channels in normal and denervated toad sartorius muscle. J Physiol. 1980 Jan;298:525–538. doi: 10.1113/jphysiol.1980.sp013099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gardner D. Time integral of synaptic conductance. J Physiol. 1980 Jul;304:181–191. doi: 10.1113/jphysiol.1980.sp013318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. doi: 10.1007/BF00656997. [DOI] [PubMed] [Google Scholar]
  14. Hille B. Local anesthetics: hydrophilic and hydrophobic pathways for the drug-receptor reaction. J Gen Physiol. 1977 Apr;69(4):497–515. doi: 10.1085/jgp.69.4.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Magleby K. L., Stevens C. F. A quantitative description of end-plate currents. J Physiol. 1972 May;223(1):173–197. doi: 10.1113/jphysiol.1972.sp009840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Neher E., Sakmann B. Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature. 1976 Apr 29;260(5554):799–802. doi: 10.1038/260799a0. [DOI] [PubMed] [Google Scholar]
  17. Neher E., Steinbach J. H. Local anaesthetics transiently block currents through single acetylcholine-receptor channels. J Physiol. 1978 Apr;277:153–176. doi: 10.1113/jphysiol.1978.sp012267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ogden D. C., Siegelbaum S. A., Colquhoun D. Block of acetylcholine-activated ion channels by an uncharged local anaesthetic. Nature. 1981 Feb 12;289(5798):596–598. doi: 10.1038/289596a0. [DOI] [PubMed] [Google Scholar]
  19. Ruff R. L. A quantitative analysis of local anaesthetic alteration of miniature end-plate currents and end-plate current fluctuations. J Physiol. 1977 Jan;264(1):89–124. doi: 10.1113/jphysiol.1977.sp011659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ruff R. L. The kinetics of local anesthetic blockade of end-plate channels. Biophys J. 1982 Mar;37(3):625–631. [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES