A study focusing on the distinct fine structure of starch in mealy and waxy potatoes

Abstract

In this research, we examined the fine structure of starch in three mealy and three waxy potato varieties to understand their impact on potato texture. This research revealed significant variations in starch granule morphology, particle size, crystalline structure, molecular structure, and pasting properties between the different textures. Mealy potato starch granules appeared as rounded ovoid with inconsistent particle sizes (ranging from 35.9 to 41.8 μm), whereas waxy potato starch granules exhibited sharp polygonal shapes with consistent larger sizes (42.1–49.7 μm). While both types displayed a B-type crystalline morphology, the relative crystallinity of mealy potato starch (31.28%, 38.00% and 29.07%) is higher than that of waxy potato starch (27.38% 26.68% and 26.12%) as determined by X-ray diffraction. Additionally, the mealy potato starches had lower amylopectin content, degree of branching, short-chain amylopectin content, and molecular weight, but higher trough viscosity, final viscosity, and setback value than waxy potato starches. These differences in fine structure contributed significantly to the variations in texture between mealy and waxy potato starches, highlighting potential implications for breeding programs aimed at improving specific textural attributes for targeted application in the food industry.

Introduction

Potato (Solanum tuberosum L.) is the world’s third-largest food crop, serving as a staple food for nearly 1 billion people globally¹. Notably, China is a significant contributor to global potato production, accounting for approximately a quarter of the total yield. This role in production plays a crucial part in addressing food security challenges and mitigating regional poverty in China.

The sensory attributes of color, flavor, and texture significantly influence consumer acceptance of potatoes. The preferred taste quality serves as a primary determinant for consumers when deciding on a purchase. For instance, mealy potato varieties are favored for baked and mashed potatoes due to their dry and granular texture. These varieties also exhibit better suitability for chip production due to their lower oil absorption. Conversely, waxy potato varieties, known for their moist, gelatinous texture and good consistency, are often chosen for creamy potatoes or potato salad². The understanding of texture differences in potato varieties, food processing, home cooking, and the selection of new cultivars can greatly benefit from exploring the causes of texture differences in potatoes.

Efforts to comprehend the factors influencing texture differences in potato varieties, starch fine structure, and their impact on sensory responses have been limited. Previous studies have focused on the granular morphology of starch and cellular changes after cooking³. However, the determinants affecting potato texture remain poorly understood. While differences in dry matter content and starch properties have been explored⁴, the fine structure of starch seems to be the key factor influencing texture. Notably, limited research has focused on comparing the starch fine structure in mealy and waxy potatoes^5–8. Therefore, this study aims to systematically investigate the effect of starch fine structure on potato texture using mealy and waxy potatoes as experimental materials. The results are expected to significantly contribute to potato quality breeding.

Materials and methods

Materials

In this study, six pigmented potato resources with diverse genetic backgrounds sourced from the International Potato Center (refer to Table 1) were utilized. These resources were planted in Bainijing Village, Huize County, Yunnan Province in early April 2022, with tuber harvest taking place in late October 2022. The planting and management of the potatoes adhered to standard agronomic practices. Following harvest, the tubers were stored at 4 °C for preservation. The hardness evaluation was conducted by a panel of 9 professional tasters whose ages varied between 20 and 60 years. The hardness was rated on a 9-point scale, where 1 represented very soft and 9 represented very hard. Figure 1 provides a depiction of the texture characteristics of the potato tubers following steaming.

Table 1.

Potato resources.

Sample ID	Genotype	Skin color	Fresh color	Origin	Hardness	Texture after steaming
M1	BJ16	Red	Red	International Potato Center(CIP)	1.60	Mealy
M2	R8208	Purple	Yellow	CIP	1.30	Mealy
M3	R8219	Red	Red	CIP	1.65	Mealy
W1	BJ20	Red	Red	CIP	8.85	Waxy
W2	BJ27	Red	Red	CIP	8.73	Waxy
W3	BJ32	Yellow	Purple	CIP	8.90	Waxy

Fig. 1.

The raw potato tubers and steamed potato tubers.

