Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1984 Aug;107(4):563–576. doi: 10.1093/genetics/107.4.563

Genetics of CHLAMYDOMONAS REINHARDTII Diploids. II. the Effects of Diploidy and Aneuploidy on the Transmission of Non-Mendelian Markers

Eva M Eves 1,2, Kwen-Sheng Chiang 1,2
PMCID: PMC1202377  PMID: 6589201

Abstract

The transmission of two non-Mendelian drug resistance markers has been studied in crosses of Chlamydomonas reinhardtii involving diploids and aneuploids with different mating type genotypes. Under normal laboratory conditions for gametogenesis, mating and zygote maturation, the transmission pattern of the non-Mendelian markers sr-u-1 (resistance to streptomycin) and spr-u-1-27-3 (resistance to spectinomycin) is primarily determined by the mating type genotypes of the parental cells. Our results confirm and expand an earlier observation suggesting that an apparent codominant function of the female (mt+) allele in regulating chloroplast gene transmission in meiosis appears to be distinct and separate from its recessive function in regulating mating behavior. The chloroplast DNA complement (as indexed by the number of extranuclear DNA-containing bodies) may exert a secondary effect on the transmission of these markers. Within a mating type group (mt+/mt- or mt-/mt-) a cell line with more chloroplast DNA tended to transmit its non-Mendelian markers more frequently than a cell line with less chloroplast DNA.

Full Text

The Full Text of this article is available as a PDF (1.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birky C. W., Jr Transmission genetics of mitochondria and chloroplasts. Annu Rev Genet. 1978;12:471–512. doi: 10.1146/annurev.ge.12.120178.002351. [DOI] [PubMed] [Google Scholar]
  2. Bolen P. L., Grant D. M., Swinton D., Boynton J. E., Gillham N. W. Extensive methylation of chloroplast DNA by a nuclear gene mutation does not affect chloroplast gene transmission in chlamydomonas. Cell. 1982 Feb;28(2):335–343. doi: 10.1016/0092-8674(82)90351-8. [DOI] [PubMed] [Google Scholar]
  3. Chiang K. S., Friedman E., Malavasic M. J., Jr, Feng M. L., Eves E. M., Feng T. Y., Swinton D. C. On the folding and organization of chloroplast DNA in Chlamydomonas reinhardtii. Ann N Y Acad Sci. 1981;361:219–247. doi: 10.1111/j.1749-6632.1981.tb46521.x. [DOI] [PubMed] [Google Scholar]
  4. Coleman A. W. Visualization of chloroplast DNA with two fluorochromes. Exp Cell Res. 1978 Jun;114(1):95–100. doi: 10.1016/0014-4827(78)90040-x. [DOI] [PubMed] [Google Scholar]
  5. GILLHAM N. W. TRANSMISSION AND SEGREGATION OF A NON-CHROMOSOMAL FACTOR CONTROLLING STREPTOMYCIN RESISTANCE IN DIPLOID CHLAMYDOMONAS. Nature. 1963 Oct 19;200:294–294. doi: 10.1038/200294a0. [DOI] [PubMed] [Google Scholar]
  6. Gillham N. W., Boynton J. E., Lee R. W. Segregation and recombination of non-Mendellan genes in Chlamydomonas. Genetics. 1974 Sep;78(1):439–457. doi: 10.1093/genetics/78.1.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grant D. M., Gillham N. W., Boynton J. E. Inheritance of chloroplast DNA in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6067–6071. doi: 10.1073/pnas.77.10.6067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kuroiwa T., Kawano S., Nishibayashi S., Sato C. Epifluorescent microscopic evidence for maternal inheritance of chloroplast DNA. Nature. 1982 Jul 29;298(5873):481–483. doi: 10.1038/298481a0. [DOI] [PubMed] [Google Scholar]
  9. Matagne R. F., Hermesse M. P. Modification of Chloroplast Gene Transmission in Somatic Fusion Products and Vegetative Zygotes of CHLAMYDOMONAS REINHARDI by 5-Fluorodeoxyuridine. Genetics. 1981 Nov;99(3-4):371–381. doi: 10.1093/genetics/99.3-4.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matagne R. F., Mathieu D. Transmission of chloroplast genes in triploid and tetraploid zygospores of Chlamydomonas reinhardtii: Roles of mating-type gene dosage and gametic chloroplast DNA content. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4780–4783. doi: 10.1073/pnas.80.15.4780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RIS H., PLAUT W. Ultrastructure of DNA-containing areas in the chloroplast of Chlamydomonas. J Cell Biol. 1962 Jun;13:383–391. doi: 10.1083/jcb.13.3.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Royer H. D., Sager R. Methylation of chloroplast DNAs in the life cycle of Chlamydomonas. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5794–5798. doi: 10.1073/pnas.76.11.5794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sager R., Ramanis Z. Chloroplast genetics of chlamydomonas. I. Allelic segregation ratios. Genetics. 1976 Jun;83(2):303–321. doi: 10.1093/genetics/83.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sueoka N., Chiang K. S., Kates J. R. Deoxyribonucleic acid replication in meiosis of Chlamydomonas reinhardi. I. Isotopic transfer experiments with a strain producing eight zoospores. J Mol Biol. 1967 Apr 14;25(1):47–66. doi: 10.1016/0022-2836(67)90278-1. [DOI] [PubMed] [Google Scholar]
  15. VanWinkle-Swift K. P. Uniparental inheritance is promoted by delayed division of the zygote in Chlamydomonas. Nature. 1978 Oct 26;275(5682):749–751. doi: 10.1038/275749a0. [DOI] [PubMed] [Google Scholar]
  16. Williamson D. H., Fennell D. J. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. Methods Cell Biol. 1975;12:335–351. doi: 10.1016/s0091-679x(08)60963-2. [DOI] [PubMed] [Google Scholar]
  17. Williamson D. H., Fennell D. J. Visualization of yeast mitochondrial DNA with the fluorescent stain "DAPI". Methods Enzymol. 1979;56:728–733. doi: 10.1016/0076-6879(79)56065-0. [DOI] [PubMed] [Google Scholar]
  18. Wurtz E. A., Boynton J. E., Gillham N. W. Perturbation of chloroplast DNA amounts and chloroplast gene transmission in Chlamydomonas reinhardtii by 5-fluorodeoxyuridine. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4552–4556. doi: 10.1073/pnas.74.10.4552. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES