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. 1984 Jan;106(1):1–16. doi: 10.1093/genetics/106.1.1

Cloning and Physical Mapping of an Early Region of the Bacteriophage T4 Genome

Paul M Macdonald 1, Gisela Mosig 1
PMCID: PMC1202239  PMID: 6319228

Abstract

We have cloned DNA restriction fragments from the largely nonessential region of bacteriophage T4 located between genes 39 and 56. The cloned DNA fragments were used to construct a precise map of the sites in this region recognized by eight restriction endonucleases. This restriction map allowed us to compare the cytosine-containing T4 DNA used for cloning with the hydroxymethylcytosine-containing DNA of wild-type T4; there were no detectable rearrangements in the region tested. We were also able to determine the physical locations of several deletion end points and of several genes.

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Selected References

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  1. Clewell D. B., Helinski D. R. Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol. 1972 Jun;110(3):1135–1146. doi: 10.1128/jb.110.3.1135-1146.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cupido M., Grimbergen J., De Groot B. Participation of bacteriophage T4 gene 41 in replication repair. Mutat Res. 1980 Apr;70(2):131–138. doi: 10.1016/0027-5107(80)90152-9. [DOI] [PubMed] [Google Scholar]
  3. Depew R. E., Cozzarelli N. R. Genetics and physiology of bacteriophage T4 3'-phosphatase: evidence for involvement of the enzyme in T4 DNA metabolism. J Virol. 1974 Apr;13(4):888–897. doi: 10.1128/jvi.13.4.888-897.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Emrich J. Lysis of T4-infected bacteria in the absence of lysozyme. Virology. 1968 May;35(1):158–165. doi: 10.1016/0042-6822(68)90315-2. [DOI] [PubMed] [Google Scholar]
  5. Fukada K., Gossens L., Abelson J. The cloning of a T4 transfer RNA gene cluster. J Mol Biol. 1980 Feb 25;137(2):213–234. doi: 10.1016/0022-2836(80)90326-5. [DOI] [PubMed] [Google Scholar]
  6. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guthrie C., McClain W. H. Conditionally lethal mutants of bacteriophage T4 defective in production of a transfer RNA. J Mol Biol. 1973 Dec 5;81(2):137–155. doi: 10.1016/0022-2836(73)90185-x. [DOI] [PubMed] [Google Scholar]
  8. Homyk T., Jr, Weil J. Deletion analysis of two nonessential regions of the T4 genome. Virology. 1974 Oct;61(2):505–523. doi: 10.1016/0042-6822(74)90286-4. [DOI] [PubMed] [Google Scholar]
  9. Horvitz H. R. Bacteriophage T4 mutants deficient in alteration and modification of the Escherichia coli RNA polymerase. J Mol Biol. 1974 Dec 25;90(4):739–750. doi: 10.1016/0022-2836(74)90537-3. [DOI] [PubMed] [Google Scholar]
  10. Ishii T., Yanagida M. Molecular organization of the shell of the Teven bacteriophage head. J Mol Biol. 1975 Oct 5;97(4):655–660. doi: 10.1016/s0022-2836(75)80065-9. [DOI] [PubMed] [Google Scholar]
  11. Kuhn B., Abdel-Monem M., Krell H., Hoffmann-Berling H. Evidence for two mechanisms for DNA unwinding catalyzed by DNA helicases. J Biol Chem. 1979 Nov 25;254(22):11343–11350. [PubMed] [Google Scholar]
  12. Mattson T., Van Houwe G., Bolle A., Selzer G., Epstein R. Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes. Mol Gen Genet. 1977 Sep 9;154(3):319–326. doi: 10.1007/BF00571289. [DOI] [PubMed] [Google Scholar]
  13. McClain W. H., Guthrie C., Barrell B. G. The psu1+ amber suppressor gene of bacteriophage T4: identification of its amino acid and transfer RNA. J Mol Biol. 1973 Dec 5;81(2):157–171. doi: 10.1016/0022-2836(73)90186-1. [DOI] [PubMed] [Google Scholar]
  14. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  15. Mileham A. J., Revel H. R., Murray N. E. Molecular cloning of the T4 genome; organization and expression of the frd--DNA ligase region. Mol Gen Genet. 1980;179(2):227–239. doi: 10.1007/BF00425449. [DOI] [PubMed] [Google Scholar]
  16. Neal M. W., Florini J. R. A rapid method for desalting small volumes of solution. Anal Biochem. 1973 Sep;55(1):328–330. doi: 10.1016/0003-2697(73)90325-4. [DOI] [PubMed] [Google Scholar]
  17. O'Farrell P. H., Kutter E., Nakanishi M. A restriction map of the bacteriophage T4 genome. Mol Gen Genet. 1980;179(2):421–435. doi: 10.1007/BF00425473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Revel H. R. Molecular cloning of the T4 genome: organization and expression of the tail fiber gene cluster 34--38. Mol Gen Genet. 1981;182(3):445–455. doi: 10.1007/BF00293934. [DOI] [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]

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