Abstract
Genic variation in natural populations of Drosophila simulans was surveyed using allozymic and two-dimensional electrophoretic techniques. Consistent with some previous reports, allozymic heterozygosity appeared lower than in the sibling species D. melanogaster (0.07 vs. 0.16). No variation was detected by two-dimensional electrophoresis of 19 lines scored for 70 abundant proteins. This is consistent with reported reductions in estimates of genic heterozygosity by two-dimensional electrophoresis in D. melanogaster, Mus musculus, and man. Although the amount of intraspecific variation detected in abundant proteins was lower than that detected for allozymes in D. simulans and D. melanogaster, the genetic distances between the sibling species calculated from the two data sets are not significantly different (0.35 and 0.20). The allozyme and two-dimensional electrophoresis data confirmed the impression from other measures of genetic variation (mitochondrial DNA restriction maps and inversion polymorphisms) that D. simulans is substantially less variable than D. melanogaster.
Full Text
The Full Text of this article is available as a PDF (821.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gillespie J. H. Molecular evolution and polymorphism in a random environment. Genetics. 1979 Nov;93(3):737–754. doi: 10.1093/genetics/93.3.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KIMURA M., CROW J. F. THE NUMBER OF ALLELES THAT CAN BE MAINTAINED IN A FINITE POPULATION. Genetics. 1964 Apr;49:725–738. doi: 10.1093/genetics/49.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kojima K., Gillespie J., Toari Y. N. A profile of Drosophila species' enzymes assayed by electrophoresis. I. Number of alleles, heterozygosities, and linkage disequilibrium in glucose-metabolizing systems and some other enzymes. Biochem Genet. 1970 Oct;4(5):627–637. doi: 10.1007/BF00486100. [DOI] [PubMed] [Google Scholar]
- Lewontin R. C. Population genetics. Annu Rev Genet. 1973;7:1–17. doi: 10.1146/annurev.ge.07.120173.000245. [DOI] [PubMed] [Google Scholar]
- McConkey E. H., Taylor B. J., Phan D. Human heterozygosity: a new estimate. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6500–6504. doi: 10.1073/pnas.76.12.6500. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 1978 Jul;89(3):583–590. doi: 10.1093/genetics/89.3.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ohnishi S., Voelker R. A. Comparative studies of allozyme loci in Drosophila simulans and Drosophila melanogaster. I. Three dipeptidase loci. Biochem Genet. 1981 Feb;19(1-2):75–85. doi: 10.1007/BF00486138. [DOI] [PubMed] [Google Scholar]
- Voelker R. A., Langley C. H., Brown A. J., Ohnishi S., Dickson B., Montgomery E., Smith S. C. Enzyme null alleles in natural populations of Drosophila melanogaster: Frequencies in a North Carolina population. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1091–1095. doi: 10.1073/pnas.77.2.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walton K. E., Styer D., Gruenstein E. I. Genetic polymorphism in normal human fibroblasts as analyzed by two-dimensional polyacrylamide gel electrophoresis. J Biol Chem. 1979 Aug 25;254(16):7951–7960. [PubMed] [Google Scholar]