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. 1984 Jan;346:341–352. doi: 10.1113/jphysiol.1984.sp015026

Glycogen depletion elicited in tenuissimus intrafusal muscle fibres by stimulation of static gamma-axons in the cat.

L Decorte, F Emonet-Dénand, D W Harker, L Jami, Y Laporte
PMCID: PMC1199503  PMID: 6230439

Abstract

In this study the experimental conditions used to elicit glycogen depletion in tenuissimus intrafusal muscle fibres were different from those used by Barker, Emonet-Dénand, Harker, Jami & Laporte (1976): the tenuissimus was left in situ; several (4-6) static gamma-axons were stimulated together; the blood flow through the muscle was not reduced during the periods of gamma stimulation except in two experiments; very much longer periods (up to 9 h) of intermittent stimulation by bursts at 50-500/s were used. Bag1 and bag2 fibres were identified by their different ATPase activities in the B region. In two experiments with normal circulation, test responses of several primary endings to short periods of stimulation at 50-100/s were still very strong after stimulation of several static gamma-axons for 5 and 9 h, respectively. Glycogen depletion was observed in a large number of chain and bag2 poles but in only one of nineteen bag1 poles examined. In two other experiments with normal circulation, there was a very pronounced reduction of the test responses after stimulation of several static gamma-axons for 7 and 9 h, respectively. Out of twenty-four bag1 poles examined, nineteen exhibited zones of depletion. In an experiment in which stimulation was conducted as in Barker et al. (1976), i.e. with reduction of muscle blood flow during 1 min periods of stimulation at 50-100/s, the primary endings still gave a strong response after fifteen periods of stimulation in contrast with the marked 'fatigue' that was constantly observed in the former study. No depleted intrafusal fibres were found in the spindles of this muscle. In a last experiment, after an initial pattern of stimulation similar to that described above, the new pattern of stimulation, but with periodical reduction of blood flow, was applied, leading to a 'fatigue' of the test responses in 2 h. In the spindles of this muscle three out of ten bag1 poles were depleted. The variability of glycogen depletion in bag1 fibres appears to be linked to the degree of spindle 'fatigue' which may develop after static gamma stimulation. It seems that in 'fatigued' spindles some factor or factors liberated by the contraction of neighbouring fibres may deplete glycogen in bag1 fibres by a non-neural mechanism. When, in spite of a prolonged stimulation of static gamma-axons, no fatigue of the test responses develops, zones of depletion in bag1 fibres--possibly of neural origin--are very rare, although a large proportion of bag2 and chain fibres are depleted.

