Skip to main content
Genetics logoLink to Genetics
. 1983 Nov;105(3):733–743. doi: 10.1093/genetics/105.3.733

Exceptionally High Levels of Restriction Site Polymorphism in DNA near the Maize Adh1 Gene

Mitrick A Johns 1, Judith N Strommer 1, Michael Freeling 1
PMCID: PMC1202183  PMID: 17246173

Abstract

Restriction maps have been prepared for the chromosomal region near seven biochemically and genetically distinct maize alcohol dehydrogenase-1 (Adh1) alleles using a small cDNA probe for Adh1. Five restriction sites spanning about 4 kb in and near the Adh1 transcription unit appear identical in all seven alleles. Outside this conserved region, variation in restriction site position is the rule. Six of the seven alleles are distinguishable, and the alleles appear to fall into four groups. The DNA flanking the 1S-type alleles seems to share no restriction site homology with the DNA near the 1F-type alleles. Several hypotheses are put forward to explain how such high levels of polymorphism could have arisen in a species that has been domesticated for only about 10,000 years.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Freeling M. Allelic variation at the level of intragenic recombination. Genetics. 1978 May;89(1):211–224. doi: 10.1093/genetics/89.1.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gerlach W. L., Pryor A. J., Dennis E. S., Ferl R. J., Sachs M. M., Peacock W. J. cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize. Proc Natl Acad Sci U S A. 1982 May;79(9):2981–2985. doi: 10.1073/pnas.79.9.2981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  4. Schwartz D., Laughner W. J. A molecular basis for heterosis. Science. 1969 Oct 31;166(3905):626–627. doi: 10.1126/science.166.3905.626. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES