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. 1984 Apr;106(4):577–589. doi: 10.1093/genetics/106.4.577

Semidominance of rad18-2 for Several Phenotypic Characters in SACCHAROMYCES CEREVISIAE

Vernon W Mayer 1, Carol J Goin 1
PMCID: PMC1202290  PMID: 6370788

Abstract

Inbred diploid yeast strains heterozygous or homozygous for the rad18-2 allele and carrying markers for detection of mitotic recombination were constructed. The homozygous rad18-2/rad18-2 strain was highly sensitive to killing by UV light, showed greatly elevated frequencies of spontaneous and induced mitotic recombination and was more sensitive to trimethoprim than the wild-type diploid. The heterozygous strain RAD18/rad18-2 was intermediate in its response for these same phenotypic characters. These findings are discussed in the light of other studies in which incomplete dominance of genes involved in some aspect of DNA repair has been reported.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auerbach A. D., Wolman S. R. Carcinogen-induced chromosome breakage in Fanconi's anaemia heterozygous cells. Nature. 1978 Jan 5;271(5640):69–71. doi: 10.1038/271069a0. [DOI] [PubMed] [Google Scholar]
  2. Boram W. R., Roman H. Recombination in Saccharomyces cerevisiae: a DNA repair mutation associated with elevated mitotic gene conversion. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2828–2832. doi: 10.1073/pnas.73.8.2828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen P. C., Lavin M. F., Kidson C., Moss D. Identification of ataxia telangiectasia heterozygotes, a cancer prone population. Nature. 1978 Aug 3;274(5670):484–486. doi: 10.1038/274484a0. [DOI] [PubMed] [Google Scholar]
  4. Cox B. S., Parry J. M. The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast. Mutat Res. 1968 Jul-Aug;6(1):37–55. doi: 10.1016/0027-5107(68)90101-2. [DOI] [PubMed] [Google Scholar]
  5. Cox B., Game J. Repair systems in Saccharomyces. Mutat Res. 1974 Aug;26(4):257–264. doi: 10.1016/s0027-5107(74)80023-0. [DOI] [PubMed] [Google Scholar]
  6. Der Kaloustian V. M., de Weerd-Kastelein EA+DEWEERDAAKATELEIN E. A., Kleijer W. J., Keijzer W., Bootsma D. The genetic defect in the de Sanctis-Cacchione syndrome. J Invest Dermatol. 1974 Nov;63(5):392–396. doi: 10.1111/1523-1747.ep12676556. [DOI] [PubMed] [Google Scholar]
  7. GARRIGA S., CROSBY W. H. The incidence of leukemia in families of patients with hypoplasia of the marrow. Blood. 1959 Sep;14:1008–1014. [PubMed] [Google Scholar]
  8. Game J. C., Little J. G., Haynes R. H. Yeast mutants sensitive to trimethoprim. Mutat Res. 1975 May;28(2):175–182. doi: 10.1016/0027-5107(75)90094-9. [DOI] [PubMed] [Google Scholar]
  9. Haładus E., Zuk J. UV-induced mitotic recombination and its dependence on photoreactivation and liquid holding in the rad6-1 mutant of Saccharomyces cerevisiae. Mol Gen Genet. 1980;180(3):591–596. doi: 10.1007/BF00268065. [DOI] [PubMed] [Google Scholar]
  10. Henriques J. A., Moustacchi E. Isolation and characterization of pso mutants sensitive to photo-addition of psoralen derivatives in Saccharomyces cerevisiae. Genetics. 1980 Jun;95(2):273–288. doi: 10.1093/genetics/95.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ho K. S., Mortimer R. K. Induction of dominant lethality by x-rays in radiosensitive strain of yeast. Mutat Res. 1973 Oct;20(1):45–51. doi: 10.1016/0027-5107(73)90096-1. [DOI] [PubMed] [Google Scholar]
  12. Kern R., Zimmermann F. K. The influence of defects in excision and error prone repair on spontaneous and induced mitotic recombination and mutation in Saccharomyces cerevisiae. Mol Gen Genet. 1978 Apr 25;161(1):81–88. doi: 10.1007/BF00266618. [DOI] [PubMed] [Google Scholar]
  13. Kleijer W. J., de Weerd-Kastelein E. A., Sluyter M. L., Keijzer W., de Wit J., Bootsma D. UV-induced DNA repair synthesis in cells of patients with different forms of xeroderma pigmentosum and of heterozygotes. Mutat Res. 1973 Dec;20(3):417–428. doi: 10.1016/0027-5107(73)90062-6. [DOI] [PubMed] [Google Scholar]
  14. Maloney D. H., Fogel S. Mitotic recombination in yeast: isolation and characterization of mutants with enhanced spontaneous mitotic gene conversion rates. Genetics. 1980 Apr;94(4):825–839. doi: 10.1093/genetics/94.4.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mayer V. W., Hybner C. J., Brusick D. J. Genetic effects induced in Saccharomyces cerevisiae by cyclophosphamide in vitro without liver enzyme preparations. Mutat Res. 1976 Nov;37(2-3):201–212. doi: 10.1016/0027-5107(76)90034-8. [DOI] [PubMed] [Google Scholar]
  16. McKee R. H., Lawrence C. W. Genetic analysis of gamma-ray mutagenesis in yeast. II. Allele-specific control of mutagenesis. Genetics. 1979 Oct;93(2):375–381. doi: 10.1093/genetics/93.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McKee R. H., Lawrence C. W. Genetic analysis of gamma-ray mutagenesis in yeast. III. Double-mutant strains. Mutat Res. 1980 Mar;70(1):37–48. doi: 10.1016/0027-5107(80)90056-1. [DOI] [PubMed] [Google Scholar]
  18. Nakai S., Matsumoto S. Two types of radiation-sensitive mutant in yeast. Mutat Res. 1967 Mar-Apr;4(2):129–136. doi: 10.1016/0027-5107(67)90064-4. [DOI] [PubMed] [Google Scholar]
  19. Parry J. M., Cox B. S. The effects of dark holding and photoreactivation on ultraviolet light-induced mitotic recombination and survival in yeast. Genet Res. 1968 Oct;12(2):187–198. doi: 10.1017/s0016672300011794. [DOI] [PubMed] [Google Scholar]
  20. Paterson M. C., Anderson A. K., Smith B. P., Smith P. J. Enhanced radiosensitivity of cultured fibroblasts from ataxia telangiectasia heterozygotes manifested by defective colony-forming ability and reduced DNA repair replication after hypoxic gamma-irradiation. Cancer Res. 1979 Sep;39(9):3725–3734. [PubMed] [Google Scholar]
  21. Rodarte-Ramón U. S., Mortimer R. K. Radiation-induced recombination in Saccharomyces: isolation and genetic study of recombination-deficient mutants. Radiat Res. 1972 Jan;49(1):133–147. [PubMed] [Google Scholar]
  22. Zimmermann F. K., Gundelach E. Intragenic complementation, hybrid enzyme formation and dominance in diploid cells of Saccharomyces cerevisiae. Mol Gen Genet. 1969;103(4):348–362. doi: 10.1007/BF00383485. [DOI] [PubMed] [Google Scholar]
  23. Zimmermann F. K., Schmiedt I., ten Berge A. M. Dominance and recessiveness at the protein level in mutant x wildtype crosses in Sacchaomyces cerevisiae. Mol Gen Genet. 1969 Aug 15;104(4):321–330. doi: 10.1007/BF00334231. [DOI] [PubMed] [Google Scholar]

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