Abstract
Inbred strains of mice carry Ren-1, a gene encoding the thermostable Renin-1 isozyme. Ren-1 is expressed at relatively low levels in mouse submandibular gland and kidney. Some strains also carry Ren-2, a gene encoding the thermolabile Renin-2 isozyme. Ren-2 is expressed at high levels in the mouse submandibular gland and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression. An investigation of renin genes and enzymatic activity in wild-derived mice identified several restriction site polymorphisms as well as putative variants in renin gene expression and protein structure. The number of renin genes carried by different subpopulations of wild-derived mice is consistent with the occurrence of a gene duplication event prior to the divergence of M. spretus (2.75–5.5 million yr ago). This conclusion is in agreement with a prior estimate based upon comparative sequence analysis of Ren-1 and Ren-2 from inbred laboratory mice.
Full Text
The Full Text of this article is available as a PDF (1.4 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bing J., Poulsen K. The renin system in mice. Effects of removal of kidneys or (and) submaxillary glands in different strains. Acta Pathol Microbiol Scand A. 1971;79(2):134–138. [PubMed] [Google Scholar]
- Cohen S., Taylor J. M., Murakami K., Michelakis A. M., Inagami T. Isolation and characterization of renin-like enzymes from mouse submaxillary glands. Biochemistry. 1972 Nov 7;11(23):4286–4293. doi: 10.1021/bi00773a015. [DOI] [PubMed] [Google Scholar]
- Gresik E. W. Postnatal developmental changes in submandibular glands of rats and mice. J Histochem Cytochem. 1980 Aug;28(8):860–870. doi: 10.1177/28.8.6160181. [DOI] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Panthier J. J., Holm I., Rougeon F. The mouse Rn locus: S allele of the renin regulator gene results from a single structural gene duplication. EMBO J. 1982;1(11):1417–1421. doi: 10.1002/j.1460-2075.1982.tb01332.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Piccini N., Knopf J. L., Gross K. W. A DNA polymorphism, consistent with gene duplication, correlates with high renin levels in the mouse submaxillary gland. Cell. 1982 Aug;30(1):205–213. doi: 10.1016/0092-8674(82)90026-5. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Wilson C. M., Cherry M., Taylor B. A., Wilson J. D. Genetic and endocrine control of renin activity in the submaxillary gland of the mouse. Biochem Genet. 1981 Jun;19(5-6):509–523. doi: 10.1007/BF00484623. [DOI] [PubMed] [Google Scholar]
- Wilson C. M., Myhre M. J., Reynolds R. C., Wilson J. D. Regulation of mouse submaxillary gland renin by thyroxine. Endocrinology. 1982 Mar;110(3):982–989. doi: 10.1210/endo-110-3-982. [DOI] [PubMed] [Google Scholar]