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. 1982 Apr;100(4):597–614. doi: 10.1093/genetics/100.4.597

Indirect Suppression Involving Behavioral Mutants with Altered Nerve Excitability in DROSOPHILA MELANOGASTER

Barry Ganetzky 1,2, Chun-Fang Wu 1,2
PMCID: PMC1201835  PMID: 17246073

Abstract

Two classes of X-linked behavioral mutants of Drosophila melanogaster, leg-shaking mutants and bang-sensitive mutants, are suppressed by napts (no action potential, temperature-sensitive), an autosomal temperature-sensitive paralytic mutation. So far, napts is found to suppress thirteen mutations at seven loci, two of which produce leg shaking and five bang-sensitivity. Suppression is recessive, occurs at temperatures permissive for napts, and is indirect and function-specific rather than allele-specific. At restrictive temperatures, napts is known to completely block all nerve activity. Several of the mutants suppressed by napts are shown by neurophysiological experiments to have increased nerve excitability. The physiological defect of these mutants as well as their behavioral defect is suppressed by napts. Thus, suppression occurs within individual neurons at the level of excitable membranes and apparently depends on the reduction in membrane excitability caused by napts even under permissive conditions. We suggest that all mutants suppressed by napts may have related defects leading to enhanced nerve excitability. Genetic interactions of this type help reveal functional relationships between different behavioral mutants and suggest ways of isolating new mutants with altered excitable membranes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hall J. C., Greenspan R. J. Genetic analysis of Drosophila neurobiology. Annu Rev Genet. 1979;13:127–195. doi: 10.1146/annurev.ge.13.120179.001015. [DOI] [PubMed] [Google Scholar]
  2. Hartman P. E., Roth J. R. Mechanisms of suppression. Adv Genet. 1973;17:1–105. doi: 10.1016/s0065-2660(08)60170-4. [DOI] [PubMed] [Google Scholar]
  3. Hartshorne R. P., Catterall W. A. Purification of the saxitoxin receptor of the sodium channel from rat brain. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4620–4624. doi: 10.1073/pnas.78.7.4620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Homyk T., Jr, Szidonya J., Suzuki D. T. Behavioral mutants of Drosophila melanogaster. III. Isolation and mapping of mutations by direct visual observations of behavioral phenotypes. Mol Gen Genet. 1980;177(4):553–565. doi: 10.1007/BF00272663. [DOI] [PubMed] [Google Scholar]
  5. Homyk T., Sheppard D. E. Behavioral Mutants of DROSOPHILA MELANOGASTER. I. Isolation and Mapping of Mutations Which Decrease Flight Ability. Genetics. 1977 Sep;87(1):95–104. doi: 10.1093/genetics/87.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jan Y. N., Jan L. Y., Dennis M. J. Two mutations of synaptic transmission in Drosophila. Proc R Soc Lond B Biol Sci. 1977 Jul 28;198(1130):87–108. doi: 10.1098/rspb.1977.0087. [DOI] [PubMed] [Google Scholar]
  7. Jarvik J., Botstein D. Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2738–2742. doi: 10.1073/pnas.72.7.2738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Judd B. H., Shen M. W., Kaufman T. C. The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics. 1972 May;71(1):139–156. doi: 10.1093/genetics/71.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaplan W. D., Trout W. E., 3rd The behavior of four neurological mutants of Drosophila. Genetics. 1969 Feb;61(2):399–409. doi: 10.1093/genetics/61.2.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kung C., Chang S. Y., Satow Y., Houten J. V., Hansma H. Genetic dissection of behavior in paramecium. Science. 1975 May 30;188(4191):898–904. [PubMed] [Google Scholar]
  11. Riddle D. L., Brenner S. Indirect suppression in Caenorhabditis elegans. Genetics. 1978 Jun;89(2):299–314. doi: 10.1093/genetics/89.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tanouye M. A., Ferrus A., Fujita S. C. Abnormal action potentials associated with the Shaker complex locus of Drosophila. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6548–6552. doi: 10.1073/pnas.78.10.6548. [DOI] [PMC free article] [PubMed] [Google Scholar]

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