Skip to main content
NCBI home page
Genetics logoLink to Genetics
. 1982 Sep;102(1):91–99. doi: 10.1093/genetics/102.1.91

Molecular Characterization of Ribosomal Genes on the Ybb- Chromosome of DROSOPHILA MELANOGASTER

Sharyn A Endow 1
PMCID: PMC1201928  PMID: 6813192

Abstract

The question of whether the Ybb- chromosome contains ribosomal genes has been examined by using Southern blot analysis and comparing rDNA hybridization patterns for X/X and X/Ybb- DNA. The results demonstrate that the Ybb- chromosome contains sequences that hybridize to an rDNA probe under stringent conditions. Differential hybridization of some of these sequences with DNAs corresponding to different regions of a complete ribosomal gene repeat provides evidence that some of the genes on the Ybb- chromosome are type 2 repeats. Because data obtained by other workers suggest that type 2 repeats are transcribed only to low levels, these repeats may be classed as "nonfunctional". A further finding is that the ribosomal genes on the Ybb- chromosome do not undergo multiple rounds of DNA replication during polytenization of X/Ybb- cells.

Full Text

The Full Text of this article is available as a PDF (2.8 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Endow S. A., Glover D. M. Differential replication of ribosomal gene repeats in polytene nuclei of Drosophila. Cell. 1979 Jul;17(3):597–605. doi: 10.1016/0092-8674(79)90267-8. [DOI] [PubMed] [Google Scholar]
  2. Endow S. A. On ribosomal gene compensation in Drosophila. Cell. 1980 Nov;22(1 Pt 1):149–155. doi: 10.1016/0092-8674(80)90163-4. [DOI] [PubMed] [Google Scholar]
  3. Endow S. A. Polytenization of the ribosomal genes on the X and Y chromosomes of Drosophila melanogaster. Genetics. 1982 Mar;100(3):375–385. doi: 10.1093/genetics/100.3.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glover D. M., Hogness D. S. A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA. Cell. 1977 Feb;10(2):167–176. doi: 10.1016/0092-8674(77)90212-4. [DOI] [PubMed] [Google Scholar]
  5. Henderson A., Ritossa F. On the inheritance of rDNA of magnified bobbed loci in D. melanogaster. Genetics. 1970 Nov;66(3):463–473. doi: 10.1093/genetics/66.3.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kidd S. J., Glover D. M. Drosophila melanogaster ribosomal DNA containing type II insertions is variably transcribed in different strains and tissues. J Mol Biol. 1981 Oct 5;151(4):645–662. doi: 10.1016/0022-2836(81)90428-9. [DOI] [PubMed] [Google Scholar]
  7. Long E. O., Collins M., Kiefer B. I., Dawid I. B. Expression of the ribosomal DNA insertions in bobbed mutants of Drosophila melanogaster. Mol Gen Genet. 1981;182(3):377–384. doi: 10.1007/BF00293925. [DOI] [PubMed] [Google Scholar]
  8. Long E. O., Rebbert M. L., Dawid I. B. Structure and expression of ribosomal RNA genes of Drosophila melanogaster interrupted by type-2 insertions. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):667–672. doi: 10.1101/sqb.1981.045.01.084. [DOI] [PubMed] [Google Scholar]
  9. Ritossa F., Malva C., Boncinelli E., Graziani F., Polito L. The first steps of magnification of DNA complementary to ribosomal RNA in Drosophila malanogaster. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1580–1584. doi: 10.1073/pnas.68.7.1580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Spear B. B., Gall J. G. Independent control of ribosomal gene replication in polytene chromosomes of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1973 May;70(5):1359–1363. doi: 10.1073/pnas.70.5.1359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Spear B. B. The genes for ribosomal RNA in diploid and polytene chromosomes of Drosophila melanogaster. Chromosoma. 1974;48(2):159–179. doi: 10.1007/BF00283961. [DOI] [PubMed] [Google Scholar]
  12. Tartof K. D. Increasing the multiplicity of ribosomal RNA genes in Drosophila melanogaster. Science. 1971 Jan 22;171(3968):294–297. doi: 10.1126/science.171.3968.294. [DOI] [PubMed] [Google Scholar]
  13. Tartof K. D. Regulation of ribosomal RNA gene multiplicity in Drosophila melanogaster. Genetics. 1973 Jan;73(1):57–71. doi: 10.1093/genetics/73.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES