BIOCHEMISTRY. For the article “Docosahexaenoic acid: A positive modulator of Akt signaling in neuronal survival,” by Mohammed Akbar, Frances Calderon, Zhiming Wen, and Hee-Yong Kim, which appeared in issue 31, August 2, 2005, of Proc. Natl. Acad. Sci. USA (102, 10858-10863; first published July 22, 2005; 10.1073/pnas.0502903102), the authors note that the labels “+Serine” and “-Serine” in Fig. 1C are reversed. The corrected figure and its legend appear below.
Fig. 1.
Effect of DHA on PS accumulation and apoptotic cell death. (A and B) Differential effects of DHA and DPA on PS accumulation (A) and caspase-3 activity induced by serum starvation (B). (C-E) PS-dependent inhibition of apoptosis evidenced by TUNEL positive cells (C) and representative micrographs (D), with respect to PS accumulation altered by polyunsaturated fatty acids and serine depletion (E). (F) Effect of pss1 and pss2 gene silencing on DHA-induced PS accumulation. Caspase-3 activity was expressed as the percentage to the basal value from the nonenriched control. Statistical significance was tested against nonenriched (A and F) or nonenriched serum-free control (B and C). ***, P < 0.001; **, P < 0.01; *, P < 0.05; NS, not significant.