Abstract
The RP4 replicon was detected as covalently-closed circular DNA in Caulobacter crescentus strains into which it had been transferred from Escherichia coli. RP4-mediated transfer of ColE1-associated markers into C. crescentus occurred, but only as the result of transposon-mediated events. Both transposition of a ColE1-associated marker onto RP4 and cointegration of ColE1 with RP4 were observed. Chimeric plasmids containing both a ColE1 and an RP4 origin of replication were stably maintained in C. crescentus , but similar plasmids lacking the RP4 origin of replication were not stably maintained in C. crescentus. Thus we show that the ColE1 replicon cannot be maintained in C. crescentus unless it is covalently linked to another replicon, such as RK2, that can be maintained.
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Selected References
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- Bender R. A. Improved generalized transducing bacteriophage for Caulobacter crescentus. J Bacteriol. 1981 Nov;148(2):734–735. doi: 10.1128/jb.148.2.734-735.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ely B. Transfer of drug resistance factors to the dimorphic bacterium Caulobacter crescentus. Genetics. 1979 Mar;91(3):371–380. doi: 10.1093/genetics/91.3.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Figurski D. H., Helinski D. R. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648–1652. doi: 10.1073/pnas.76.4.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Figurski D. H., Meyer R. J., Helinski D. R. Suppression of Co1E1 replication properties by the Inc P-1 plasmid RK2 in hybrid plasmids constructed in vitro. J Mol Biol. 1979 Sep 25;133(3):295–318. doi: 10.1016/0022-2836(79)90395-4. [DOI] [PubMed] [Google Scholar]
- Friedman D. I., Yarmolinsky M. B. Prevention of the lethality of induced lambda prophage by an isogenic lambda plasmid. Virology. 1972 Nov;50(2):472–481. doi: 10.1016/0042-6822(72)90398-4. [DOI] [PubMed] [Google Scholar]
- Goldberg R. B., Bender R. A., Streicher S. L. Direct selection for P1-sensitive mutants of enteric bacteria. J Bacteriol. 1974 Jun;118(3):810–814. doi: 10.1128/jb.118.3.810-814.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hedges R. W., Jacob A. E. Transposition of ampicillin resistance from RP4 to other replicons. Mol Gen Genet. 1974;132(1):31–40. doi: 10.1007/BF00268228. [DOI] [PubMed] [Google Scholar]
- Heffron F., Kostriken R., Morita C., Parker R. Tn3 encodes a site-specific recombination system: identification of essential sequences, genes, and the actual site of recombination. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):259–268. doi: 10.1101/sqb.1981.045.01.038. [DOI] [PubMed] [Google Scholar]
- Johnson R. C., Ely B. Isolation of spontaneously derived mutants of Caulobacter crescentus. Genetics. 1977 May;86(1):25–32. doi: 10.1093/genetics/86.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POINDEXTER J. S. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP. Bacteriol Rev. 1964 Sep;28:231–295. doi: 10.1128/br.28.3.231-295.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothstein S. J., Reznikoff W. S. The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology. Cell. 1981 Jan;23(1):191–199. doi: 10.1016/0092-8674(81)90284-1. [DOI] [PubMed] [Google Scholar]
- Shapiro L. Differentiation in the Caulobacter cell cycle. Annu Rev Microbiol. 1976;30:377–407. doi: 10.1146/annurev.mi.30.100176.002113. [DOI] [PubMed] [Google Scholar]