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. 1983 May;104(1):1–9. doi: 10.1093/genetics/104.1.1

Involvement of Gene 49 in Recombination of Bacteriophage T4

Junichi Miyazaki 1, Yeikou Ryo 1, Teiichi Minagawa 1
PMCID: PMC1202056  PMID: 6345264

Abstract

The role of T4 gene 49 in recombination was investigated using its conditional-lethal amber (am) and temperature-sensitive (ts) mutants. When measured in genetic tests, defects in gene 49 produced a recombination-deficient phenotype. However, DNA synthesized in cells infected with a ts mutant (tsC9) at a nonpermissive temperature appeared to be in a recombinogenic state: after restitution of gene function by shifting to a permissive temperature, the recombinant frequency among progeny increased rapidly even when DNA replication was blocked by an inhibitor. Growth of a gene 49-defective mutant was suppressed by an additional mutation in gene uvsX, but recombination between rII markers was not.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernstein H. Repair and recombination in phage T4. I. Genes affecting recombination. Cold Spring Harb Symp Quant Biol. 1968;33:325–331. doi: 10.1101/sqb.1968.033.01.037. [DOI] [PubMed] [Google Scholar]
  2. Bernstein H. The effect on recombination of mutational defects in the DNA-polymerase and deoxycytidylate hydroxymethylase of phage T4D. Genetics. 1967 Aug;56(4):755–769. doi: 10.1093/genetics/56.4.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Black L. W., Silverman D. J. Model for DNA packaging into bacteriophage T4 heads. J Virol. 1978 Nov;28(2):643–655. doi: 10.1128/jvi.28.2.643-655.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Broker T. R. An electron microscopic analysis of pathways for bacteriophage T4 DNA recombination. J Mol Biol. 1973 Nov 25;81(1):1–16. doi: 10.1016/0022-2836(73)90243-x. [DOI] [PubMed] [Google Scholar]
  5. Broker T. R., Doermann A. H. Molecular and genetic recombination of bacteriophage T4. Annu Rev Genet. 1975;9:213–244. doi: 10.1146/annurev.ge.09.120175.001241. [DOI] [PubMed] [Google Scholar]
  6. Cunningham R. P., Berger H. Mutations affecting genetic recombination in bacteriophage T4D. I. Pathway analysis. Virology. 1977 Jul 1;80(1):67–82. doi: 10.1016/0042-6822(77)90381-6. [DOI] [PubMed] [Google Scholar]
  7. Dewey M. J., Frankel F. R. Two suppressor loci for gene 49 mutations of bacteriophage T4. I. Genetic properties and DNA synthesis. Virology. 1975 Dec;68(2):387–401. doi: 10.1016/0042-6822(75)90281-0. [DOI] [PubMed] [Google Scholar]
  8. Frankel F. R., Batcheler M. L., Clark C. K. The role of gene 49 in DNA replication and head morphogenesis in bacteriophage T4. J Mol Biol. 1971 Dec 28;62(3):439–463. doi: 10.1016/0022-2836(71)90147-1. [DOI] [PubMed] [Google Scholar]
  9. Fujisawa H., Minagawa T. Genetic control of the DNA maturation in the process of phage morphogenesis. Virology. 1971 Jul;45(1):280–291. [PubMed] [Google Scholar]
  10. Granboulan P., S echaud J., Kellenberger E. On the fragility of phage T4-related particles. Virology. 1971 Nov;46(2):407–425. doi: 10.1016/0042-6822(71)90042-0. [DOI] [PubMed] [Google Scholar]
  11. Hosoda J., Mathews E., Jansen B. Role of genes 46 and 47 in bacteriophage T4 reproduction. I. In vivo deoxyribonucleic acid replication. J Virol. 1971 Oct;8(4):372–387. doi: 10.1128/jvi.8.4.372-387.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kemper B., Brown D. T. Function of gene 49 of bacteriophage T4. II. Analysis of intracellular development and the structure of very fast-sedimenting DNA. J Virol. 1976 Jun;18(3):1000–1015. doi: 10.1128/jvi.18.3.1000-1015.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kemper B., Garabett M. Studies on T4-head maturation. 1. Purification and characterization of gene-49-controlled endonuclease. Eur J Biochem. 1981 Mar 16;115(1):123–131. [PubMed] [Google Scholar]
  14. King J. Assembly of the tail of bacteriophage T4. J Mol Biol. 1968 Mar 14;32(2):231–262. doi: 10.1016/0022-2836(68)90007-7. [DOI] [PubMed] [Google Scholar]
  15. Minagawa T., Ryo Y. Genetic control of formation of very fast sedimenting DNA of bacteriophage T4. Mol Gen Genet. 1979 Feb 16;170(1):113–115. doi: 10.1007/BF00268587. [DOI] [PubMed] [Google Scholar]
  16. Minagawa T., Ryo Y. Substrate specificity of gene 49-controlled deoxyribonuclease of bacteriophage T4: special reference to DNA packaging. Virology. 1978 Dec;91(2):222–233. doi: 10.1016/0042-6822(78)90371-9. [DOI] [PubMed] [Google Scholar]
  17. Mizuuchi K., Kemper B., Hays J., Weisberg R. A. T4 endonuclease VII cleaves holliday structures. Cell. 1982 Jun;29(2):357–365. doi: 10.1016/0092-8674(82)90152-0. [DOI] [PubMed] [Google Scholar]
  18. Nishimoto H., Takayama M., Minagawa T. Purification and some properties of deoxyribonuclease whose synthesis is controlled by gene 49 of bacteriophage T4. Eur J Biochem. 1979 Oct 15;100(2):433–440. doi: 10.1111/j.1432-1033.1979.tb04186.x. [DOI] [PubMed] [Google Scholar]
  19. Shah D. B., Berger H. Replication of gene 46-47 amber mutants of bacteriophage T4D. J Mol Biol. 1971 Apr 14;57(1):17–34. doi: 10.1016/0022-2836(71)90117-3. [DOI] [PubMed] [Google Scholar]
  20. Shah D. B., DeLorenzo L. Suppression of gene 49 mutations of bacteriophage T4 by a second mutation in gene X: structure of pseudorevertant DNA. J Virol. 1977 Dec;24(3):794–804. doi: 10.1128/jvi.24.3.794-804.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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