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. 1985 Jun;110(2):159–171. doi: 10.1093/genetics/110.2.159

Bacteriophage T4 Gene 32 Participates in Excision Repair as Well as Recombinational Repair of Uv Damages

Gisela Mosig 1
PMCID: PMC1202557  PMID: 3891507

Abstract

Gene 32 of phage T4 has been shown previously to be involved in recombinational repair of UV damages but, based on a mutant study, was thought not to be required for excision repair. However, a comparison of UV-inactivation curves of several gene 32 mutants grown under conditions permissive for progeny production in wild-type or polA - hosts demonstrates that gene 32 participates in both kinds of repair. Different gene 32 mutations differentially inactivate these repair functions. Under conditions permissive for DNA replication and progeny production, all gene 32 mutants investigated here are partially defective in recombinational repair, whereas only two of them, P7 and P401, are also defective in excision repair. P401 is the only mutant whose final slope of the inactivation curve is significantly steeper than that of wildtype T4. These results are discussed in terms of interactions of gp32, a single-stranded DNA-binding protein, with DNA and with other proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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