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. 1984 Oct;108(2):277–289. doi: 10.1093/genetics/108.2.277

Crosses between Insertion and Point Mutations in λ Gene cI: Stimulation of Neighboring Recombination by Heterology

M Lieb 1,2, M M Tsai 1,2, Richard C Deonier 1,2
PMCID: PMC1202405  PMID: 6094303

Abstract

Intragenic recombination between λcI point mutations and insertions was studied in four-factor crosses. In crosses between two point mutations, there is a linear relationship between recombination frequency and distance. However, in crosses between an insertion and point mutations, there is additional recombination in the regions 200 base pairs to the right and to the left of the insertion. The recombinational stimulation occurred with IS insertions and also with insertions consisting of HindIII fragments of SV40 and with a deletion that removes part of cI. This indicated that the stimulation was a result of heterology per se rather than of information encoded by the insertions. Either Rec or Red functions are sufficient for enhanced recombination near a heterology. The stimulation is attributed to more frequent resolution of recombinational intermediates in the neighborhood of a heterology. "Stalling" of migrating branches or invading strands at a heterology may increase the probability of local DNA cleavage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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