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Selected References

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  1. Arbuthnott E. R., Ballard K. J., Boyd I. A., Gladden M. H., Sutherland F. I. The ultrastructure of cat fusimotor endings and their relationship to foci of sarcomere convergence in intrafusal fibres. J Physiol. 1982 Oct;331:285–309. doi: 10.1113/jphysiol.1982.sp014373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Banks R. W. A histological study of the motor innervation of the cat's muscle spindle. J Anat. 1981 Dec;133(Pt 4):571–591. [PMC free article] [PubMed] [Google Scholar]
  3. Banks R. W., Barker D., Bessou P., Pagès B., Stacey M. J. Histological analysis of cat muscle spindles following direct observation of the effects of stimulating dynamic and static motor axons. J Physiol. 1978 Oct;283:605–619. doi: 10.1113/jphysiol.1978.sp012522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Banks R. W., Harker D. W., Stacey M. J. A study of mammalian intrafusal muscle fibres using a combined histochemical and ultrastructural technique. J Anat. 1977 Jul;123(Pt 3):783–796. [PMC free article] [PubMed] [Google Scholar]
  5. Barker D., Banks R. W., Harker D. W., Milburn A., Stacey M. J. Studies of the histochemistry, ultrastructure, motor innervation, and regeneration of mammalian intrafusal muscle fibres. Prog Brain Res. 1976;44:67–88. doi: 10.1016/s0079-6123(08)60724-4. [DOI] [PubMed] [Google Scholar]
  6. Barker D., Bessou P., Jankowska E., Pagès B., Stacey M. J. Identification of intrafusal muscle fibres activated by single fusimotor axons and injected with fluorescent dye in cat tenuissimus spindles. J Physiol. 1978 Feb;275:149–165. doi: 10.1113/jphysiol.1978.sp012182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Barker D., Emonet-Dénand F., Harker D. W., Jami L., Laporte Y. Distribution of fusimotor axons to intrafusal muscle fibres in cat tenuissimus spindles as determined by the glycogen-depletion method. J Physiol. 1976 Sep;261(1):49–69. doi: 10.1113/jphysiol.1976.sp011548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Barker D., Emonet-Dénand F., Laporte Y., Proske U., Stacey M. J. Morphological identification and intrafusal distribution of the endings of static fusimotor axons in the cat. J Physiol. 1973 Apr;230(2):405–427. doi: 10.1113/jphysiol.1973.sp010195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bessou P., Pagés B. Cinematographic analysis of contractile events produced in intrafusal muscle fibres by stimulation of static and dynamic fusimotor axons. J Physiol. 1975 Nov;252(2):397–427. doi: 10.1113/jphysiol.1975.sp011150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Boyd I. A., Gladden M. H., McWilliam P. N., Ward J. Control of dynamic and static nuclear bag fibres and nuclear chain fibres by gamma and beta axons in isolated cat muscle spindels. J Physiol. 1977 Feb;265(1):133–162. doi: 10.1113/jphysiol.1977.sp011709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Boyd I. A. The mechanical properties of dynamic nuclear bag fibres, static nuclear bag fibres and nuclear chain fibres in isolated cat muscle spindles. Prog Brain Res. 1976;44:33–50. [PubMed] [Google Scholar]
  12. Boyd I. A. The muscle spindle controversy. Sci Prog. 1981 Summer;67(266):205–221. [PubMed] [Google Scholar]
  13. Brooke M. H., Kaiser K. K. Three "myosin adenosine triphosphatase" systems: the nature of their pH lability and sulfhydryl dependence. J Histochem Cytochem. 1970 Sep;18(9):670–672. doi: 10.1177/18.9.670. [DOI] [PubMed] [Google Scholar]
  14. Brown M. C., Butler R. G. An investigation into the site of termination of static gamma fibres within muscle spindles of the cat peroneus longus muscle. J Physiol. 1975 May;247(1):131–143. doi: 10.1113/jphysiol.1975.sp010924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Brown M. C., Butler R. G. Studies on the site of termination of static and dynamic fusimotor fibres within muscle spindles of the tenuissimus muscle of the cat. J Physiol. 1973 Sep;233(3):553–573. doi: 10.1113/jphysiol.1973.sp010323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Edström L., Kugelberg E. Histochemical composition, distribution of fibres and fatiguability of single motor units. Anterior tibial muscle of the rat. J Neurol Neurosurg Psychiatry. 1968 Oct;31(5):424–433. doi: 10.1136/jnnp.31.5.424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Emonet-Dénand F., Jami L., Laporte Y., Tankov N. Glycogen depletion of bag1 fibers elicited by stimulation of static gamma axons in cat peroneus brevis muscle spindles. J Physiol. 1980 May;302:311–321. doi: 10.1113/jphysiol.1980.sp013244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Emonet-Dénand F., Laporte Y. Effects of prolonged stimulation at high frequency of static and dynamic gamma axons on spindle primary endings. Brain Res. 1978 Aug 11;151(3):593–598. doi: 10.1016/0006-8993(78)91092-2. [DOI] [PubMed] [Google Scholar]
  19. Gladden M. H. Structural features relative to the function of intrafusal muscle fibres in the cat. Prog Brain Res. 1976;44:51–59. doi: 10.1016/S0079-6123(08)60722-0. [DOI] [PubMed] [Google Scholar]
  20. Jami L., Lan-Couton D., Petit J. Glycogen-depletion method of intrafusal distribution of gamma-axons that increase sensitivity of spindle secondary endings. J Neurophysiol. 1980 Jan;43(1):16–26. doi: 10.1152/jn.1980.43.1.16. [DOI] [PubMed] [Google Scholar]
  21. Kucera J. The occurrence of "mixed" nuclear bag intrafusal fibers in the cat muscle spindle. Histochemistry. 1981;72(1):123–131. doi: 10.1007/BF00496787. [DOI] [PubMed] [Google Scholar]
  22. Kugelberg E. Histochemical composition, contraction speed and fatiguability of rat soleus motor units. J Neurol Sci. 1973 Oct;20(2):177–198. doi: 10.1016/0022-510x(73)90029-4. [DOI] [PubMed] [Google Scholar]
  23. Matthews P. B. Evolving views on the internal operation and functional role of the muscle spindle. J Physiol. 1981 Nov;320:1–30. doi: 10.1113/jphysiol.1981.sp013931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ovalle W. K., Smith R. S. Histochemical identification of three types of intrafusal muscle fibers in the cat and monkey based on the myosin ATPase reaction. Can J Physiol Pharmacol. 1972 Mar;50(3):195–202. doi: 10.1139/y72-030. [DOI] [PubMed] [Google Scholar]
  25. Poppele R. E., Quick D. C. Stretch-induced contraction of intrafusal muscle in cat muscle spindle. J Neurosci. 1981 Oct;1(10):1069–1074. doi: 10.1523/JNEUROSCI.01-10-01069.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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