Abstract
Several aspects of the recombination resulting from λ plac5 transduction were investigated in strains of Escherichia coli K-12 that use the RecE or RecF recombination pathways. In a RecBC pathway strain, F42lac recombination with λplac5 is 20- to 50-fold higher than chromosomal lac times λplac5 recombination, and this recombination enhancement is largely dependent on constitutive expression of F42lac fertility functions. Here, it was observed that F42 lac fertility functions do not effect the ability of F42lac to recombine with λplac5 in a RecE or RecF pathway strain. Therefore, the enhancement observed in a Rec+ (or RecBC pathway) strain is directly dependent on the recBC gene product. The end product of recombination between λplac5 and either F42lac or chromosomal lac in RecE and RecF pathway strains was monitored by scoring for addition and substitution transductants. It was observed that the percentage of addition transductants was lower in all cases for RecE and RecF pathway strains as compared with RecBC pathway or a recB strain. It is concluded that the introduction of sbcA or sbcB into a recB strain produces a change in recombination mechanism that is reflected in the nature of the end product of recombination.
Full Text
The Full Text of this article is available as a PDF (868.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Birge E. A., Low K. B. Detection of transcribable recombination products following conjugation in rec+, reCB- and recC-strains of Escherichia coli K12. J Mol Biol. 1974 Mar 15;83(4):447–457. doi: 10.1016/0022-2836(74)90506-3. [DOI] [PubMed] [Google Scholar]
- Capaldo F. N., Ramsey G., Barbour S. D. Analysis of the growth of recombination-deficient strains of Escherichia coli K-12. J Bacteriol. 1974 Apr;118(1):242–249. doi: 10.1128/jb.118.1.242-249.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gillen J. R., Willis D. K., Clark A. J. Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12. J Bacteriol. 1981 Jan;145(1):521–532. doi: 10.1128/jb.145.1.521-532.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horii Z., Clark A. J. Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants. J Mol Biol. 1973 Oct 25;80(2):327–344. doi: 10.1016/0022-2836(73)90176-9. [DOI] [PubMed] [Google Scholar]
- Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter R. D. Enhanced recombination between F42lac and lambda plac5: dependence on F42lac fertility functions. Mol Gen Genet. 1981;184(3):355–358. doi: 10.1007/BF00352504. [DOI] [PubMed] [Google Scholar]
- Porter R. D., Lark M. W., Low K. B. Specialized transduction with lambda plac5: dependence on recA and on configuration of lac and att lambda. J Virol. 1981 May;38(2):497–503. doi: 10.1128/jvi.38.2.497-503.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter R. D., McLaughlin T., Low B. Transduction versus "conjuduction": evidence for multiple roles for exonuclease V in genetic recombination in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1043–1047. doi: 10.1101/sqb.1979.043.01.113. [DOI] [PubMed] [Google Scholar]
- Porter R. D. Recombination properties of P1 dlac. J Bacteriol. 1982 Oct;152(1):345–350. doi: 10.1128/jb.152.1.345-350.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter R. D., Welliver R. A., Witkowski T. A. Specialized transduction with lambda plac5: dependence on recB. J Bacteriol. 1982 Jun;150(3):1485–1488. doi: 10.1128/jb.150.3.1485-1488.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]