Abstract
Recessive lysine-independent revertants were isolated from a ψ+ haploid strain of the yeast Saccharomyces cerevisiae containing one of the leucine-inserting UAA suppressors, SUP29, and various UAA mutations including lys1-1. The majority of the revertants were found to have recessive suppressors in addition to the pre-existing SUP29 mutation. The recessive suppressors were able to suppress only a very limited number of UAA mutations, and none of the UAG mutations thus far examined. The recessive inefficient UAA suppressors were assigned to three complementation groups, sup111, sup112, and sup113. A high incidence of gene conversion was observed for an allele of sup111. An antisuppressor acting on sup111, but not detectably on SUP29, was inadvertently obtained during the course of the study. Interactions between SUP29, sup111 and the antisuppressor asu12 were studied.
Full Text
The Full Text of this article is available as a PDF (733.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brandriss M. C., Stewart J. W., Sherman F., Botstein D. Substitution of serine caused by a recessive lethal suppressor in yeast. J Mol Biol. 1976 Apr 15;102(3):467–476. doi: 10.1016/0022-2836(76)90328-4. [DOI] [PubMed] [Google Scholar]
- Cox B. S. A recessive lethal super-suppressor mutation in yeast and other psi phenomena. Heredity (Edinb) 1971 Apr;26(2):211–232. doi: 10.1038/hdy.1971.28. [DOI] [PubMed] [Google Scholar]
- Culbertson M. R., Charnas L., Johnson M. T., Fink G. R. Frameshifts and frameshift suppressors in Saccharomyces cerevisiae. Genetics. 1977 Aug;86(4):745–764. doi: 10.1093/genetics/86.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerlach W. L. Genetic properties of some amber-ochre supersuppressors in Saccharomyces cerevisiae. Mol Gen Genet. 1975;138(1):53–63. doi: 10.1007/BF00268827. [DOI] [PubMed] [Google Scholar]
- Gilmore R. A., Stewart J. W., Sherman F. Amino acid replacements resulting from super-suppression of nonsense mutants of iso-1-cytochrome c from yeast. J Mol Biol. 1971 Oct 14;61(1):157–173. doi: 10.1016/0022-2836(71)90213-0. [DOI] [PubMed] [Google Scholar]
- Hawthorne D. C. UGA mutations and UGA suppressors in yeast. Biochimie. 1976;58(1-2):179–182. doi: 10.1016/s0300-9084(76)80368-9. [DOI] [PubMed] [Google Scholar]
- Ono B. I., Stewart J. W., Sherman F. Serine insertion caused by the ribosomal suppressor SUP46 in yeast. J Mol Biol. 1981 Apr 15;147(3):373–379. doi: 10.1016/0022-2836(81)90489-7. [DOI] [PubMed] [Google Scholar]
- Ono B. I., Wills N., Stewart J. W., Gesteland R. F., Sherman F. Serine-inserting UAA suppression mediated by yeast tRNASer. J Mol Biol. 1981 Aug 15;150(3):361–373. doi: 10.1016/0022-2836(81)90552-0. [DOI] [PubMed] [Google Scholar]
- Smirnov V. N., Kreier V. G., Lizlova L. V., Andrianova V. M., Inge-Vechtomov S. G. Recessive super-suppression in yeast. Mol Gen Genet. 1974 Mar 14;129(2):105–121. doi: 10.1007/BF00268625. [DOI] [PubMed] [Google Scholar]
- Surguchov A. P., Beretetskaya Y. V., Fominykch E. S., Pospelova E. M., SmirnovVN, Ter-Avanesyan M. D., Inge-Vechtomov S. G. Recessive suppression in yeast Saccharomyces cerevisiae is mediated by a ribosomal mutation. FEBS Lett. 1980 Feb 25;111(1):175–178. doi: 10.1016/0014-5793(80)80786-1. [DOI] [PubMed] [Google Scholar]