Starch isolation

The protocol followed for the extraction was as mentioned in Slepkov A D et al.⁹. Potatoes were cut into small pieces and soaked in milli-Q water for 24 h at room temperature for starch extraction. The starch grains get settled at the bottom of the beaker. The top layer of the mixture is discarded and the bottom is subjected to centrifugation at 3,000 rpm at 25℃ for 10 min. The process was repeated until clear pellet is obtained and the supernatant was discarded.

Amylose content

The method for the determination of the content of amylose starch was referred to Kowsik and Mazumder¹⁰. Starch (100 mg) was weighed and mixed with 0.36 g (0.0089 mol) of NaOH in 9 mL of distilled water and 1 mL absolute ethanol. A control sample, without starch was prepared and both the flasks were heated for 10 min. After cooling, 1 mL acetic acid and 2 mL iodine solution was added. The volumes were made up to 100 mL and left for an hour at room temperature. OD was then recorded at 550 nm. The amylose content was calculated using the formula:

1

Morphology observation of starch

The surface structures of starch were obtained using a scanning electron microscope (TM4000 Plus II, Hitachi Instruments Ltd., Tokyo, Japan) according to the method described by Chao et al.¹¹.

Granule size analysis of starch

The distribution of granule size was determined using a Malvern Mastersizer 2000, with the measurement method referencing Cai et al.¹².

Degree of branching

The degree of branching of starch was measured using the AVANCE III 400 MHz NMR Spectrometer(Bruker, karlsruhe, Switzerland) according to the method of Yang et al.¹³.

Molecular weight distribution of starch

Mw was analyzed using the Agilent PL-GPC 220 high-temperature chromatograph and multiangle laser-light scatteringwith differential refractive index detector^14,15.

Chain length distribution of amylopectin

Amylopectin branch chain length distribution of starch was measured using high-performance anion exchange chromatography¹⁶. Flow rate was set as 0.5 mL/min. The elution gradient was made with 500 mM sodium acetate in 150 mM NaOH against 150 mM NaOH as follows: 0–20% for 0–5 min, 20–45% for 5–15 min, 45–60% for 15–40 min, 60–70% for 40–65 min, and 70–100% for 75–80 min.

Crystalline structure

According to the method described byGao¹⁷, the crystalline structure of starch was analyzed with X-ray diffraction (XRD) (D8, Bruker, karlsruhe, Germany).

Pasting property analysis of starch

Potato starch pasting properties were determined using a Rapid Visco Analyser (RVA, Newport Scientific, Pty Ltd., Australia). Starch (1.5 g, 12% d.b.) was mixed with 25 g of double-deionised water in the RVA sample can. The heating and cooling cycle was set as follows: (1) holding at 50℃ for 1 min, (2) heated to 95℃ in 3.8 min, (3) holding at 95 ℃ for 2.5 min, (4) cooling to 50 ℃ in 3.8 min, (5) holding at 50℃ in 1.4 min. The RVA paddle speed was at 960 rpm for the first 10 s of the test, after which the speed was at 160 rpm.

Statistical analysis

All determinations were replicated three times and the data were subjected to one-way analysis of variance and Tukey’s multiple-comparison analysis using origin2021b.

Results and discussion

Amylose content of potato starches with different texture

The amylose and amylopectin contents of various potato starch textures were analyzed and the findings are detailed in Table 2. The amylose content of the three mealy potato starch samples was determined to be 29.49%, 33.02% and 31.49% respectively, while the waxy potato starches exhibited amylose contents of 24.73%, 26.46% and 27.50% respectively. The amylose to amylopectin ratio for mealy potato starches ranged from 0.50 to 0.63, whereas the ratio for waxy potato starches ranged from 0.45 to 0.46. These results clearly demonstrate that mealy potato starches possess a higher amylose content compared to waxy potato starches.

Table 2.

Amylose content, degree of branchng and molecular weight.

Sample ID	Starch content(%)	Amylose content(%)	Amylopectin content(%)	Amylose/Amylopectin	Degree of Branching (%)	Mw(kDa)	Mn(kDa)
M1	88.61a	29.49c	59.12a	0.50c	5.16c	5396.00e	3937.90c
M2	85.47c	33.03a	52.44d	0.63a	4.78c	6612.67c	4148.40c
M3	88.27a	31.49b	56.77b	0.55b	5.99bc	5856.51de	3971.80c
Mean	87.45	31.34	59.12	0.56	5.31	5955.06	4019.37
W1	79.31d	24.73f	54.58c	0.45d	9.44a	6043.50d	4674.50ab
W2	84.63c	26.46e	58.17ab	0.45d	6.83bc	6842.50b	4737.60a
W3	86.85b	27.50d	59.34a	0.46d	8.10ab	7036.20a	4653.40ab
Mean	83.60	26.23	57.36	0.45	8.12	6640.73	4688.50

Values in the same column with different letters are significantly different (p<0.05).

