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. 1983 Jan;103(1):75–92. doi: 10.1093/genetics/103.1.75

Location of the LSP-1 Genes in Drosophila Species by IN SITU Hybridization

Hugh W Brock 1, David B Roberts 1
PMCID: PMC1202026  PMID: 17246102

Abstract

The locations of the larval serum protein one (LSP-1) α, β and γ genes were determined in Drosophila melanogaster and in 14 other species of Drosophila by in situ hybridization to polytene chromosomes. The LSP-1 α gene mapped to bands 11B on the X chromosome, the LSP-1 β gene mapped to bands 21D-E on chromosome 2L, and the LSP-1 γ gene mapped to band 61A in all the melanogaster subgroup species. In eight other species, both the LSP-1 α and β genes mapped to one site on Muller's element E which corresponds to chromosome 3R of D. melanogaster. No hybridization of LSP-1 γ was detected in these eight species. Restriction enzyme digestion and analysis of genomic DNA by filter transfer hybridization confirmed the presence of LSP-1 α-like and β-like genes in seven of these species. These results are discussed with respect to conservation of the chromosomal elements in the genus Drosophila.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alonso C., Berendes H. D. The location of 5S (ribosomal) RNA genes in Drosophila hydei. Chromosoma. 1975 Aug 11;51(4):347–356. doi: 10.1007/BF00326321. [DOI] [PubMed] [Google Scholar]
  2. Brock H. W., Roberts D. B. Comparison of the larval serum proteins of Drosophila melanogaster using one and two-dimensional peptide mapping. Eur J Biochem. 1980 May;106(1):129–135. doi: 10.1111/j.1432-1033.1980.tb06003.x. [DOI] [PubMed] [Google Scholar]
  3. Cohen M., Jr Evolution of 5S ribosomal RNA genes in the chromosomes of the virilis group of Drosophila. Chromosoma. 1976 Jun 23;55(4):359–371. doi: 10.1007/BF00292831. [DOI] [PubMed] [Google Scholar]
  4. DOBZHANSKY T. The chromosomes of Drosophila willistoni. J Hered. 1950 Jun;41(6):156–158. doi: 10.1093/oxfordjournals.jhered.a106117. [DOI] [PubMed] [Google Scholar]
  5. Evgen'ev M. B., Kolchinski A., Levin A., Preobrazhenskaya A. L., Sarkisova E. Heat-shock DNA homology in distantly related species of Drosophila. Chromosoma. 1978 Sep 11;68(4):357–365. doi: 10.1007/BF00327170. [DOI] [PubMed] [Google Scholar]
  6. Hayashi S., Gillam I. C., Delaney A. D., Tener G. M. Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA. J Histochem Cytochem. 1978 Aug;26(8):677–679. doi: 10.1177/26.8.99471. [DOI] [PubMed] [Google Scholar]
  7. KRIVSHENKO J. D. The chromosomal polymorphism of Drosophila busckii in natural populations. Genetics. 1963 Sep;48:1239–1258. doi: 10.1093/genetics/48.9.1239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LE CALVEZ J. Carte cytologique des chromosomes géants des glandes salivaires de Drosophilia immigrans Sturt. Chromosoma. 1953;6(2):170–174. doi: 10.1007/BF01259938. [DOI] [PubMed] [Google Scholar]
  9. Lakovaara S., Saura A. Location of enzyme loci in chromosomes of Drosophila willistoni. Experientia. 1972 Mar 15;28(3):355–356. doi: 10.1007/BF01928737. [DOI] [PubMed] [Google Scholar]
  10. Langridge J., Langridge P., Bergquist P. L. Extraction of nucleic acids from agarose gels. Anal Biochem. 1980 Apr;103(2):264–271. doi: 10.1016/0003-2697(80)90266-3. [DOI] [PubMed] [Google Scholar]
  11. Pardue M. L., Gall J. G. Nucleic acid hybridization to the DNA of cytological preparations. Methods Cell Biol. 1975;10:1–16. doi: 10.1016/s0091-679x(08)60727-x. [DOI] [PubMed] [Google Scholar]
  12. Pierce D. A., Lucchesi J. C. Dosage compensation of X-linked heat-shock puffs in Drosophila pseudoobscura. Chromosoma. 1980;76(3):245–254. doi: 10.1007/BF00327265. [DOI] [PubMed] [Google Scholar]
  13. Roberts D. B., Evans-Roberts S. The genetic and cytogenetic localization of the three structural genes coding for the major protein of drosophila larval serum. Genetics. 1979 Nov;93(3):663–679. doi: 10.1093/genetics/93.3.663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  15. Strobel E., Dunsmuir P., Rubin G. M. Polymorphisms in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979 Jun;17(2):429–439. doi: 10.1016/0092-8674(79)90169-7. [DOI] [PubMed] [Google Scholar]
  16. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wimber D. E., Wimber D. R. Sites of the 5S ribosomal genes in Drosophila. I. The multiple clusters in the virilis group. Genetics. 1977 May;86(1):133–148. doi: 10.1093/genetics/86.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]

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