Abstract
Spontaneous mutation rates were estimated by assaying 84,126 seedlings of a highly homozygous barley line (isogenic line 2025) for five enzyme loci. No mutants were observed in 841,260 allele replications. This result excludes, at probability level 0.95, a spontaneous mutation rate larger than 3.56 x 10-6/locus/gamete/generation for these enzyme loci. Isogenic line 2025 also was scored for mutants at four loci governing morphological variants. No mutants were observed in 3,386,850 allele replications which indicates that the upper bound for the mutation rate for these loci is 8.85 x 10-7. It was concluded that, even though spontaneous mutation has been important in creating variability in the barley species at the loci scored, the rate is too low to have much affect on the short-term dynamics of barley populations.
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Selected References
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- Langley C. H., Voelker R. A., Brown A. J., Ohnishi S., Dickson B., Montgomery E. Null allele frequencies at allozyme loci in natural populations of Drosophila melanogaster. Genetics. 1981 Sep;99(1):151–156. doi: 10.1093/genetics/99.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mukai T., Cockerham C. C. Spontaneous mutation rates at enzyme loci in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2514–2517. doi: 10.1073/pnas.74.6.2514. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neel J. V., Mohrenweiser H. W., Meisler M. H. Rate of spontaneous mutation at human loci encoding protein structure. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6037–6041. doi: 10.1073/pnas.77.10.6037. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tobari Y. N., Kojima K. I. A study of spontaneous mutation rates at ten loci detectable by starch gel electrophoresis in Drosophila melanogaster. Genetics. 1972 Mar;70(3):397–403. doi: 10.1093/genetics/70.3.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Voelker R. A., Schaffer H. E., Mukai T. Spontaneous Allozyme Mutations in DROSOPHILA MELANOGASTER: Rate of Occurrence and Nature of the Mutants. Genetics. 1980 Apr;94(4):961–968. doi: 10.1093/genetics/94.4.961. [DOI] [PMC free article] [PubMed] [Google Scholar]