Particle morphology and size distribution of potato starches with different texture

The morphology of starch granules differs based on the potato texture. Mealy potato starch granules exhibit an ovoid shape with smoth edges. In contrast waxy potato starch granules display a polygonal shape with sharp edges, consistent sizes. This suggests a correlation between starch particle morphology and potato texture. Results from particle size distribution analysis revealed that mealy potato starches had a range of 35.9–41.8 μm, whereas waxy potato starches exhibited a range of 42.1–49.7 μm, confirming the disparities in granule distribution. These variations stem from differing proportions and contents of amylose and amylopectin during starch biosynthesis, as well as variances in the spatial folding and circling of different chain lengths and branches within the granules. Briant correlated lower mealiness with a high percentage of small starch granules¹⁸, while Barrios found larger granules in mealy Russet Burbank than in LaSoda¹⁹. However, starch granule size has not been found to be related to mealiness by other researchers²⁰.

Fig. 2.

Morphologies of starchgranules under scanning electron microscope(SEM) and the granule size distribution of starches.

Degree of branching, molecular weight and amylopectin chain length distribution of potato starches with different texture.

The data in Table 2 presents the degree of branching, weight-average molecular mass (Mw), and number average molecular mass (Mn) of potato starches with varying textures. It was observed that waxy potato starches (9.44%, 6.83% and 8.10%) exhibited a higher degree of branching compared to mealy potato starches (5.16%, 4.78% and 5.99%). Notably, amylose was linked by α−1,4-glycosidic bond, while amylopectin was primarily linked by α−1,6-glycosidic bond, with a minor linkage by α−1,4-glycosidic bond. Consequently, the degree of branching displayed a negative correlation with the amylose content. Starches with higher molecular weight predominantly consisted of amylopectin. The results indicated that both Mw (5955.06 kDa) and Mn (4019.37 kDa) were lower in mealy potato starches compared to waxy potato starches (6640.73 and 4688.50 kDa, respectively). Previous studies have reported that the Mw of amylopectin surpasses that of amylose²¹. Given the higher amylopectin content in waxy potato starches, they exhibited higher Mw and Mn. Additionally, studies by Yang have shown that waxy proso and foxtail millets also display higher Mw¹³. Similarly, research by Li has indicated that rice starch with high amylose content tends to have smaller amylopectin and amylose molecular sizes²², aligning with the findings in potatoes.

The distribution of amylopectin chain lengths significantly influences the functional properties of starch, including retrogradation, pasting, gelation, and digestion²³. A longer average chain length of amylopectin renders the starch structure more resistant to hydrolysis by amylase^24,25. The chain length distribution of amylopectin, as presented in Table 3; Fig. 3B, displays a bimodal distribution peaking at DP12. According to the clustering model described by Hizukuri{Hizukuri, 1986 #49}, amylopectin was categorized into A chain, B1 chain (DP13-24), B2 chain (DP25-36), and B3 chain (DP > 36), with A chain and B1 chain representing short chains, and B2 chain and B3 chain representing long chains. The average chain length of amylopectin in mealy potato starches (21.64%, 21.97% and 21.80%) was higher than that of waxy potato starches (21.15%, 21.06% and 21.24%). In comparison with mealy potato starches, waxy potato starches exhibited higher proportions of A chain and DP13-24, but lower amounts of DP25-37 and DP ≥ 37. Furthermore, waxy potato starches displayed a higher proportion of A chain, while mealy potato starches had higher proportions of DP25-37 and DP ≥ 37. These results indicate that waxy potato starches have a higher proportion of short chains and a lower proportion of long chains, consistent with studies in rice, maize, proso, and foxtail millets^13,26.

Table 3.

The amylopection chain length distribution, average chain length of amylopectin, relative crystallinity and IR ratio.

Sample ID	Average Chain Length of Amylopectin (%)	Relative Crystallinity (%)	Starches IR ratio	Chain Length Distribution (%)
			1047/1022cm −1	DP6−12	DP13−24	DP25−37	DP ≥ 37	(A + B1)/(B2 + B3)
M1	21.64abc	31.28c	0.90ab	19.22d	53.56a	15.89 a	11.34c	2.62b
M2	21.97a	38.00a	0.89b	20.54c	51.64b	14.45bc	13.37ab	2.76ab
M3	21.80ab	29.07c	0.89b	21.97b	50.42c	14.08c	13.54a	2.68ab
Mean	21.80	32.78	0.89	20.58	51.87	14.81	12.75	2.69
W1	21.15bc	27.38d	0.92a	22.78ab	50.65bc	15.06 b	11.51c	2.81ab
W2	21.06c	26.68dc	0.91ab	23.30a	51.26bc	13.39d	12.06abc	2.93a
W3	21.24bc	26.12dc	0.91ab	22.55ab	51.19bc	14.36c	11.89bc	2.60b
Mean	21.15	26.73	0.91	22.88	51.03	14.27	11.82	2.78

Values in the same column with different letters are significantly different (p<0.05).

Fig. 3.

(A) Ordered structure (FTIR) of starches (B)Amylopectin chain length distribution (C) X-ray diffraction(XRD) patterns.

Fourier transform infrared spectroscopyof potato starches with different texture

To examine the short-range molecular order of mealy and waxy potato starches, we acquired deconvoluted spectra within the 1200 –900 cm⁻¹ range (Fig. 3A). The FTIR bands at 1047 and 1022 cm⁻¹ are responsive to alterations in the crystalline and amorphous regions, respectively. The 1047/1022 cm⁻¹absorbance ratio serves as a commonly utilized indicator of short-range ordered structure in starch²⁷. The absorbance ratios at 1047/1022 cm⁻¹ for potato starches are outlined in Table 3. Notably, the waxy potato starches exhibited elevated absorbance ratios at 1047/1022 cm⁻¹(0.92, 0.91 and 0.91). This outcome suggests that waxy potato starches possess a greater amount of short-range ordered structure compared to mealy potato starches. This finding aligns with the results pertaining to the proportion of intermediate chains and DP 13–24 (Table 3), which primarily contribute to the formation of double helices.

Crystalline structure of potato starches with different texture

The X-ray diffraction patterns of various potato starches are depicted in Fig. 3C. These starches exhibited both sharp and diffuse diffraction characteristics, with distinct sharp diffraction peaks observed at 2θ of 5.6°, 15°, 17.2°, 22.2° and 24°, indicative of the typical “B” type crystal structure. The relative crystallinity of mealy potato starches, as determined from X-ray diffraction patterns, ranged from 29.07 to 38.00%, while that of waxy potato starches ranged from 26.12 to 27.38% (see Table 3). The discrepancy between the order of relative crystallinity and the short-range ordered structure determined by FTIR is notable. Amylopectin is attributed to the formation of crystallites in starch, with waxy starch exhibiting higher amylopectin content and thus higher crystallinity compared to mealy starch. Furthermore, waxy potato starches displayed a greater amount of short-range molecular order of double helices but slightly lower crystallinity compared to mealy potato starches, suggesting a lower amount of double helices in mealy starches are arranged into crystallites.

Pasting properties of potato starches with different texture

When an aqueous starch solution was heated, starch granules undergo water absorption and swelling. Upon reaching maximum swelling capacity, the granules rupture, releasing their amylose and amylopectin content. The critical aspect of pasting involves disrupting crystalline and double helical structures²⁸. Peak viscosity, trough viscosity, breakdown, final viscosity, and setback of mealy potato starches ranged from 2038 to 2535 cP, 1706–1796 cP, 242–777 cP, 2022–2180 cP and 297–385 cP, respectively. Waxy potato starches exhibited values of 2850–3226 cP, 1273–1453 cP, 1576–1813 cP, 1478–1713 cP and 204–260 cP, respectively. Notably, peak viscosity and breakdown were higher for waxy potato starches compared to mealy potato starches, while trough viscosity, final viscosity, and setback were higher for mealy potato starches compared to waxy potato starches. A study on proso millet revealed a strong negative correlation between amylose content and peak viscosity, trough viscosity, and breakdown. Additionally, the study reported a strong positive correlation between amylose content, setback, and pasting temperature¹³, consistent with our findings.

