Abstract
Pancreatic amylase in the mouse displays considerable quantitative genetic variation. Agar gel electrophoresis reveals that homozygous animals have either one form of the enzyme, type A, or two forms, type AB. Only few animals have been found that contradict this statement, namely among Mus musculus castaneus from Thailand, which has a single-banded B type. Double-banded homozygous specimens of various origins have different relative proportions of the two isoenzymes. By measuring the A:B ratios in such animals, a number of distinct haplotypes or amylase complexes, determining ratios ranging from 61% A:39% B to 12% A:88% B, have been recognized. These complexes differ also with respect to the total amount of amylase produced. If the reference stock C3H/As is given the value 1, then other haplotypes have values ranging from 1.0 to 0.27. Nineteen amylase haplotypes have been established in congenic lines on a C3H/As background. Some of these lines contain at least four active pancreatic amylase structural genes and breeding experiments have demonstrated that the genetic elements regulating total amylase production and relative proportions of the isoenzymes are located within the amylase complex, cis-acting, and very closely linked to the structural genes.
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Selected References
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- Bloor J. H., Meisler M. H., Nielsen J. T. Genetic determination of amylase synthesis in the mouse. J Biol Chem. 1981 Jan 10;256(1):373–377. [PubMed] [Google Scholar]
- Hagenbüchle O., Bovey R., Young R. A. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland. Cell. 1980 Aug;21(1):179–187. doi: 10.1016/0092-8674(80)90125-7. [DOI] [PubMed] [Google Scholar]
- Hagenbüchle O., Tosi M., Schibler U., Bovey R., Wellauer P. K., Young R. A. Mouse liver and salivary gland alpha-amylase mRNAs differ only in 5' non-translated sequences. Nature. 1981 Feb 19;289(5799):643–646. doi: 10.1038/289643a0. [DOI] [PubMed] [Google Scholar]
- Hjorth J. P. Altered salivary amylase gene in the mouse strain BXD-16. Heredity (Edinb) 1982 Feb;48(Pt 1):127–135. doi: 10.1038/hdy.1982.13. [DOI] [PubMed] [Google Scholar]
- Hjorth J. P., Lusis A. J., Nielsen J. T. Multiple structural genes for mouse amylase. Biochem Genet. 1980 Apr;18(3-4):281–302. doi: 10.1007/BF00484242. [DOI] [PubMed] [Google Scholar]
- Hjorth J. P., Meisler M., Nielsen J. T. Genetic variation in amount of salivary amylase in the bank vole, Clethrionomys glareola. Genetics. 1979 Jul;92(3):915–930. doi: 10.1093/genetics/92.3.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MacDonald R. J., Crerar M. M., Swain W. F., Pictet R. L., Thomas G., Rutter W. J. Structure of a family of rat amylase genes. Nature. 1980 Sep 11;287(5778):117–122. doi: 10.1038/287117a0. [DOI] [PubMed] [Google Scholar]
- Nielsen J. T. Genetic studies of the amylase isoenzymes of the bank vole, Clethrionomys glareola. Hereditas. 1969;61(3):400–412. doi: 10.1111/j.1601-5223.1969.tb01852.x. [DOI] [PubMed] [Google Scholar]
- Owerbach D., Nielsen J. T., Rutter W. J. On the mechanism of variation of pancreatic amylase levels in mouse strains. J Biol Chem. 1981 Jun 25;256(12):6502–6506. [PubMed] [Google Scholar]
- Schibler U., Tosi M., Pittet A. C., Fabiani L., Wellauer P. K. Tissue-specific expression of mouse alpha-amylase genes. J Mol Biol. 1980 Sep 5;142(1):93–116. doi: 10.1016/0022-2836(80)90208-9. [DOI] [PubMed] [Google Scholar]
- Young R. A., Hagenbüchle O., Schibler U. A single mouse alpha-amylase gene specifies two different tissue-specific mRNAs. Cell. 1981 Feb;23(2):451–458. doi: 10.1016/0092-8674(81)90140-9. [DOI] [PubMed] [Google Scholar]