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. 1963 Feb;86(2):378–382. doi: 10.1042/bj0860378

Detection and resolution of gangliosides in lipid extracts by thin-layer chromatography

J R Wherrett 1,*, J N Cumings 1
PMCID: PMC1201764  PMID: 14000254

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOMSTRAND R., AHRENS E. H., Jr Absorption of fats studied in a patient with chyluria. III. Cholesterol. J Biol Chem. 1958 Aug;233(2):327–330. [PubMed] [Google Scholar]
  2. BOGOCH S. Studies on the structure of brain ganglioside. Biochem J. 1958 Feb;68(2):319–326. doi: 10.1042/bj0680319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOOTH D. A. The isolation and assay of gangliosides and their interactions with basic proteins. J Neurochem. 1962 May-Jun;9:265–276. doi: 10.1111/j.1471-4159.1962.tb09448.x. [DOI] [PubMed] [Google Scholar]
  4. CHATAGNON C., CHATAGNON P. Etude sur le strandin de Folch. I. Bull Soc Chim Biol (Paris) 1953;35(11-12):1319–1324. [PubMed] [Google Scholar]
  5. FOLCH-PI J., LEES M. Studies on the brain ganglioside strandin in normal brain and in Tay-Sachs' disease. AMA J Dis Child. 1959 May;97(5 Pt 2):730–738. doi: 10.1001/archpedi.1959.02070010732012. [DOI] [PubMed] [Google Scholar]
  6. FOLCH J., ARSOVE S., MEATH J. A. Isolation of brain strandin, a new type of large molecule tissue component. J Biol Chem. 1951 Aug;191(2):819–831. [PubMed] [Google Scholar]
  7. FOLCH J., ASCOLI I., LEES M., MEATH J. A., LeBARON N. Preparation of lipide extracts from brain tissue. J Biol Chem. 1951 Aug;191(2):833–841. [PubMed] [Google Scholar]
  8. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  9. JATZKEWITZ H., MEHL E. [On thin layer chromatography of cerebral lipids, their conversion and decomposition products]. Hoppe Seylers Z Physiol Chem. 1960 Oct 17;320:251–257. doi: 10.1515/bchm2.1960.320.1.251. [DOI] [PubMed] [Google Scholar]
  10. KLENK E., GIELEN W. [On a chromatographically standard, hexosamine-containing ganglioside from the human brain]. Hoppe Seylers Z Physiol Chem. 1961 Nov 24;326:158–161. doi: 10.1515/bchm2.1961.326.1.158. [DOI] [PubMed] [Google Scholar]
  11. KLENK E., GIELEN W. [On brain gangliosides]. Hoppe Seylers Z Physiol Chem. 1961 Mar 15;323:126–128. doi: 10.1515/bchm2.1961.323.1.126. [DOI] [PubMed] [Google Scholar]
  12. KLENK E., GIELEN W. [On studies on the brain gangliosides]. Hoppe Seylers Z Physiol Chem. 1960;319:283–286. doi: 10.1515/bchm2.1960.319.1.283. [DOI] [PubMed] [Google Scholar]
  13. KLENK E., GIELEN W. [Research on the constitution of a ganglioside from human brain and the separation of the mixture into the components]. Hoppe Seylers Z Physiol Chem. 1961 Nov 24;326:144–157. doi: 10.1515/bchm2.1961.326.1.144. [DOI] [PubMed] [Google Scholar]
  14. KLENK E. Zur Kenntnis der Ganglioside. Hoppe Seylers Z Physiol Chem. 1951 Nov;288(4-6):216–220. [PubMed] [Google Scholar]
  15. LONG C., STAPLES D. A. Determination of neuraminic acid in crude brain lipids. Biochem J. 1959 Nov;73:385–389. doi: 10.1042/bj0730385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PAYNE S. N., PLATT B. S. The quantitative separation and estimation of glycosphingosides in nervous tissue, with especial reference to gangliosides. J Neurochem. 1961 Apr;7:5–15. doi: 10.1111/j.1471-4159.1961.tb13491.x. [DOI] [PubMed] [Google Scholar]
  17. ROSENBERG A., CHARGAFF E. A study of a mucolipide from ox brain. J Biol Chem. 1958 Jun;232(2):1031–1049. [PubMed] [Google Scholar]
  18. STAHL E. Dünnschicht-Chromatographie; Methode, Einflussfaktoren und einige Anwendungsbeispiele. Pharmazie. 1956 Oct;11(10):633–637. [PubMed] [Google Scholar]
  19. SVENNERHOLM L. Composition of gangliosides from human brain. Nature. 1956 Mar 17;177(4507):524–525. doi: 10.1038/177524b0. [DOI] [PubMed] [Google Scholar]
  20. SVENNERHOLM L. Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957 Jun;24(3):604–611. doi: 10.1016/0006-3002(57)90254-8. [DOI] [PubMed] [Google Scholar]
  21. SVENNERHOLM L., RAAL A. Composition of brain ganglio-sides. Biochim Biophys Acta. 1961 Oct 28;53:422–424. doi: 10.1016/0006-3002(61)90460-7. [DOI] [PubMed] [Google Scholar]
  22. TRAMS E. G., LAUTER C. J. On the isolation and characterization of gangliosides. Biochim Biophys Acta. 1962 Jul 2;60:350–358. doi: 10.1016/0006-3002(62)90410-9. [DOI] [PubMed] [Google Scholar]
  23. VAN HEYNINGEN W. E., MILLER P. A. The fixation of tetanus toxin by ganglioside. J Gen Microbiol. 1961 Jan;24:107–119. doi: 10.1099/00221287-24-1-107. [DOI] [PubMed] [Google Scholar]
  24. WAGNER H., HOERHAMMER L., WOLFF P. [Thin layer chromatography of phosphatides and glycolipids]. Biochem Z. 1961;334:175–184. [PubMed] [Google Scholar]
  25. WHERRETT J. R., McILWAIN H. Gangliosides, phospholipids, protein and ribonucleic acid in subfractions of cerebral microsomal material. Biochem J. 1962 Aug;84:232–237. doi: 10.1042/bj0840232. [DOI] [PMC free article] [PubMed] [Google Scholar]

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