Table 4.

Pasting properties of mealy and waxy potato starches.

Sample ID	PeakViscosity/cP	Trough Viscosity/cP	Breakdown/cP	Final Viscosity/cP	Setback/cP	PeakTime/min	Pasting temperature/℃
M-1	2535c	1758b	777c	2055b	297b	5.49b	70.18b
M-2	2073d	1706c	367d	2022b	317b	5.54b	70.32b
M-3	2038d	1796a	242e	2180a	385a	6.01a	71.34a
Mean	2215.33	1753.33	462.00	2085.67	333.00	5.68	70.61
W-1	2850b	1273f	1576b	1478e	204d	3.78c	68.71c
W-2	3166a	1353e	1813a	1557d	204d	3.76c	68.57c
W-3	3226a	1453d	1773a	1713c	260c	3.90c	68.73c
Mean	3080.67	1359.67	1720.67	1582.67	222.67	3.81	68.67

Values in the same column with different letters are significantly different (p<0.05).

Correlation analysis of starch fine structure with texture

Correlation analyses were conducted (refer to Fig. 4) to further elucidate the relationship between starch fine structure and potato texture. The hardness of mealy potatoes was observed to be lower than that of waxy potatoes, with potato tuber hardness exhibiting significant positive correlations with PV, BD, DB, DP6-12, and 1047/1022 cm⁻¹, and significant negative correlations with TV, FV, SB, PT, PTM, AM and ACL. Amylose content emerged as the primary factor influencing the physicochemical properties of starch²⁹. Zhou et al. reported significant correlations between amylose content and various pasting parameters³⁰. Amylose content demonstrated positive correlations with Trough Viscosity, Final Viscosity, Setback, Peak Time, and Pasting Temperature, while exhibiting negative correlations with peak viscosity and breakdown³⁰. A study on rice showed that cooked rice from rice cultivars with high amylose content tends to be dry, fluffy, separate, and hard, while cooked rice with low amylose content tends to be glossy, soft, and sticky^31,32.

Fig. 4.

Correlation coefficients between structural and physicochemical properties of the starches. PV: Peak Viscosity; TV: Trough Viscosity; BD: Breakdown; FV: Final Viscosity; SB: Setback; PT: PeakTime; PTM: Pasting temperature; AM: Amylose Content; DB: Degree of Branching; Mw: Molecular Weight; RC: Relative Crystallinity; ACL: Average Chain Length. of Amylopectin; HD: Hardness.

Conclusions

The present investigation sought to elucidate the relationship between potato texture and starch fine structure through a comprehensive analysis of potato starch granules. Employing advanced physical techniques, this study meticulously examined the morphology, particle size distribution, crystalline architecture, molecular configuration, and granule heterogeneity of various potato starches. The findings underscored significant differences in starch granules associated with distinct textural attributes: mealy potatoes displayed smaller particle sizes relative to waxiness counterparts. Despite both categories manifesting the B-type crystalline pattern, variations in relative crystallinity were evident, with mealy potato starches demonstrating elevated levels. Moreover, mealy potato starches featured reduced amylopectin content, degree of branching, short-chain amylopectin fraction, and molecular weight compared to their waxiness counterparts. These alterations in starch molecular architecture were identified as pivotal factors governing the assembly of crystalline structures and shaping granule morphology, thereby influencing texture development. Collectively, these insights contribute to a deeper understanding of how subtle changes in starch fine structure can profoundly influence the textural characteristics and practical applications of potatoes, offering valuable guidance for breeders, food scientists, and industry stakeholders aiming to optimize potato quality and utility based on specific end-use requirements.

Author contributions

Conceptualization, Ying Wang; Methodology, Wenjing Huang, Fengkui Zeng, Jitian He and Zhechao Pan; Writing-Original Draft, Ying Wang; Supervision, and Darft Editing, Xianping Li, Zulfiqar Ali Sahito and Wanlin Yang. All authors have reviewed the manuscript.

Data availability

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Declarations

Competing interests

The authors declare no competing interests.

Ethics approval and consent to participate

Not applicable for that section.

Consent for publication

All authors agreed to publish this manuscript.